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Neuroscience Cell Type Marker Neural Stem Cell marker

Anti-Integrin beta 1 (phospho T788 + T789)抗体(ab5189)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (9)

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Western blot - Anti-Integrin beta 1 (phospho T788 + T789) antibody (ab5189)
  • Immunocytochemistry - Anti-Integrin beta 1 (phospho T788 + T789) antibody (ab5189)

Key features and details

  • Rabbit polyclonal to Integrin beta 1 (phospho T788 + T789)
  • Suitable for: ICC, WB
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Integrin beta 1 (phospho T788 + T789)抗体
    参阅全部 Integrin beta 1 一抗
  • 描述

    兔多克隆抗体to Integrin beta 1 (phospho T788 + T789)
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC, WBmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Chicken
  • 免疫原

    Synthetic peptide corresponding to Human Integrin beta 1 (phospho T788 + T789).
    Database link: P05558

  • 阳性对照

    • WB: HeLa cells in mitosis. ICC: THP-1 cells.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that isb reactive with non-phosphorylated Integrin beta 1 receptor protein. The final product is generated by affinity chromatography using an Integrin receptor-derived peptide that is phosphorylated at threonine 788 and threonine 789.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Beta
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Embryonic Stem Cells
    • Surface Molecules
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Intracellular

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Integrin beta 1 protein (ab114157)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5189于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
1/250.
WB (2)
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 88 kDa.
说明
ICC
1/250.
WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 88 kDa.

靶标

  • 功能

    Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881).
    Isoform 5: Isoform 5 displaces isoform 1 in striated muscles.
    (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for human echoviruses 1 and 8 (PubMed:8411387). Acts as a receptor for cytomegalovirus/HHV-5 (PubMed:20660204). Acts as a receptor for Epstein-Barr virus/HHV-4 (PubMed:17945327). Integrin ITGA5:ITGB1 acts as a receptor for human parvovirus B19 (PubMed:12907437). Integrin ITGA2:ITGB1 acts as a receptor for human rotavirus (PubMed:12941907). Acts as a receptor for mammalian reovirus (PubMed:16501085). In case of HIV-1 infection, integrin ITGA5:ITGB1 binding to extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions (PubMed:10397733).
  • 组织特异性

    Isoform 1 is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4, rather than isoform 3, is selectively expressed in peripheral T-cells. Isoform 3 is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle).
  • 序列相似性

    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • 翻译后修饰

    The cysteine residues are involved in intrachain disulfide bonds.
  • 细胞定位

    Cell membrane, sarcolemma. Cell junction. In cardiac muscle, isoform 5 is found in costameres and intercalated disks and Cell membrane. Cell projection, invadopodium membrane. Cell projection, ruffle membrane. Recycling endosome. Melanosome. Cleavage furrow. Cell projection, lamellipodium. Cell projection, ruffle. Cell junction, focal adhesion. Cell surface. Isoform 2 does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in a few intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid-telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow. In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at the base of the lamellipodia formed by the separating daughter cells. Colocalizes with ITGB1BP1 and metastatic suppressor protein NME2 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen. Translocates from peripheral focal adhesions sites to fibrillar adhesions in a ITGB1BP1-dependent manner. Enriched preferentially at invadopodia, cell membrane protrusions that correspond to sites of cell invasion, in a collagen-dependent manner. Localized at plasma and ruffle membranes in a collagen-independent manner.
  • Target information above from: UniProt accession P05556 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 374058 Chicken
    • Entrez Gene: 3688 Human
    • Entrez Gene: 16412 Mouse
    • Entrez Gene: 24511 Rat
    • Omim: 135630 Human
    • SwissProt: P07228 Chicken
    • SwissProt: P05556 Human
    • SwissProt: P09055 Mouse
    • SwissProt: P49134 Rat
    • Unigene: 643813 Human
    • Unigene: 263396 Mouse
    • Unigene: 25733 Rat
    see all
  • 别名

    • beta1 integrin antibody
    • CD_antigen=CD29 antibody
    • CD29 antibody
    • Fibronectin receptor subunit beta antibody
    • FNRB antibody
    • Glycoprotein IIa antibody
    • GP IIa antibody
    • GPIIA antibody
    • Integrin beta-1 antibody
    • Integrin subunit beta 1 antibody
    • integrin VLA-4 beta subunit antibody
    • Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) antibody
    • ITB1_HUMAN antibody
    • ITGB1 antibody
    • MDF2 antibody
    • MSK12 antibody
    • OTTHUMP00000019420 antibody
    • very late activation protein antibody
    • Very late activation protein, beta polypeptide antibody
    • VLA BETA antibody
    • VLA-4 subunit beta antibody
    • VLA-BETA antibody
    • VLAB antibody
    • VLAbeta antibody
    see all

