Anti-Integrin alpha V抗体[P2W7] (ab11470)
![Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V antibody [P2W7] (ab11470)](https://www.abcam.cn/ps/products/11/ab11470/Images/ab11470-18993-anti-integrin-alpha-v-antibody-p2w7-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V antibody [P2W7] (ab11470)")
Key features and details
- Mouse monoclonal [P2W7] to Integrin alpha V
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Integrin alpha V抗体[P2W7]
参阅全部 Integrin alpha V 一抗 -
描述
小鼠单克隆抗体[P2W7] to Integrin alpha V -
宿主
Mouse -
经测试应用
适用于: ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human Integrin alpha V. Ocular melanoma cell line (V+B2) a clone expressing high levels of alpha-v, beta-1
Database link: P06756 -
阳性对照
- Tested with squamous cell carcinoma cells. A375M melanoma cell line. A549 cell line IF/ICC
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常规说明
Human alpha v is about 150-160kDa under non-reducing conditions. Under reducing conditions it is cleaved into 2 bands of about 125-130kDa +20-25kDa.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
The antibody is Protein A purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
P2W7 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11470于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| Flow Cyt |
Use a concentration of 1 - 10 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use a concentration of 1 - 10 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3685 Human
- Omim: 193210 Human
- SwissProt: P06756 Human
- Unigene: 436873 Human
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别名
- antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD51 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V antibody [P2W7] (ab11470)
ICC/IF image of ab11470 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11470, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, a goat anti-mouse DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Flow Cytometry - Anti-Integrin alpha V antibody [P2W7] (ab11470)](https://www.abcam.cn/ps/products/11/ab11470/Images/ab11470-18992-anti-integrin-alpha-v-antibody-p2w7-flow-cytometry.jpg)
Flow Cytometry - Anti-Integrin alpha V antibody [P2W7] (ab11470)Overlay histogram showing A549 cells stained with ab11470 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11470, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed A549 cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab11470 被引用在 7 文献中.
- Tang YSC et al. Identification, Characterization, and Optimization of Integrin αvβ₆-Targeting Peptides from a One-Bead One-Compound (OBOC) Library: Towards the Development of Positron Emission Tomography (PET) Imaging Agents. Molecules 24:N/A (2019). PubMed: 30654483
- Zhang C et al. Photoacoustic and Fluorescence Imaging of Cutaneous Squamous Cell Carcinoma in Living Subjects Using a Probe Targeting Integrin αvβ6. Sci Rep 7:42442 (2017). PubMed: 28181579
- Megiorni F et al. Pharmacological targeting of the ephrin receptor kinase signalling by GLPG1790 in vitro and in vivo reverts oncophenotype, induces myogenic differentiation and radiosensitizes embryonal rhabdomyosarcoma cells. J Hematol Oncol 10:161 (2017). WB ; Human . PubMed: 28985758
- Ramanathan S et al. Thrombospondin-1 and angiotensin II inhibit soluble guanylyl cyclase through an increase in intracellular calcium concentration. Biochemistry 50:7787-99 (2011). PubMed: 21823650
- Farsinejad A et al. Classification of Iranian patients with Glanzmann's Thrombasthenia using a flow cytometric method. Platelets 22:321-7 (2011). PubMed: 21526886
- Wang C et al. transient scrotal hyperthermia and levonorgestrel enhance testosterone-induced spermatogenesis suppression in men through increased germ cell apoptosis. J Clin Endocrinol Metab 92:3292-304 (2007). PubMed: 17504903
- von Schlippe M et al. Functional interaction between E-cadherin and alphav-containing integrins in carcinoma cells. J Cell Sci 113 ( Pt 3):425-37 (2000). PubMed: 10639330