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使用敲除细胞株进行验证重组RabMAb

重组Anti-Integrin linked ILK抗体[EP1593Y] - BSA and Azide free (ab271842)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] - BSA and Azide free (ab271842)
  • Immunoprecipitation - Anti-Integrin linked ILK antibody [EP1593Y] - BSA and Azide free (ab271842)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] - BSA and Azide free (ab271842)
  • Anti-Integrin linked ILK antibody [EP1593Y] - BSA and Azide free (ab271842)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1593Y] to Integrin linked ILK - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Rat, Human, African green monkey

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Integrin linked ILK抗体[EP1593Y] - BSA and Azide free
    参阅全部 Integrin linked ILK 一抗

  • 描述

    兔单克隆抗体[EP1593Y] to Integrin linked ILK - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC/IF

  • 种属反应性

    与反应: Rat, Human, African green monkey
    预测可用于: Mouse, Cow

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Human heart muscle tissue and COS cell lysate

  • 常规说明

    ab271842 is the carrier-free version of ab52480.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • 存储溶液

    pH: 7.20
    Constituent: PBS

  • 无载体

    是
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EP1593Y

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab271842于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 51 kDa.

For unpurified use at 1/2000 - 1/10000 dilution.&nbsp;

 
IP
Use at an assay dependent concentration.

For unpurified use at 1/30 dilution.&nbsp;

 
IHC-P
Use at an assay dependent concentration.

See IHC antigen retrieval protocols.The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

 
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 51 kDa.

For unpurified use at 1/2000 - 1/10000 dilution.&nbsp;

 
IP
Use at an assay dependent concentration.

For unpurified use at 1/30 dilution.&nbsp;

 
IHC-P
Use at an assay dependent concentration.

See IHC antigen retrieval protocols.The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

 
应用说明
Is unsuitable for Flow Cyt or ICC/IF.

靶标

  • 功能

    Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.

  • 组织特异性

    Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.

  • 序列相似性

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 5 ANK repeats.
    Contains 1 protein kinase domain.

  • 结构域

    A PH-like domain is involved in phosphatidylinositol phosphate binding.

  • 翻译后修饰

    Autophosphorylated on serine residues.

  • 细胞定位

    Cell junction > focal adhesion. Cell membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • 59 kDa serine/threonine protein kinase antibody
    • 59 kDa serine/threonine-protein kinase antibody
    • DKFZp686F1765 antibody
    • Epididymis secretory protein Li 28 antibody
    • HEL S 28 antibody
    • ILK 1 antibody
    • ILK 2 antibody
    • ILK antibody
    • ILK-1 antibody
    • ILK-2 antibody
    • ILK_HUMAN antibody
    • ILK1 antibody
    • ILK2 antibody
    • Integrin linked kinase 2 antibody
    • Integrin linked Kinase antibody
    • Integrin linked protein kinase antibody
    • Integrin-linked protein kinase antibody
    • p59 antibody
    • p59ILK antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] - BSA and Azide free (ab271842)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] - BSA and Azide free (ab271842)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer tissue sections labeling Integrin linked ILK with Purified ab52480 at 1/100 dilution (7.13 μg/ml). Heat mediated antigen retrieval was performed using citrate (pH 6.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52480).
  • Immunoprecipitation - Anti-Integrin linked ILK antibody [EP1593Y] - BSA and Azide free (ab271842)
    Immunoprecipitation - Anti-Integrin linked ILK antibody [EP1593Y] - BSA and Azide free (ab271842)

    ab52480 (purified) at 1:40 dilution (2µg) immunoprecipitating Integrin linked ILK in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab52480 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52480 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52480).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] - BSA and Azide free (ab271842)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] - BSA and Azide free (ab271842)

    Immunohistochemical analysis of paraffin-embedded human heart muscle using unpurified ab52480 at a 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52480).
  • Anti-Integrin linked ILK antibody [EP1593Y] - BSA and Azide free (ab271842)
    Anti-Integrin linked ILK antibody [EP1593Y] - BSA and Azide free (ab271842)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

发表研究结果有使用 ab271842？请让我们知道，以便我们可以引用本数据表中的参考文章。

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