Anti-Interferon gamma抗体(ab9657)
Key features and details
- Rabbit polyclonal to Interferon gamma
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Interferon gamma抗体
参阅全部 Interferon gamma 一抗 -
描述
兔多克隆抗体to Interferon gamma -
宿主
Rabbit -
经测试应用
适用于: Sandwich ELISA, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human Interferon gamma. Mature Interferon gamma is from aa 24-161 of SwissProt ID Q14609. aa 1-23 represents the signal peptide.
Database link: Q14609 -
阳性对照
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常规说明
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab231036.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200µl of sterile water. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
No preservative, sterile filtered -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
affinity chromatography employing immobilized Human IFN-gamma matrix. -
克隆
多克隆 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
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Isotype control
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Positive Controls
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
Detects IFN-gamma by sandwich ELISA using 100µl/well antibody solution. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIFN-gamma. Can be paired for ELISA with ab9658. |
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| WB | (4) |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 17 kDa.
Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| 说明 |
|---|
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Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. Detects IFN-gamma by sandwich ELISA using 100µl/well antibody solution. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIFN-gamma. Can be paired for ELISA with ab9658. |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 17 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
靶标
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功能
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
组织特异性
Released primarily from activated T lymphocytes. -
疾病相关
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
序列相似性
Belongs to the type II (or gamma) interferon family. -
翻译后修饰
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
- Unigene: 856 Human
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别名
- IF 1 antibody
- IFG antibody
- IFI antibody
see all
图片
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Western blot - Anti-Interferon gamma antibody (ab9657)Lanes 2-12 : Anti-Interferon gamma antibody (ab9657) at 0.1 µg/ml
Lane 1 : Marker
Lanes 2-12 :Recombinant human Interferon gamma protein (Active) (ab9659)
Performed under non-reducing conditions.
Predicted band size: 17 kDa -
Sandwich ELISA - Anti-Interferon gamma antibody (ab9657)To detect Human IFN-γ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with a compatible secondary detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-γ.
实验方案
数据表及文件
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Datasheet download
文献 (105)
ab9657 被引用在 105 文献中.
- Sandoval Pacheco CM et al. Role of antigen-presenting cells in non-ulcerated skin lesions caused by Leishmania (Leishmania) infantum chagasi. Parasite Immunol 45:e12971 (2023). PubMed: 36695719
- Jin Q et al. MYC promotes fibroblast osteogenesis by regulating ALP and BMP2 to participate in ectopic ossification of ankylosing spondylitis. Arthritis Res Ther 25:28 (2023). PubMed: 36803548
- An HJ et al. STAT3/NF‑κB decoy oligodeoxynucleotides inhibit atherosclerosis through regulation of the STAT/NF‑κB signaling pathway in a mouse model of atherosclerosis. Int J Mol Med 51:N/A (2023). PubMed: 37026512
- Hashimoto K et al. Clinicopathological assessment of PD-1/PD-L1 immune checkpoint expression in desmoid tumors. Eur J Histochem 67:N/A (2023). PubMed: 37098880
- Takizawa H et al. Role of TGF-beta1 and TNF-alpha1 produced by neoplastic cells in the pathogenesis of fibrosis in patients with hematologic neoplasms. J Clin Exp Hematop 63:83-89 (2023). PubMed: 37121717
