重组Anti-Ionotropic glutamate receptor 2 + 3抗体[EP929Y] (ab52896)
![Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)](https://www.abcam.cn/ps/products/52/ab52896/Images/ab52896-253596-anti-ionotropic-glutamate-receptor-2-3-antibody-ep929y-western-blot.jpg "Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP929Y] to Ionotropic glutamate receptor 2 + 3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Ionotropic glutamate receptor 2 + 3抗体[EP929Y]
参阅全部 Ionotropic glutamate receptor 2 + 3 一抗 -
描述
兔单克隆抗体[EP929Y] to Ionotropic glutamate receptor 2 + 3 -
宿主
Rabbit -
特异性
ab52896 detects both GluR2 phosphorylated on Serine 880 + GluR3 phosphorylated on Serine 891 of the mature human, and also unphosphorylated GluR2 + 3.
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经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human brain and fetal tissue lysates and mouse and rat brain tissue lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP929Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52896于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/5000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
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| 说明 |
|---|
|
WB
1/2000 - 1/5000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa). |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
靶标
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相关性
Ion channels activated by glutamate are typically divided into two classes. Those sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). AMPAR are comprised of four distinct Glutamate Receptor Subunits (GluR1-4) and they play key roles in almost all excitatory neurotransmission in the brain. The GluR2 subunit is widely expressed throughout the nervous system where it is thought to play key roles in synaptic plasticity and learning and memory. -
细胞定位
Cell Membrane; multi-pass membrane protein. -
数据库链接
- Entrez Gene: 2891 Human
- Entrez Gene: 2892 Human
- Entrez Gene: 14800 Mouse
- Entrez Gene: 29627 Rat
- Omim: 138247 Human
- Omim: 305915 Human
- SwissProt: P42262 Human
- SwissProt: P42263 Human
see all -
别名
- AMPA selective glutamate receptor 2 antibody
- AMPA selective glutamate receptor 3 antibody
- GluR K2 antibody
see all
图片
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Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)All lanes : Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/10000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)](https://www.abcam.cn/ps/products/52/ab52896/Images/ab52896-253595-anti-ionotropic-glutamate-receptor-2-3-antibody-ep929y-western-blot.jpg)
Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/2000 dilution (purified) + Human fetal brain tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)](https://www.abcam.cn/ps/products/52/ab52896/Images/ab52896-65367-anti-ionotropic-glutamate-receptor-2-3-antibody-ep929y-western-blot.gif)
Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896) at 1/5000 dilution (unpurified) + Human brain tissue lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 98 kDa
Observed band size: 98 kDa -
![Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)](https://www.abcam.cn/ps/products/52/ab52896/Images/ab52896-5-benefits-of-recombinant-antibodies.png)
Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (ab52896)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab52896 被引用在 7 文献中.
- Moidunny S et al. Glycogen synthase kinase-3 inhibition rescues sex-dependent contextual fear memory deficit in human immunodeficiency virus-1 transgenic mice. Br J Pharmacol 177:5658-5676 (2020). PubMed: 33080056
- Wei C et al. MicroRNA-330-3p promotes brain metastasis and epithelial-mesenchymal transition via GRIA3 in non-small cell lung cancer. Aging (Albany NY) 11:6734-6761 (2019). PubMed: 31498117
- Yang T et al. Functions of CaBP1 and CaBP2 in the peripheral auditory system. Hear Res 364:48-58 (2018). IHC-P ; Mouse . PubMed: 29661613
- Zhou JY et al. Individual and combined effects of rhynchophylline and ketamine on proliferation, NMDAR1 and GluA2/3 protein expression in PC12 cells. Fitoterapia 85:125-9 (2013). ICC/IF ; Rat . PubMed: 23352746
- Krosnowski K et al. Diverse populations of intrinsic cholinergic interneurons in the mouse olfactory bulb. Neuroscience 213:161-78 (2012). PubMed: 22525133
- Ogino K et al. Duplicated gephyrin genes showing distinct tissue distribution and alternative splicing patterns mediate molybdenum cofactor biosynthesis, glycine receptor clustering, and escape behavior in zebrafish. J Biol Chem 286:806-17 (2011). IHC-Fr ; Zebrafish . PubMed: 20843816
- Graham CE et al. A corticotropin-releasing factor system expressed in the cochlea modulates hearing sensitivity and protects against noise-induced hearing loss. Neurobiol Dis 38:246-58 (2010). IP ; Mouse . PubMed: 20109547