Anti-Integrin linked ILK抗体(ab74336)
Key features and details
- Rabbit polyclonal to Integrin linked ILK
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Integrin linked ILK抗体
参阅全部 Integrin linked ILK 一抗 -
描述
兔多克隆抗体to Integrin linked ILK -
宿主
Rabbit -
经测试应用
适用于: WB, IP, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Medaka fish
-
免疫原
Synthetic peptide corresponding to a region between residues 402 and 451 of human Integrin linked ILK (NP_004508.1)
-
阳性对照
- HeLa, 293T and NIH3T3 whole cell lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab74336 was affinity purified using an epitope specific to Integrin linked ILK immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74336于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 51 kDa.
|
|
| IP |
Use at 2-5 µg/mg of lysate.
|
|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 51 kDa. |
|
IP
Use at 2-5 µg/mg of lysate. |
|
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
-
功能
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. -
组织特异性
Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain. -
结构域
A PH-like domain is involved in phosphatidylinositol phosphate binding. -
翻译后修饰
Autophosphorylated on serine residues. -
细胞定位
Cell junction > focal adhesion. Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 540207 Cow
- Entrez Gene: 3611 Human
- Entrez Gene: 16202 Mouse
- Entrez Gene: 170922 Rat
- Omim: 602366 Human
- SwissProt: Q3SWY2 Cow
- SwissProt: Q13418 Human
- SwissProt: O55222 Mouse
see all -
别名
- 59 kDa serine/threonine protein kinase antibody
- 59 kDa serine/threonine-protein kinase antibody
- DKFZp686F1765 antibody
see all
图片
-
Western blot - Anti-Integrin linked ILK antibody (ab74336)All lanes : Anti-Integrin linked ILK antibody (ab74336) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 10 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody (ab74336)Immunocytochemistry/Immunofluorescence analysis of acetone-fixed asynchronous HeLa cells labelling Integrin linked ILK with ab74336 at 1/100 (2µg/ml). A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody. -
Immunoprecipitation - Anti-Integrin linked ILK antibody (ab74336)Detection of Integrin linked ILK by Western Blot of Immunprecipitate.
ab74336 at 1µg/ml staining Integrin linked ILK in HeLa whole cell lysate immunoprecipitated using ab74336 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds. -
Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody (ab74336)ICC/IF image of ab74336 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74336, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab74336 被引用在 3 文献中.
- Huang Y & Gunst SJ Phenotype transitions induced by mechanical stimuli in airway smooth muscle are regulated by differential interactions of parvin isoforms with paxillin and Akt. Am J Physiol Lung Cell Mol Physiol 318:L1036-L1055 (2020). PubMed: 32130030
- Manak MS et al. Live-cell phenotypic-biomarker microfluidic assay for the risk stratification of cancer patients via machine learning. Nat Biomed Eng 2:761-772 (2018). PubMed: 30854249
- Lin F et al. Echistatin prevents posterior capsule opacification in diabetic rabbit model via integrin linked kinase signaling pathway. Int J Clin Exp Pathol 8:14294-304 (2015). PubMed: 26823745