重组Anti-Integrin beta 3抗体[ERP17507] (ab179473)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)](https://www.abcam.cn/ps/products/179/ab179473/Images/ab179473-260335-anti-integrin-beta-3-antibody-erp17507-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [ERP17507] to Integrin beta 3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Integrin beta 3抗体[ERP17507]
参阅全部 Integrin beta 3 一抗 -
描述
兔单克隆抗体[ERP17507] to Integrin beta 3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HT-29, HEK-293, HUVEC, HeLa and HEL 92.1.7 whole cell lysates; Human spleen lysate; Human plasma. IHC-P: Human spleen, osteosarcomas and thyroid cancer tissues. IP: HUVEC whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
ERP17507 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab179473于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000. Detects a band of approximately 130, 97 kDa (predicted molecular weight: 87 kDa).
|
| IHC-P |
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 130, 97 kDa (predicted molecular weight: 87 kDa). |
|
IHC-P
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
组织特异性
Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis. -
疾病相关
Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors. -
序列相似性
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
翻译后修饰
Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3690 Human
- Entrez Gene: 16416 Mouse
- Omim: 173470 Human
- SwissProt: P05106 Human
- SwissProt: O54890 Mouse
- Unigene: 218040 Human
- Unigene: 87150 Mouse
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别名
- BDPLT16 antibody
- BDPLT2 antibody
- CD 61 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Integrin beta 3 with ab179473 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Positive staining on platelets (PMID: 25356112) of Human spleen is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)](https://www.abcam.cn/ps/products/179/ab179473/Images/ab179473-341253-Ab179473.jpg)
Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)All lanes : Anti-Integrin beta 3 antibody [ERP17507] (ab179473) at 1/500 dilution
Lane 1 : U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
Lane 2 : HEL (Human Erythroleukemia erythroblast) whole cell lysates
Lane 3 : LnCaP (Human prostate carcinoma epithelial cell) whole cell lysates
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 5 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates
Lane 6 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
Lane 7 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 8 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 9 : Mouse brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsLnCaP, HeLa and PC-3 are low expression controls.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)](https://www.abcam.cn/ps/products/179/ab179473/Images/ab179473-260337-anti-integrin-beta-3-antibody-erp17507-western-blot.jpg)
Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)Anti-Integrin beta 3 antibody [ERP17507] (ab179473) at 1000 cells + HEL 92.1.7 (Human bone marrow erythroleukemia cell line) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 87 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with the description in the literature (PMID: 1710517).
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![Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)](https://www.abcam.cn/ps/products/179/ab179473/Images/ab179473-260336-anti-integrin-beta-3-antibody-erp17507-western-blot.jpg)
Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)Anti-Integrin beta 3 antibody [ERP17507] (ab179473) at 1/5000 dilution + Human plasma at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 87 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with the description in the literature (PMID: 1710517).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)](https://www.abcam.cn/ps/products/179/ab179473/Images/ab179473-260334-anti-integrin-beta-3-antibody-erp17507-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)Immunohistochemical analysis of paraffin-embedded Human osteosarcomas tissue labeling Integrin beta 3 with ab179473 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Membrane staining on cells of Human osteosarcomas is observed; several multinucleated osteoclasts are positively stained (PMID: 15692727; PMID: 25534583).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)](https://www.abcam.cn/ps/products/179/ab179473/Images/ab179473-260333-anti-integrin-beta-3-antibody-erp17507-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling Integrin beta 3 with ab179473 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Membrane or cytoplasmic staining on tumor cells of Human thyroid cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Integrin beta 3 antibody [ERP17507] (ab179473)](https://www.abcam.cn/ps/products/179/ab179473/Images/ab179473-12-benefits-of-recombinant-antibodies.png)
Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (11)
ab179473 被引用在 11 文献中.
- Wu M et al. HOXA10 Expressing UCMSCs Transplantation Improved Endometrial Receptivity on Endometrial Injury. Curr Stem Cell Res Ther 18:1001-1012 (2023). PubMed: 36121094
- Kato T et al. Interplay of adherens junctions and matrix proteolysis determines the invasive pattern and growth of squamous cell carcinoma. Elife 12:N/A (2023). PubMed: 36892272
- Tun X et al. Integrin β3-Mediated Cell Senescence Associates with Gut Inflammation and Intestinal Degeneration in Models of Alzheimer's Disease. Int J Mol Sci 24:N/A (2023). PubMed: 36982771
- Couto-Rodriguez A et al. Platelet-Derived Extracellular Vesicles as Lipid Carriers in Severe Allergic Inflammation. Int J Mol Sci 24:N/A (2023). PubMed: 37628895
- Närvä E et al. MASTL is enriched in cancerous and pluripotent stem cells and influences OCT1/OCT4 levels. iScience 25:104459 (2022). PubMed: 35677646