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使用敲除细胞株进行验证重组RabMAb

重组Anti-Integrin linked ILK抗体[EP1593Y] (ab52480)

Submit a review Q&A (1)References (18)
Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
  • Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
  • Immunoprecipitation - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
  • Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
  • Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
  • Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
  • Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
  • Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1593Y] to Integrin linked ILK
  • Suitable for: WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Rat, Human, African green monkey

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Integrin linked ILK抗体[EP1593Y]
    参阅全部 Integrin linked ILK 一抗

  • 描述

    兔单克隆抗体[EP1593Y] to Integrin linked ILK

  • 宿主

    Rabbit

  • 特异性

    This antibody reacts with Integrin linked ILK. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • 经测试应用

    适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC/IF

  • 种属反应性

    与反应: Rat, Human, African green monkey
    预测可用于: Mouse, Cow

  • 免疫原

    Synthetic peptide within Human Integrin linked ILK aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13418

  • 阳性对照

    • Human heart muscle tissue and COS cell lysate

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EP1593Y

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab52480于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

For unpurified use at 1/2000 - 1/10000 dilution. 
IP
1/40.

For unpurified use at 1/30 dilution. 
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
说明
WB
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

For unpurified use at 1/2000 - 1/10000 dilution. 
IP
1/40.

For unpurified use at 1/30 dilution. 
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
应用说明
Is unsuitable for Flow Cyt or ICC/IF.

靶标

  • 功能

    Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.

  • 组织特异性

    Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.

  • 序列相似性

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 5 ANK repeats.
    Contains 1 protein kinase domain.

  • 结构域

    A PH-like domain is involved in phosphatidylinositol phosphate binding.

  • 翻译后修饰

    Autophosphorylated on serine residues.

  • 细胞定位

    Cell junction > focal adhesion. Cell membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • 59 kDa serine/threonine protein kinase antibody
    • 59 kDa serine/threonine-protein kinase antibody
    • DKFZp686F1765 antibody
    • Epididymis secretory protein Li 28 antibody
    • HEL S 28 antibody
    • ILK 1 antibody
    • ILK 2 antibody
    • ILK antibody
    • ILK-1 antibody
    • ILK-2 antibody
    • ILK_HUMAN antibody
    • ILK1 antibody
    • ILK2 antibody
    • Integrin linked kinase 2 antibody
    • Integrin linked Kinase antibody
    • Integrin linked protein kinase antibody
    • Integrin-linked protein kinase antibody
    • p59 antibody
    • p59ILK antibody
    see all

图片

  • Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
    Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Integrin linked ILK knockout HAP1 cell lysate (20 µg)
    Lane 3: HEK293 cell lysate (20 µg)
    Lane 4: K562 cell lysate (20 µg) 
    Lanes 1 - 4: Merged signal (red and green). Green - ab52480 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.

    Unpurified ab52480 was shown to specifically react with Integrin linked ILK when Integrin linked ILK knockout samples were used. Wild-type and Integrin linked ILK knockout samples were subjected to SDS-PAGE. Unpurified ab52480 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer tissue sections labeling Integrin linked ILK with Purified ab52480 at 1/100 dilution (7.13 μg/ml). Heat mediated antigen retrieval was performed using citrate (pH 6.0).

  • Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
    Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
    All lanes : Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates with 5% NFDM/TBST
    Lane 3 : Mouse heart lysates with 5% NFDM/TBST
    Lane 4 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates with 5% NFDM/TBST
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

  • Immunoprecipitation - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
    Immunoprecipitation - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

    ab52480 (purified) at 1:40 dilution (2µg) immunoprecipitating Integrin linked ILK in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab52480 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52480 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
    Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
    Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/1000 dilution (Purified) + COS-1 (African green monkey kidney fibroblast-like) whole cell lysates at 15 µg with 5% NFDM/TBST

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

  • Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
    Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
    Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/2000 dilution (unpurified) + COS-1 (African green monkey kidney fibroblast-like) cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

  • Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
    Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
    Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) (unpurified) + PC12

    Predicted band size: 51 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

    Immunohistochemical analysis of paraffin-embedded human heart muscle using unpurified ab52480 at a 1/50 dilution.

  • Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
    Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
    All lanes : Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/2000 dilution (unpurified)

    Lane 1 : Rat Brain
    Lane 2 : Rat Heart
    Lane 3 : Rat Kidney
    Lane 4 : Rat Spleen

    Predicted band size: 51 kDa


    Exposure time: 3 minutes
  • Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)
    Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (18)

发表研究结果有使用 ab52480？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab52480 被引用在 18 文献中.

  • Beetham H  et al. Loss of Integrin-Linked Kinase Sensitizes Breast Cancer to SRC Inhibitors. Cancer Res 82:632-647 (2022). PubMed: 34921014
  • Yu Y  et al. Synergistic effect of Tripterygium glycosides and cisplatin on drug-resistant human epithelial ovarian cancer via ILK/GSK3β/Slug signal pathway. Am J Transl Res 14:2051-2062 (2022). PubMed: 35422913
  • Chen H  et al. GDC-0941 activates integrin linked kinase (ILK) expression to cause resistance to GDC-0941 in breast cancer by the tumor necrosis factor (TNF)-α signaling pathway. Bioengineered 13:10944-10955 (2022). PubMed: 35477364
  • Yue Y  et al. Interleukin-10 Deficiency Alters Endothelial Progenitor Cell-Derived Exosome Reparative Effect on Myocardial Repair via Integrin-Linked Kinase Enrichment. Circ Res 126:315-329 (2020). PubMed: 31815595
  • Chen Q  et al. Integrin-linked kinase improves uterine receptivity formation by activating Wnt/ß-catenin signaling and up-regulating MMP-3/9 expression. Am J Transl Res 12:3011-3022 (2020). PubMed: 32655826
View all Publications for this product

客户评价及客户问答

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Question

A customer has recently purchased this antibody from us and would like to know the concentration.



The lot number is GR44532

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Answer

Thank you for contacting us.

Unfortunately, we don't know the concentration ourselves: This is a tissue culture supernatant, and it is impossible to measure the concentration due to all the proteins in this solution.

Please guide them to our Technical frequently asked questions (FAQs) site: We have a table in which we estimate the concentration ofnon-purified antibodies. In this case, the concentration range is approx. 1 - 3 mg/ml

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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