重组Anti-Integrin beta 3抗体[Y2/51] (ab9509)
![Flow Cytometry - Anti-Integrin beta 3 antibody [Y2/51] (ab9509)](https://www.abcam.cn/ps/products/9/ab9509/Images/ab9509-535434-anti-integrin-beta-3-antibody-y2-51flow-cytometry-pbmc-human.jpg "Flow Cytometry - Anti-Integrin beta 3 antibody [Y2/51] (ab9509)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [Y2/51] to Integrin beta 3
- Suitable for: Flow Cyt
- Reacts with: Human
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Integrin beta 3抗体[Y2/51]
参阅全部 Integrin beta 3 一抗 -
描述
小鼠单克隆抗体[Y2/51] to Integrin beta 3 -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details
不适用于: ICC/IF,IHC-P or WB -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human Integrin beta 3. PHA-stimulated human mononuclear cells.
-
阳性对照
- Flow cyto: Human peripheral blood mononuclear cell (PBMC) cells
-
常规说明
This product has been switch from hybridoma to recombinant on 25th March 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y2/51 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
1/1000.
|
| 说明 |
|---|
|
Flow Cyt
1/1000. |
靶标
-
功能
Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
组织特异性
Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis. -
疾病相关
Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors. -
序列相似性
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
翻译后修饰
Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 3690 Human
- Omim: 173470 Human
- SwissProt: P05106 Human
- Unigene: 218040 Human
-
别名
- BDPLT16 antibody
- BDPLT2 antibody
- CD 61 antibody
see all
图片
-
Flow Cytometry - Anti-Integrin beta 3 antibody [Y2/51] (ab9509)
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling Integrin beta 3 with ab9509 at 1/1000 dilution (0.1 ug) / Right compared with a Mouse monoclonal IgG / Left.
Goat anti mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary antibody at 1/2000 dilution.
Cells were stained with mouse IgG or ab9509. Then stained with anti-CD41 conjugated to APC.
Gated on viable cells. -
![Flow Cytometry - Anti-Integrin beta 3 antibody [Y2/51] (ab9509)](https://www.abcam.cn/ps/products/9/ab9509/Images/ab9509-535435-anti-integrin-beta-3-antibody-y2-51flow-cytometry-hela-cells-human.jpg)
Flow Cytometry - Anti-Integrin beta 3 antibody [Y2/51] (ab9509)Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell, Left) / HUVEC (Human umbilical vein endothelial cell, Right) cells labelling Integrin beta 3 with ab9509 at 1/1000 dilution (0.1 ug)/Red compared with a Mouse monoclonal IgG / Black isotype control and an unlabelled control (Cell without incubation with primary antibody and secondary antibody / Blue).
Secondary antibody was Goat anti mouse IgG (Alexa Fluor® 488, ab150113) used at 1/2000 dilution.
Negative control: HeLa.
Gated on viable cells.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab9509 尚未被引用在任何文献中。