图片

  • Western blot - Anti-Integrin beta 1 (phospho T788 + T789) antibody (ab5189)
    Western blot - Anti-Integrin beta 1 (phospho T788 + T789) antibody (ab5189)
    Peptide Competition:  HeLa extracts of mitotic cells generated by treatment with taxol, were resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins then were transferred to nitrocellulose and incubated with 0.50 ab5189 antibody, following prior incubation with: (1) the phosphopeptide immunogen, (2) the non-phosphorylated peptide corresponding to the phosphopeptide, (3) a generic phosphothreonine-containing peptide, and (4) no peptide. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, demonstrating the specificity of the Integrin beta 1 receptor antibody for this phosphorylated residue. Peptide Competition: HeLa extracts of mitotic cells generated by treatment with taxol, were resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins then were transferred to nit
  • Immunocytochemistry - Anti-Integrin beta 1 (phospho T788 + T789) antibody (ab5189)
    Immunocytochemistry - Anti-Integrin beta 1 (phospho T788 + T789) antibody (ab5189)

    THP-1 cells stained for Integrin beta 1 (green) using ab5189 at 1/250 dilution in ICC/IF. Followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300 dilution. Panel d represents the merged image showing localization in the membrane. Panel e is untreated cell with less signal. Panel f represents control cells with no primary antibody to assess background.

实验方案

  • Peptide Competition Experiment
  • Western blotting protocol

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (9)

发表研究结果有使用 ab5189?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab5189 被引用在 9 文献中.

  • Böttcher RT  et al. New insights into the phosphorylation of the threonine motif of the β1 integrin cytoplasmic domain. Life Sci Alliance 5:N/A (2022). PubMed: 34996844
  • Li Y  et al. Controlling the Multiscale Network Structure of Fibers To Stimulate Wound Matrix Rebuilding by Fibroblast Differentiation. ACS Appl Mater Interfaces 11:28377-28386 (2019). PubMed: 31251577
  • Demiray YE  et al. Ndr2 Kinase Controls Neurite Outgrowth and Dendritic Branching Through a1 Integrin Expression. Front Mol Neurosci 11:66 (2018). PubMed: 29559888
  • Peng X  et al. Oxyfadichalcone C inhibits melanoma A375 cell proliferation and metastasis via suppressing PI3K/Akt and MAPK/ERK pathways. Life Sci 206:35-44 (2018). PubMed: 29782872
  • Csoboz B  et al. Chemotherapy induced PRL3 expression promotes cancer growth via plasma membrane remodeling and specific alterations of caveolae-associated signaling. Cell Commun Signal 16:51 (2018). PubMed: 30157875
  • Jongbloets BC  et al. Stage-specific functions of Semaphorin7A during adult hippocampal neurogenesis rely on distinct receptors. Nat Commun 8:14666 (2017). WB ; Mouse . PubMed: 28281529
  • Parkash J  et al. Semaphorin7A regulates neuroglial plasticity in the adult hypothalamic median eminence. Nat Commun 6:6385 (2015). WB . PubMed: 25721933
  • Rehberg K  et al. The serine/threonine kinase Ndr2 controls integrin trafficking and integrin-dependent neurite growth. J Neurosci 34:5342-54 (2014). PubMed: 24719112
  • Oulidi A  et al. TRPV2 mediates adrenomedullin stimulation of prostate and urothelial cancer cell adhesion, migration and invasion. PLoS One 8:e64885 (2013). WB ; Human . PubMed: 23741410

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Western blot abreview for Anti-Integrin beta 1 (phospho T788 + T789) antibody

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Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH3t3 Fibroblasts)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
40 µg
Specification
NIH3t3 Fibroblasts
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Jun 21 2018

Western blot abreview for Anti-Integrin beta 1 (phospho T788 + T789) antibody

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Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Lung)
Gel Running Conditions
Reduced Denaturing (5-15%)
Loading amount
30 µg
Specification
Lung
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Apr 24 2017

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