Anti-Interferon alpha 2b抗体[4E10] (ab9388)

Thank you for contacting us. Yes, these are the same lot numbers in all cases. The number after the - just indicates the time it was aliquotted, so if the GRXXXX is the same but the dash is different you can be assured the lot is still the same. Please let me know if you have any further questions.

Thank you for contacting us.

We have used this antibody under reducing and denaturing conditions in western blot. We unfortunately haven't done any comparison studies between native vs reducing conditions. I am sorry we are unable to comment on this due to lack of tested data.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us. Concentrations of the antibodies ab9386 and ab9388 are determined spectrophotometrically by an adsorbance measurement at 280 nm. The absorbance value (A280) is divided by the extiction coefficient of mouse IgG - 1.35. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting Abcam. For both ab9386 and ab9388, we currently sell them in 100ul aliquots, at a concentration of 2mg/ml. From what I can see, there are no plans to change this concentration for future lots of the antibody and since it is a mouse monoclonal there should not be a large variation in batch to batch concentrations. If any changes do occur then the new concentration will be clearly displayed on the datasheet and/or vial. Please let me know if there is anything else I can help you with.

Thank you for your enquiry. We currently do not have an antibody to human interferon alpha that is biotinylated. We have the following human interferon alpha antibodies: ab9660, ab9386, ab8317, ab9388, and ab5258 (HRP conjugated). If you have any more questions, please contact us again.

To use monoclonal antibodies 9D3 and 4E10 in sandwich ELISA for the detection of huIFN-alpha-2b, 4E10 antibody should be labeled either with horse-radish peroxidase or biotin. Monoclonal antibody 9D3 should be used as a capture antibody (for plate coating). Before labeling procedure, antibody 4E10 should be dialysed to remove sodium azide (see Abcam protocol Azide Removal by Dialysis). Biotinylation protocols are available on-line (see, for example: www.protocol-online.org or www.drmr.com). Biotinylated 4E10 can be detected using Streptavidin-Peroxidase (widely used reagent, see, for example www.chemicom.com or www.sigmaaldrich.com). Labeling of monoclonal antibody with peroxidase is more complicated procedure than biotinylation (see, for example, HRP Conjugation Kit description at www.4adi.com). After preparation of 4E10 conjugate, it can be used together with 9D3 in sandwich ELISA for the detection of huIFN-alpha-2b. Short protocol is given below: 1. Plate coating: 9D3, 5 mcg/ml in Na-carbonate buffer (pH 9.5), 100 mcl/well, incubation overnight at +4 C; 2. Plate blocking: 2% BSA in PBS, 100 mcl/well, 1 h at RT; 3. Washing: 3 times with PBST (PBS with 0.1% Tween-20); 4. Incubation with IFN standard and test samples: 1 h at RT, 100 mcl/well, dilution buffer – PBST; 5. Washing: 5-6 times with PBST; 6. Incubation with 4E10 conjugate: 1 h at RT, dilution buffer – PBST. If 4E10-biotin is used, further incubation step with Streptavidin-Peroxidase is required; 7. Washing: 8-10 times with PBST; 8. Development of enzymatic reaction using TMB-substrate.