JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB184969

重组Anti-Involucrin抗体[EPR13060(N)] - C-terminal

Anti-Involucrin antibody [EPR13060(N)] - C-terminal

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal Involucrin antibody. C-terminal. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

查看别名

Involucrin, IVL

3 Images
Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)

Intracellular flow cytometric analysis of Involucrin expression in paraformaldehyde-fixed HACAT cells using ab184969 at 1/40 dilution (red) and a rabbit IgG as negative control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)
  • WB

Supplier Data

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)

All lanes:

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969) at 1/10000 dilution

All lanes:

Human fetal skin tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 68 kDa

false

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)
  • WB

Supplier Data

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)

All lanes:

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969) at 1/50000 dilution

All lanes:

HT-1080 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 68 kDa

false

不同偶联物与剂型 (1)

  • Carrier free

    Anti-Involucrin antibody [EPR13060(N)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13060(N)

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

替代产品

Primary Antibodies

AB227530

Anti-Involucrin antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody - N-terminal (AB227530)

  • 0
  • 0
Primary Antibodies

AB234403

Anti-Involucrin antibody [IVRN/2113R]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [IVRN/2113R] (AB234403)

  • 0
  • 0
Primary Antibodies

AB20202

Anti-Involucrin antibody [SY8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY8] (AB20202)

  • 0
  • 0
Primary Antibodies

AB181980

Anti-Involucrin antibody [EPR13054]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13054] (AB181980)

  • 5
  • 1
Primary Antibodies

AB240256

Anti-Involucrin antibody [EPR13054] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13054] - BSA and Azide free (AB240256)

  • 5
  • 1

搭配使用产品

Primary Antibodies

AB137533

Anti-Loricrin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Loricrin antibody (AB137533)

  • 0
  • 0
Primary Antibodies

AB76318

Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (AB76318)

  • 5
  • 14
Primary Antibodies

AB119695

Anti-Cytokeratin 14 antibody [SP53]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 14 antibody [SP53] (AB119695)

  • 5
  • 6
Primary Antibodies

AB221155

Anti-Filaggrin antibody [EPR21892]

BOND RX™ Validated|20ul selling size|Recombinant|Advanced Validation|RabMAb

Multiplex immunohistochemistry - Anti-Filaggrin antibody [EPR21892] (AB221155)

  • 5
  • 3
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/40", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Conditional Ambient
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Involucrin also known as involucrine envocrin or ivrn is a protein with a mass of approximately 68 kDa. You will find involucrin expressed mainly in the suprabasal layers of the stratified squamous epithelia including epidermis tongue and esophagus. This protein becomes cross-linked to form the cornified cell envelope (CCE) in the upper layers of the skin an essential structure for epidermal differentiation and maintenance of skin barrier function.
Biological function summary

Involucrin serves as a critical substrate in the formation of the CCE by providing reactive sites for cross-linking with other proteins like loricrin and small proline-rich proteins. This cross-linking process occurs due to the action of transglutaminases which bond glutamine residues of involucrin with lysine residues in other proteins reinforcing the skin's protective barrier. Involucrin is a part of the protein network integral to keratinocyte terminal differentiation playing a distinctive role in the formation of a resilient and impermeable skin surface.

Pathways

Involucrin contributes directly within the epidermal differentiation complex (EDC) supporting the skin barrier's structural stability and resilience. The protein prominently acts in the terminal differentiation pathway in conjunction with other structural proteins such as filaggrin and keratins. This pathway forms a protective barrier that resists mechanical stress and environmental insults functioning together to provide a robust defense against external factors.

Involucrin's role is highlighted in conditions like psoriasis and ichthyosis where skin barrier formation is disrupted. Aberrant expression or modification of involucrin can lead to compromised barrier integrity leading to these skin disorders. It relates closely to filaggrin in these conditions as both are essential for barrier function and mutations in these proteins can exacerbate skin pathologies. Understanding these connections helps us evaluate potential therapeutic strategies for skin barrier-related diseases.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
See full target information IVL
style
left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cell chemical biology 26:1365-1379.e22 PubMed31422906

2019

Discovery and Characterization of a Cellular Potent Positive Allosteric Modulator of the Polycomb Repressive Complex 1 Chromodomain, CBX7.

Applications

Unspecified application

Species

Unspecified reactive species

Kelsey N Lamb,Daniel Bsteh,Sarah N Dishman,Hagar F Moussa,Huitao Fan,Jacob I Stuckey,Jacqueline L Norris,Stephanie H Cholensky,Dongxu Li,Jingkui Wang,Cari Sagum,Benjamin Z Stanton,Mark T Bedford,Kenneth H Pearce,Terry P Kenakin,Dmitri B Kireev,Gang Greg Wang,Lindsey I James,Oliver Bell,Stephen V Frye

Journal of cellular biochemistry 120:9213-9229 PubMed30556162

2018

Peroxisome proliferator-activated receptor-α agonist and all-trans retinoic acid induce epithelial differentiation of subcutaneous adipose-derived stem cells from debrided burn skin.

Applications

Unspecified application

Species

Unspecified reactive species

Shanmugasundaram Natesan,Nicole L Wrice,Robert J Christy
View all publications
style
left-column
style
left-column
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com