重组Anti-Ionotropic Glutamate receptor 2抗体[EP966Y] (ab52932)
![Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)](https://www.abcam.cn/ps/products/52/ab52932/Images/ab52932-65438-anti-ionotropic-glutamate-receptor-2-antibody-ep966y-western-blot.jpg "Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP966Y] to Ionotropic Glutamate receptor 2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Ionotropic Glutamate receptor 2抗体[EP966Y]
参阅全部 Ionotropic Glutamate receptor 2 一抗 -
描述
兔单克隆抗体[EP966Y] to Ionotropic Glutamate receptor 2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF,IHC-Fr or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Ionotropic Glutamate receptor 2 aa 1-100 (N terminal). The exact sequence is proprietary.
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阳性对照
- WB: Human brain cell lysate. Human, mouse and rat brain lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP966Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52932于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).
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| 说明 |
|---|
|
WB
1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa). |
应用说明
Is unsuitable for ICC/IF,IHC-Fr or IHC-P.
靶标
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功能
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. -
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily. -
翻译后修饰
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
细胞定位
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. - Information by UniProt
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数据库链接
- Entrez Gene: 2891 Human
- Entrez Gene: 14800 Mouse
- Entrez Gene: 29627 Rat
- Omim: 138247 Human
- SwissProt: P42262 Human
- SwissProt: P23819 Mouse
- SwissProt: P19491 Rat
- Unigene: 32763 Human
see all -
别名
- AMPA 2 antibody
- AMPA selective glutamate receptor 2 antibody
- AMPA-selective glutamate receptor 2 antibody
see all
图片
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Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932) at 1/2000 dilution + Human brain cell lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 99 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)](https://www.abcam.cn/ps/products/52/ab52932/Images/ab52932-65437-anti-ionotropic-glutamate-receptor-2-antibody-ep966y-western-blot.jpg)
Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932) at 1/500 dilution
Lane 1 : Human brain
Lane 2 : Rat brain
Lane 3 : Mouse brain
Predicted band size: 99 kDaSecondary antibody - anti-rabbit HRP (ab6721)
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![Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)](https://www.abcam.cn/ps/products/52/ab52932/Images/ab52932-3-benefits-of-recombinant-antibodies.png)
Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab52932 被引用在 9 文献中.
- Zhang SQ et al. Repeated vagus nerve stimulation produces anxiolytic effects via upregulation of AMPAR function in centrolateral amygdala of male rats. Neurobiol Stress 18:100453 (2022). PubMed: 35685681
- Liu P & Du J Oridonin is an antidepressant molecule working through the PPAR-?/AMPA receptor signaling pathway. Biochem Pharmacol 180:114136 (2020). PubMed: 32628930
- Huang X et al. Methamphetamine abuse impairs motor cortical plasticity and function. Mol Psychiatry 22:1274-1281 (2017). PubMed: 28831198
- Zhang RX et al. EphB2 in the Medial Prefrontal Cortex Regulates Vulnerability to Stress. Neuropsychopharmacology 41:2541-56 (2016). PubMed: 27103064
- Chen C et al. Epigenetic modification of PKM? rescues aging-related cognitive impairment. Sci Rep 6:22096 (2016). WB ; Rat . PubMed: 26926225
- Treccani G et al. Differential expression of postsynaptic NMDA and AMPA receptor subunits in the hippocampus and prefrontal cortex of the flinders sensitive line rat model of depression. Synapse 70:471-4 (2016). PubMed: 27262028
- Luo YX et al. A novel UCS memory retrieval-extinction procedure to inhibit relapse to drug seeking. Nat Commun 6:7675 (2015). WB ; Rat . PubMed: 26169171
- Xue YX et al. Overexpression of Protein Kinase M? in the Prelimbic Cortex Enhances the Formation of Long-Term Fear Memory. Neuropsychopharmacology 40:2146-56 (2015). PubMed: 25722116
- Gascon E et al. Alterations in microRNA-124 and AMPA receptors contribute to social behavioral deficits in frontotemporal dementia. Nat Med 20:1444-51 (2014). WB, IHC ; Mouse . PubMed: 25401692