为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    按产品类型
    蛋白质与肽
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Neuroscience Cell Type Marker Neural Stem Cell marker

Anti-Integrin beta 1抗体[BV7] (ab7168)

  • Datasheet
Reviews (1)Q&A (16)References (16)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Flow Cytometry (Intracellular) - Anti-Integrin beta 1 antibody [BV7] (ab7168)
  • Flow Cytometry (Intracellular) - Anti-Integrin beta 1 antibody [BV7] (ab7168)

Key features and details

  • Mouse monoclonal [BV7] to Integrin beta 1
  • Suitable for: Flow Cyt (Intra)
  • Reacts with: Human
  • Isotype: IgG1

选择批间可重复性更高的重组抗体

Product image
Anti-Integrin beta 1 antibody [EPR1040Y] (ab134179)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Integrin beta 1抗体[BV7]
    参阅全部 Integrin beta 1 一抗
  • 描述

    小鼠单克隆抗体[BV7] to Integrin beta 1
  • 宿主

    Mouse
  • 特异性

    This monoclonal antibody binds to human beta 1 integrin chain present on tumor cells. The antibody is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells, as well as several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC-1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells.
  • 经测试应用

    适用于: Flow Cyt (Intra)more details
  • 种属反应性

    与反应: Human
  • 免疫原

    Tissue, cells or virus corresponding to Human Integrin beta 1. Human umbilical vein EC

  • 表位

    Antibody BV7 stains the extracellular domain of beta-1 integrin.
  • 阳性对照

    • HT-29 colon carcinoma, Endothelial cells. Flow Cyt (Intra): HUVEC cells; MCF-7 cells
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7.60
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    BV7
  • 同种型

    IgG1
  • 研究领域

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Beta
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Embryonic Stem Cells
    • Surface Molecules
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Intracellular

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human Integrin beta 1 protein (ab114157)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7168于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use 1µg for 106 cells.

(paraformaldehyde or methanol fixed cells).

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

说明
Flow Cyt (Intra)
Use 1µg for 106 cells.

(paraformaldehyde or methanol fixed cells).

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881).
    Isoform 5: Isoform 5 displaces isoform 1 in striated muscles.
    (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for human echoviruses 1 and 8 (PubMed:8411387). Acts as a receptor for cytomegalovirus/HHV-5 (PubMed:20660204). Acts as a receptor for Epstein-Barr virus/HHV-4 (PubMed:17945327). Integrin ITGA5:ITGB1 acts as a receptor for human parvovirus B19 (PubMed:12907437). Integrin ITGA2:ITGB1 acts as a receptor for human rotavirus (PubMed:12941907). Acts as a receptor for mammalian reovirus (PubMed:16501085). In case of HIV-1 infection, integrin ITGA5:ITGB1 binding to extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions (PubMed:10397733).
  • 组织特异性

    Isoform 1 is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4, rather than isoform 3, is selectively expressed in peripheral T-cells. Isoform 3 is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle).
  • 序列相似性

    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • 翻译后修饰

    The cysteine residues are involved in intrachain disulfide bonds.
  • 细胞定位

    Cell membrane, sarcolemma. Cell junction. In cardiac muscle, isoform 5 is found in costameres and intercalated disks and Cell membrane. Cell projection, invadopodium membrane. Cell projection, ruffle membrane. Recycling endosome. Melanosome. Cleavage furrow. Cell projection, lamellipodium. Cell projection, ruffle. Cell junction, focal adhesion. Cell surface. Isoform 2 does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in a few intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid-telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow. In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at the base of the lamellipodia formed by the separating daughter cells. Colocalizes with ITGB1BP1 and metastatic suppressor protein NME2 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen. Translocates from peripheral focal adhesions sites to fibrillar adhesions in a ITGB1BP1-dependent manner. Enriched preferentially at invadopodia, cell membrane protrusions that correspond to sites of cell invasion, in a collagen-dependent manner. Localized at plasma and ruffle membranes in a collagen-independent manner.
  • Target information above from: UniProt accession P05556 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3688 Human
    • Omim: 135630 Human
    • SwissProt: P05556 Human
    • Unigene: 643813 Human
    • 别名

      • beta1 integrin antibody
      • CD_antigen=CD29 antibody
      • CD29 antibody
      • Fibronectin receptor subunit beta antibody
      • FNRB antibody
      • Glycoprotein IIa antibody
      • GP IIa antibody
      • GPIIA antibody
      • Integrin beta-1 antibody
      • Integrin subunit beta 1 antibody
      • integrin VLA-4 beta subunit antibody
      • Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) antibody
      • ITB1_HUMAN antibody
      • ITGB1 antibody
      • MDF2 antibody
      • MSK12 antibody
      • OTTHUMP00000019420 antibody
      • very late activation protein antibody
      • Very late activation protein, beta polypeptide antibody
      • VLA BETA antibody
      • VLA-4 subunit beta antibody
      • VLA-BETA antibody
      • VLAB antibody
      • VLAbeta antibody
      see all

    图片

    • Flow Cytometry (Intracellular) - Anti-Integrin beta 1 antibody [BV7] (ab7168)
      Flow Cytometry (Intracellular) - Anti-Integrin beta 1 antibody [BV7] (ab7168)

      Overlay histogram showing MCF-7 cells stained with ab7168 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7168, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.

      Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    • Flow Cytometry (Intracellular) - Anti-Integrin beta 1 antibody [BV7] (ab7168)
      Flow Cytometry (Intracellular) - Anti-Integrin beta 1 antibody [BV7] (ab7168)
      Overlay histogram showing HUVEC cells stained with ab7168 (blue line). Red line was isotype control mouse IgG1. Black line was an irrelevant mouse IgG1 used under the same conditions. Controls and anti-human ß1-integrin at 0.4 µg/100000 cells (1 hr 4°C).

    实验方案

    • Flow cytometry protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (16)

    发表研究结果有使用 ab7168?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab7168 被引用在 16 文献中.

    • Anguiano M  et al. The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion. PLoS One 15:e0220019 (2020). PubMed: 31945053
    • Fujita M  et al. Autocrine Production of PDGF Stimulated by the Tenascin-C-Derived Peptide TNIIIA2 Induces Hyper-Proliferation in Glioblastoma Cells. Int J Mol Sci 20:N/A (2019). PubMed: 31261783
    • Anguiano M  et al. Characterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis. PLoS One 12:e0171417 (2017). PubMed: 28166248
    • Jahangiri A  et al. Cross-activating c-Met/ß1 integrin complex drives metastasis and invasive resistance in cancer. Proc Natl Acad Sci U S A 114:E8685-E8694 (2017). PubMed: 28973887
    • Perico ME  et al. Prostate-specific membrane antigen (PSMA) assembles a macromolecular complex regulating growth and survival of prostate cancer cells "in vitro" and correlating with progression "in vivo". Oncotarget 7:74189-74202 (2016). PubMed: 27713116
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 17 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-Integrin beta 1 antibody [BV7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (OVCAR5 cell line)
    Total protein in input
    50 µg
    Specification
    OVCAR5 cell line
    Immuno-precipitation step
    Protein A/G
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. John Andersen

    Verified customer

    提交于 Jul 21 2006

    Question

    I would like to immunoprecipitate beta1 integrin from mouse and human cells. In some cases I want to biotinylate the surface proteins prior to preparing a cellular lysate and immunoprecipitation.
    Can you suggest an antibody that would be useful?
    Thank you,

    Read More

    Abcam community

    Verified customer

    Asked on Feb 20 2012

    Answer

    Thank you for contacting us.
    We would recommend following antibodies for IP of integrin beta 1.
    ab24693, can be used for IP of both human and mouse protein.
    ab7168, ab30388, ab77803, and ab78502 can be used for IPing human beta 1 integrin protein and is fully guaranteed, we however haven't tested it with mouse protein so we are unsure about mouse cross reactivity. If you are interested in using one of these with mouse protein you may be interested in Abreview discount programme. Click the below link for more info;
    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount
    ab95623 is used in IP with mouse lysates only and it is eligible for human IP collaboration discount.
    Checkout the secondary antibody combinations;
    ab7168 - ab96879
    ab30388 - ab79091
    ab95623- ab106783
    For full list of secondary's click the link; https://www.abcam.com/index.html?pageconfig=productmap&cl=918
    We do not have reagents for cell prtoein biotinylation. I can suggest the Invitrogen website;
    http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Biotin-Derivatives-and-Haptens/Biotinylation-and-Haptenylation-Reagents.html
    If you are interested in Biotin conjugated antibodies then we have various biotinylation kits available. These are; https://www.abcam.com/index.html?pageconfig=resource&rid=13148

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Feb 20 2012

    Question

    We use homogenized human brain tissue to isolate the protein.  We run the protein on 4-20% gels. We used PVDF membrane for the transfer.  We use licor-odyssey blocking buffer as our blocking agent for 1 hour at room temperature.  We used a concentration of 1:1000 for all primary antibodies.  We incubated in primary antibody overnight at 4 degrees C. We washed 3 times for 10 minutes in PBS-Tween then incubated in Goat-anti-mouse secondary antibody from licor-odyssey for 1 hour at room temperature.  We then washed 3 times for 10 minutes  with PBS-Tween and used a Licor-Odyssey Infrared Imager to detect the proteins.   I think we would like credits issued for the Integrin beta 1 and 3.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 07 2011

    Answer

    Thank you very much for sending this information. I am sorry that these antibodies did not work as expected. I have issued the credits to Cedarlane, and I will contact them to pass these credits on to you. Please let me know if you have any questions or if there is anything else that we can do for you.

    Read More

    Abcam Scientific Support

    回复于 Dec 07 2011

    Question

    Attached are Images from the antibodies on Human samples.  

