重组Anti-Integrin beta 3抗体[EPR2342] (ab119992)
![Western blot - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)](https://www.abcam.cn/ps/products/119/ab119992/Images/ab119992-504668-anti-Integrin-beta-3-antibody-epr2342-western-blot-human-mouse.jpg "Western blot - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2342] to Integrin beta 3
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-Integrin beta 3抗体[EPR2342]
参阅全部 Integrin beta 3 一抗 -
描述
兔单克隆抗体[EPR2342] to Integrin beta 3 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Flow Cyt (intra): HUVEC cells. IP: HUVEC whole cell lysate WB: U-87MG, HEL, HUVEC and C2C12 whole cell lysate.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2342 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
- Anti-Integrin beta 3 antibody [EPR2342] - BSA and Azide free (ab239991)
- Anti-Integrin beta 3 antibody [EPR2342] - Low endotoxin, Azide free (ab246708)
- Alexa Fluor® 488 Anti-Integrin beta 3 antibody [EPR2342] (ab320296)
- Alexa Fluor® 647 Anti-Integrin beta 3 antibody [EPR2342] (ab320297)
- PE Anti-Integrin beta 3 antibody [EPR2342] (ab320298)
- APC Anti-Integrin beta 3 antibody [EPR2342] (ab320299)
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119992于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/20.
|
|
| WB |
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 87 kDa).
|
|
| IP |
1/20.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/20. |
|
WB
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 87 kDa). |
|
IP
1/20. |
靶标
-
功能
Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
组织特异性
Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis. -
疾病相关
Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors. -
序列相似性
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
翻译后修饰
Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 3690 Human
- Entrez Gene: 16416 Mouse
- Entrez Gene: 29302 Rat
- Omim: 173470 Human
- SwissProt: P05106 Human
- SwissProt: O54890 Mouse
- Unigene: 218040 Human
- Unigene: 87150 Mouse
-
别名
- BDPLT16 antibody
- BDPLT2 antibody
- CD 61 antibody
see all
图片
-
Western blot - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)All lanes : Anti-Integrin beta 3 antibody [EPR2342] (ab119992) at 1/1000 dilution
Lane 1 : U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 2 : HEL (Human Erythroleukemia erythroblast) whole cell lysate
Lane 3 : LnCaP (Human prostate carcinoma epithelial cell) whole cell lysate
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate
Lane 6 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 7 : C2C12 (Mouse myoblasts myoblast) whole cell lysate
Lane 8 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 40 secondsBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used as a loading control.
LnCaP, HeLa and PC-3 are low expression cell lines ( PMID: 21867506, PMID: 25248000).
Though there are publications stating expression of Integrin beta 3 in RAW264.7, ab119992 and ab179473 show negative in Raw264.7. -
![Immunoprecipitation - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)](https://www.abcam.cn/ps/products/119/ab119992/Images/ab119992-5-anti-integrin-beta-3-antibody-epr2342-immunoprecipitation-huvec-human.jpg)
Immunoprecipitation - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)Purified ab119992 at 1:20 dilution (0.7µg) immunoprecipitating Integrin beta 3 in HUVEC whole cell lysate.
Lane 1 (input): HUVEC (Human umbilical vein endothelial cell) whole cell lysate 10µg.
Lane 2 (+): ab119992 + HUVEC whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab119992 in HUVEC whole cell lysate.
VeriBlot for IP Detection Reagent (HRP)(ab131366) (1:1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 110 kDa -
![Flow Cytometry (Intracellular) - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)](https://www.abcam.cn/ps/products/119/ab119992/Images/ab119992-311385-anti-integrin-beta-3-antibody-epr2342-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HUVEC (Human umbilical vein endothelial cell) cell line labeling Integrin beta 3 with ab119992 at 1/20 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
-
![Anti-Integrin beta 3 antibody [EPR2342] (ab119992)](https://www.abcam.cn/ps/products/119/ab119992/Images/ab119992-4-benefits-of-recombinant-antibodies.png)
Anti-Integrin beta 3 antibody [EPR2342] (ab119992)
数据表及文件
-
SDS download
-
Datasheet download
文献 (31)
ab119992 被引用在 31 文献中.
- Huang L et al. Inhibition of Integrin αvβ3-FAK-MAPK signaling constrains the invasion of T-ALL cells. Cell Adh Migr 17:1-14 (2023). PubMed: 36944577
- Geng J et al. LncRNA NEAT1 affects endometrial receptivity by regulating HOXA10 promoter activity. Cell Cycle 21:1932-1944 (2022). PubMed: 35574918
- Zhao J et al. Inhibition of post-surgery tumour recurrence via a sprayable chemo-immunotherapy gel releasing PD-L1 antibody and platelet-derived small EVs. J Nanobiotechnology 20:62 (2022). PubMed: 35109878
- Yang B et al. γ-Secretase inhibitors suppress IL-20-mediated osteoclastogenesis via Notch signalling and are affected by Notch2 in vitro. Scand J Immunol 96:e13169 (2022). PubMed: 35384009
- Zhao M et al. Cancer-Associated Fibroblast-Derived Exosomal miRNA-320a Promotes Macrophage M2 Polarization In Vitro by Regulating PTEN/PI3Kγ Signaling in Pancreatic Cancer. J Oncol 2022:9514697 (2022). PubMed: 35813857