重组Anti-Integrin beta 4抗体[EPR8559] (ab133682)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8559] to Integrin beta 4
- Suitable for: ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Integrin beta 4抗体[EPR8559]
参阅全部 Integrin beta 4 一抗 -
描述
兔单克隆抗体[EPR8559] to Integrin beta 4 -
宿主
Rabbit -
经测试应用
适用于: ICC/IFmore details
不适用于: Flow Cyt,IHC-P,IP or WB -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Integrin beta 4 aa 1750-1850 (C terminal). The exact sequence is proprietary.
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阳性对照
- BxPC-3 and SW480 cell lysates; BxPC-3 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 9.70 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8559 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Integrin beta 4 antibody [EPR8559] - BSA and Azide free (ab240078)
- Alexa Fluor® 488 Anti-Integrin beta 4 antibody [EPR8559] (ab320434)
- Alexa Fluor® 647 Anti-Integrin beta 4 antibody [EPR8559] (ab320435)
- Alexa Fluor® 555 Anti-Integrin beta 4 antibody [EPR8559] (ab320436)
- Alexa Fluor® 594 Anti-Integrin beta 4 antibody [EPR8559] (ab320437)
- PE Anti-Integrin beta 4 antibody [EPR8559] (ab320438)
- APC Anti-Integrin beta 4 antibody [EPR8559] (ab320439)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133682于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (1) |
1/250 - 1/500.
|
说明 |
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ICC/IF
1/250 - 1/500. |
靶标
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功能
Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. -
组织特异性
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach. -
疾病相关
Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities. -
序列相似性
Belongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain. -
结构域
The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3691 Human
- Omim: 147557 Human
- SwissProt: P16144 Human
- Unigene: 632226 Human
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别名
- CD 104 antibody
- CD104 antibody
- CD104 antigen antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)This image is courtesy of an Abreview submitted by Qingsu Cheng
ab133682 staining Integrin beta 4 in human breast epithelail cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
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Immunofluorescent analysis of BxPC-3 cells labelling Integrin beta 4 with ab133682 at 1/250 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab133682 被引用在 12 文献中.
- Wang H et al. 7-Ketocholesterol Promotes Retinal Pigment Epithelium Senescence and Fibrosis of Choroidal Neovascularization via IQGAP1 Phosphorylation-Dependent Signaling. Int J Mol Sci 24:N/A (2023). PubMed: 37373423
- Hao N et al. Laminin-integrin a6b4 interaction activates notch signaling to facilitate bladder cancer development. BMC Cancer 22:558 (2022). PubMed: 35585515
- Xu K et al. ITGB4 deficiency induces DNA damage by downregulating HDAC1 in airway epithelial cells under stress stimulation. Pediatr Allergy Immunol 33:e13871 (2022). PubMed: 36282138
- Lakshmanan I et al. ST6GalNAc-I promotes lung cancer metastasis by altering MUC5AC sialylation. Mol Oncol 15:1866-1881 (2021). PubMed: 33792183
- Gao Q et al. Functional Passenger-Strand miRNAs in Exosomes Derived from Human Colon Cancer Cells and Their Heterogeneous Paracrine Effects. Int J Biol Sci 16:1044-1058 (2020). PubMed: 32140072