重组Anti-Interferon alpha/beta receptor 1抗体[EPR6244] (ab124764)
![Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)](https://www.abcam.cn/ps/products/124/ab124764/Images/ab124764-521037-anti-interferon-alphabeta-receptor-1-antibody-epr6244-western-blot-wild-type-hela-human.jpg "Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6244] to Interferon alpha/beta receptor 1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Interferon alpha/beta receptor 1抗体[EPR6244]
参阅全部 Interferon alpha/beta receptor 1 一抗 -
描述
兔单克隆抗体[EPR6244] to Interferon alpha/beta receptor 1 -
宿主
Rabbit -
特异性
In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples. -
经测试应用
适用于: WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Interferon alpha/beta receptor 1 aa 100-200. The exact sequence is proprietary.
Database link: P17181 -
阳性对照
- HeLa, HeLa treated with IFN-alpha, K562 and U937 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 2.40 x 10 -12 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6244 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124764于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 90-130 kDa (predicted molecular weight: 64 kDa).
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 90-130 kDa (predicted molecular weight: 64 kDa). |
靶标
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功能
Associates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves. -
组织特异性
IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266S. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R. -
序列相似性
Belongs to the type II cytokine receptor family.
Contains 3 fibronectin type-III domains. -
翻译后修饰
Phosphorylated on tyrosine residues by TYK2 tyrosine kinase. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3454 Human
- Omim: 107450 Human
- SwissProt: P17181 Human
- Unigene: 529400 Human
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别名
- Alpha type antiviral protein antibody
- Antiviral protein, alpha-type antibody
- Antiviral protein, beta-type antibody
see all
图片
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Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : IFNAR1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 116 kDa why is the actual band size different from the predicted?Western blot: Anti-IFNAR1 antibody [EPR6244] (ab124764) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab124764 was shown to bind specifically to IFNAR1. A band was observed at 116 kDa in wild-type HeLa cell lysates with no signal observed at this size in IFNAR1 knockout cell line. To generate this image, wild-type and IFNAR1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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![Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)](https://www.abcam.cn/ps/products/124/ab124764/Images/ab124764-478990-AntiInterferon-alpha-beta-receptor-1-antibody-Western-Blot.png)
Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution
Lane 1 : Untreated K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate. with NFDM/TBST
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) treated with PNGase F whole cell lysate. with NFDM/TBST
Lane 3 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate. with NFDM/TBST
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with PNGase F whole cell lysate 15 µg. with NFDM/TBST
Lysates/proteins at 15 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated.)
Predicted band size: 64 kDa
Observed band size: 64.13 kDa why is the actual band size different from the predicted?
Exposure time: 180 seconds -
![Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)](https://www.abcam.cn/ps/products/124/ab124764/Images/ab124764-257152-anti-interferon-alpha-beta-receptor-1-antibody-epr6244-western-blot.jpg)
Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit monoclonal [EPR6244] to Interferon alpha/beta receptor 1 (ab124764) at 1/1000 dilution
Predicted band size: 64 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBSTIn mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.
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![Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)](https://www.abcam.cn/ps/products/124/ab124764/Images/ab124764-257151-anti-interferon-alpha-beta-receptor-1-antibody-epr6244-western-blot.jpg)
Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (purified)
Lane 1 : HEK-293 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : LNCaP whole cell lysate
Lane 4 : SH-SY5Y whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 90-130 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)](https://www.abcam.cn/ps/products/124/ab124764/Images/ab124764-256793-anti-interferon-alpha-beta-receptor-1-antibody-epr6244-western-blot.jpg)
Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (unpurified)
Lane 1 : Mouse brain whole tissue lysate
Lane 2 : Mouse heart whole tissue lysate
Lane 3 : Mouse kidney whole tissue lysate
Lane 4 : Mouse spleen whole tissue lysate
Lane 5 : Rat brain whole tissue lysate
Lane 6 : Rat heart whole cell lysate
Lane 7 : Rat kidney whole cell lysate
Lane 8 : Rat spleen whole tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (H+L), peroxidase conjugated at 1/2000 dilution
Predicted band size: 64 kDa
Exposure time: 3 minutesBlocking buffer: 5% NFDM/TBST.
In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.
-
![Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)](https://www.abcam.cn/ps/products/124/ab124764/Images/ab124764-138612-anti-interferon-alpha-beta-receptor-1-antibody-epr6244-western-blot.jpg)
Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (Unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with IFN-alpha
Lane 3 : K562 cell lysate
Lane 4 : U937 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/200 dilution
Predicted band size: 64 kDa -
![OI-RD Scanning - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)](https://www.abcam.cn/ps/products/124/ab124764/Images/ab124764-138611-anti-interferon-alpha-beta-receptor-1-antibody-epr6244.jpg)
OI-RD Scanning - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) -
![Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)](https://www.abcam.cn/ps/products/124/ab124764/Images/ab124764-7-benefits-of-recombinant-antibodies.png)
Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab124764 被引用在 13 文献中.
- Vila IK et al. STING orchestrates the crosstalk between polyunsaturated fatty acid metabolism and inflammatory responses. Cell Metab 34:125-139.e8 (2022). PubMed: 34986331
- Hu B et al. IFNα Potentiates Anti-PD-1 Efficacy by Remodeling Glucose Metabolism in the Hepatocellular Carcinoma Microenvironment. Cancer Discov 12:1718-1741 (2022). PubMed: 35412588
- Wu W et al. MicroRNA let-7 Suppresses Influenza A Virus Infection by Targeting RPS16 and Enhancing Type I Interferon Response. Front Cell Infect Microbiol 12:904775 (2022). PubMed: 35873150
- Tu X et al. Interruption of post-Golgi STING trafficking activates tonic interferon signaling. Nat Commun 13:6977 (2022). PubMed: 36379959
- Fueyo-González F et al. Interferon-β acts directly on T cells to prolong allograft survival by enhancing regulatory T cell induction through Foxp3 acetylation. Immunity 55:459-474.e7 (2022). PubMed: 35148827