    Read More

    Abcam community

    Verified customer

    Asked on Dec 06 2011

    Answer

    Thanks so much for forwarding the images. I am sorry to see that the results were so poor. Could you also send the general protocol that was used? As mentioned previously, I can send replacements or issue credit or refunds for the integrin beta 1 and integrin beta 3 antibodies, and I can issue a discount code to submit with an Abreview of the MOBP antibody in exchange for a free primary. We have several other antibodies to these targets that have been tested in human Western blot if you are interested in replacements. For integrin beta 1, our top-rated antibody is the mouse monoclonal ab30394 and our top-rate integrin beta 3 antibody is the mouse monoclonal ab7167- https://www.abcam.com/Integrin-beta-1-antibody-12G10-ab30394.html https://www.abcam.com/Integrin-beta-3-antibody-BV4-ab7167.html If you are interested in any of these options, please send me your Cedarlane customer code and full address so that I can make sure the replacement or refund is directed to you. Please let me know if you have any questions or if there is anything else that we can do for you.

    Read More

    Abcam Scientific Support

    回复于 Dec 06 2011

    Question

    Thanks for the quick reply. There were no bands with those antibodies which is something that we rarely find. We ran the blots with other antibodies (psd95) and got the typical bands.   

    Read More

    Abcam community

    Verified customer

    Asked on Dec 02 2011

    Answer

    Thanks for your reply and for clarifying that, and I look forward to receiving any data or images that you can provide. Please let me know if you need anything else from me.

    Read More

    Abcam Scientific Support

    回复于 Dec 02 2011

    Question

    We ordered 3 antibodies.  They were MOBP AB66253, Integrin beta 1 AB7168 and Integrin beta 3 AB110131. We used western blot on Human protein samples, Rat protein samples, rabbit protein samples and cat protein samples. We did not see any bands in either homogenate or synaptoneurosome preparations.   We ordered these antibodies through cedarlane  

    Read More

    Abcam community

    Verified customer

    Asked on Nov 30 2011

    Answer

    Thank you very much for your replies and for sending the information. I am sorry to hear that none of these antibodies gave the expected results. Just to clarify, there were no bands in Western blot even with the human protein samples? We do guarantee the integrin beta 1 antibody ab7168 and the integrin beta 3 antibody ab110131 to work in human samples, though the MOBP antibody ab66253 has not been tested with endogenous human samples. Could you also send some details about the Western blot protocol that was used, for our documentation? I would be happy to see if there are any suggestions that might improve the results. For ab7168 and ab110131, I can send replacement antibodies or issue credits if you would prefer. Could you also please send your Cedarlane customer number, so that I can ensure they pass on any credit or replacement to you? Thank you for your time, and I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

    Read More

    Abcam Scientific Support

    回复于 Nov 30 2011

    Question

    I had a customer contact me regarding some problems she had with three antibodies ordered on PO 134004. I have attached her protocol information and images. She had also ordered AB62631 at the same time, and this antibody worked well for her.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 08 2011

    Answer

    Thank you for your inquiry and I'm sorry to hear that your customer is having trouble with these products. As for ab66253, we haven't tested this antibody in endogenous samples, so we are unsure how it will work with brain tissue.  However, we have tested both ab7168 and ab110131 to work with endogenous samples. What species were the brain tissues from?   Even the loading control seems a bit weak.  They may consider loading more protein than they currently are. We have heard that some customers have difficulty with our antibodies when using LiCor detection.  Does the customer have the ability to run it again using chromogenic/HRP detection? Typically what we've heard is that the Odyssey blocking buffer may inhibit binding.  If the customer can try blocking with 5% milk or BSA or 0.5% casein for 1 hr at RT instead of using the system's buffer, I would definitely recommend they try it. Also, they should decrease the time of washes to 3 x 5 mins instead of 10 mins. I hope this information helps.  Please contact us with any other questions or if the protocol suggestions do not improve the results.

    Read More

    Abcam Scientific Support

    回复于 Nov 08 2011

    Question

    Dear technical team,   Our customer would like to purchase anti-integrin beta 1 antiody for inhibition on mouse sample.   There's no product for mouse sample so, I would like to know it is possible to test with discount code.   Check this please.  

    Read More

    Abcam community

    Verified customer

    Asked on Sep 30 2011

    Answer

    There is 92.5% homology between the Mouse and the Human integrin beta 1. The antibody ab7168 should therefore work with Mouse samples. DISCOUNT CODE: XXXXXXXXXX Expiration date: DD MM YYYY I am very pleased to hear your customer would like to accept our offer and test ab7168 in Mouse. This code will give your customer 1 free primary antibody before the expiration date. To redeem this offer, please ask your customer to submit an Abreview for Mouse and to include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that customers can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    回复于 Sep 30 2011

    Question

    Customer would like to know: what end of the integrin was the antibody raised against?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 07 2004

    Answer

    Thank you for your phone call. I regret to inform you that concerning the antibodies that you are interested in, we do not have any information regarding the epitopes. If you have any more questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Apr 14 2004

    Question

    What do you recommend as a positive control?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 30 2004

    Answer

    Since ab7168 is active on HT-29 colon carcinoma cells, HT 29 whole cell lysate (ab3952) may be a good choice.

    Read More

    Abcam Scientific Support

    回复于 Jan 30 2004

    1-10 of 17 Abreviews or Q&A

    •  向后
    • 1
    • 2
    • 向前 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2025 艾博抗(上海)贸易有限公司版权所有