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Anti-Integrin beta 4抗体[439-9B] (ab110167)

Reviews (2)Q&A (1)References (12)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [439-9B] (ab110167)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [439-9B] (ab110167)
  • Flow Cytometry - Anti-Integrin beta 4 antibody [439-9B] (ab110167)

Key features and details

  • Rat monoclonal [439-9B] to Integrin beta 4
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2b

Conjugates logo Related conjugates and formulations

Biotin

概述

  • 产品名称

    Anti-Integrin beta 4抗体[439-9B]
    参阅全部 Integrin beta 4 一抗

  • 描述

    大鼠单克隆抗体[439-9B] to Integrin beta 4

  • 宿主

    Rat

  • 特异性

    Recognizes human Integrin beta-4, a 202 kDa subunit that associates with integrin alpha 6 (CD49f), thereby forming the laminin receptor. Multiple splice variants have been identified. It is used as a surface staining antibody.

  • 经测试应用

    适用于: IHC-P, Flow Cytmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 阳性对照

    • Human Pancreas and Human Placenta tissues, A549 cell line

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 克隆

    单克隆

  • 克隆编号

    439-9B

  • 同种型

    IgG2b

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab110167于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use 1µg for 105-8 cells.

in 100 µl

 

 

 

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 
说明
IHC-P
Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use 1µg for 105-8 cells.

in 100 µl

 

 

 

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

靶标

  • 功能

    Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.

  • 组织特异性

    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.

  • 疾病相关

    Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.

  • 序列相似性

    Belongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.

  • 结构域

    The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CD 104 antibody
      • CD104 antibody
      • CD104 antigen antibody
      • gp150 antibody
      • Integrin beta 4 subunit antibody
      • Integrin beta-4 antibody
      • ITB4_HUMAN antibody
      • ITG B4 antibody
      • ITGB 4 antibody
      • Itgb4 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [439-9B] (ab110167)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [439-9B] (ab110167)

      ab110167 at 20 µg/ml staining integrin beta 4 in Formalin-Fixed, Paraffin-Embedded Human Pancreas(ab29816). Sections were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [439-9B] (ab110167)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [439-9B] (ab110167)

      ab110167 at 20 µg/ml staining integrin beta 4 in Formalin-Fixed, Paraffin-Embedded Human Placenta. Sections were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

    • Flow Cytometry - Anti-Integrin beta 4 antibody [439-9B] (ab110167)
      Flow Cytometry - Anti-Integrin beta 4 antibody [439-9B] (ab110167)

      Staining of the A549 cell line with 0.5 ug of Rat IgG2b Isotype Control (ab98984) (blue histogram) or 0.5 ug of ab110167 (purple histogram) followed by Biotin Anti-Rat IgG and Sav-PE. Total viable cells were used for analysis.

    文献 (12)

    发表研究结果有使用 ab110167？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab110167 被引用在 12 文献中.

    • Benedetti G  et al. Generation of human gastric assembloids from primary fetal organoids. Pediatr Surg Int 40:6 (2023). PubMed: 37999863
    • Walker MR  et al. O-linked α2,3 sialylation defines stem cell populations in breast cancer. Sci Adv 8:eabj9513 (2022). PubMed: 34995107
    • Choi SH  et al. KRAS Mutants Upregulate Integrin β4 to Promote Invasion and Metastasis in Colorectal Cancer. Mol Cancer Res 20:1305-1319 (2022). PubMed: 35394541
    • Riivari S  et al. Epithelial cell attachment and adhesion protein expression on novel in sol TiO2 coated zirconia and titanium alloy surfaces. J Biomed Mater Res B Appl Biomater 110:2533-2541 (2022). PubMed: 35730701
    • Kumar U  et al. MicroRNA-495/TGF-β/FOXC1 axis regulates multidrug resistance in metaplastic breast cancer cells. Biochem Pharmacol 192:114692 (2021). PubMed: 34298004
    View all Publications for this product

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin beta 4 antibody [439-9B]

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (184A1 mammary epithelial)
    Permeabilization
    Yes - PBS + 0.25% Triton-X
    Specification
    184A1 mammary epithelial
    Blocking step
    (agent) for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 19 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin beta 4 antibody [439-9B]

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Hepatocellular carcinoma)
    Permeabilization
    Yes - Triton X-100
    Specification
    Hepatocellular carcinoma
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 22 2018

    Question

    Please send two testing discount codes for two integrin beta 4 antibodies for flow cytometry of mouse samples.

    Read More
    Answer

    DISCOUNT CODES:

    ab25254 - ***
    ab110167 - ***

    Expiration date: ***

    I am very pleased to hear you would like to accept our offer and test ab25254 in flow cytometry, and ab110167 on mouse samples. To redeem this offer, please submit Abreviews for flow cytometry on mouse samples and include the appropriate code in the “Additional Comments” section so we know the Abreviews are for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    We publish positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com