Anti-Ionotropic Glutamate receptor 2抗体(ab20673)
Key features and details
- Rabbit polyclonal to Ionotropic Glutamate receptor 2
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Ionotropic Glutamate receptor 2抗体
参阅全部 Ionotropic Glutamate receptor 2 一抗 -
描述
兔多克隆抗体to Ionotropic Glutamate receptor 2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat
预测可用于: Chicken, Human
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse GluR2 .
参阅Abcam的专有抗源政策 (Peptide available as ab25708.) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab20673于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 3 - 10 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 3 - 10 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). |
靶标
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功能
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. -
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily. -
翻译后修饰
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
细胞定位
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. - Information by UniProt
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数据库链接
- Entrez Gene: 2891 Human
- Entrez Gene: 14800 Mouse
- Entrez Gene: 29627 Rat
- Omim: 138247 Human
- SwissProt: P42262 Human
- SwissProt: P23819 Mouse
- SwissProt: P19491 Rat
- Unigene: 32763 Human
see all -
别名
- AMPA 2 antibody
- AMPA selective glutamate receptor 2 antibody
- AMPA-selective glutamate receptor 2 antibody
see all
图片
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Western blot - Anti-Ionotropic Glutamate receptor 2 antibody (ab20673)Lane 1 : Marker
Lanes 2-5 : Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml
Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
Lane 3 : Cortex (Mouse) Tissue Lysate at 10 µg
Lane 4 : Brain (Mouse) Tissue Lysate at 10 µg with Mouse Ionotropic Glutamate receptor 2 peptide (ab25708) at 1 µg/ml
Lane 5 : Cortex (Mouse) Tissue Lysate at 10 µg with Mouse Ionotropic Glutamate receptor 2 peptide (ab25708) at 1 µg/ml
Secondary
Lanes 2-5 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 99 kDa
Additional bands: The band observed at ~50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor. We are unsure as to the identity of the band at ~43KDa. -
Western blot - Anti-Ionotropic Glutamate receptor 2 antibody (ab20673)Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 99 kDa
Observed band size: 99 kDa
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.The band observed at 50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor.
数据表及文件
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SDS download
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Datasheet download
文献 (33)
ab20673 被引用在 33 文献中.
- Iemolo A et al. Synaptic mechanisms underlying onset and progression of memory deficits caused by hippocampal and midbrain synucleinopathy. NPJ Parkinsons Dis 9:92 (2023). PubMed: 37328503
- Ford L et al. CPEB3 low-complexity motif regulates local protein synthesis via protein-protein interactions in neuronal ribonucleoprotein granules. Proc Natl Acad Sci U S A 120:e2114747120 (2023). PubMed: 36716374
- Wright AL et al. The Q/R editing site of AMPA receptor GluA2 subunit acts as an epigenetic switch regulating dendritic spines, neurodegeneration and cognitive deficits in Alzheimer's disease. Mol Neurodegener 18:65 (2023). PubMed: 37759260
- Luo L et al. Caveolin-1-Mediated Cholesterol Accumulation Contributes to Exaggerated mGluR-Dependent Long-Term Depression and Impaired Cognition in Fmr1 Knockout Mice. Mol Neurobiol 60:3379-3395 (2023). PubMed: 36854997
- Niikura R et al. Assessments of prolonged effects of desflurane and sevoflurane on motor learning deficits in aged AppNL-G-F/NL-G-F mice. Mol Brain 15:32 (2022). PubMed: 35387663