AB11470

Anti-Integrin alpha V抗体[P2W7]

Name: Integrin alpha V抗体[P2W7] (ab11470)| Abcam中文官网
Brand: Abcam Limited
SKU: AB11470
Price: 5019 CNY
Availability: InStock

Anti-Integrin alpha V antibody [P2W7]

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(8 Publications)

Mouse Monoclonal Integrin alpha V antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 8 publications.

查看别名

CD51, MSK8, VNRA, VTNR, ITGAV, Integrin alpha-V, Vitronectin receptor, Vitronectin receptor subunit alpha

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V antibody [P2W7] (AB11470)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V antibody [P2W7] (AB11470)

ICC/IF image of ab11470 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11470, 5μg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, a goat anti-mouse DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Flow Cytometry - Anti-Integrin alpha V antibody [P2W7] (AB11470)

Flow Cyt

Unknown

Flow Cytometry - Anti-Integrin alpha V antibody [P2W7] (AB11470)

Overlay histogram showing A549 cells stained with ab11470 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11470, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was a Goat Anti-Mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed A549 cells used under the same conditions.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

P2W7

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

Flow Cyt, ICC/IF

applications

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-10 µg/mL", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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产品详情

Human alpha v is about 150-160kDa under non-reducing conditions. Under reducing conditions it is cleaved into 2 bands of about 125-130kDa +20-25kDa.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

纯化说明

The antibody is Protein A purified from tissue culture supernatant.

存储溶液

Preservative: 0.02% Sodium azide Constituents: PBS

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha V also known as integrin av CD51 and CD51/CD61 is a transmembrane protein with an approximate mass of 125 kDa. It functions mechanically by mediating cell adhesion and signaling through its interaction with the extracellular matrix and other cells. Integrin alpha V is widely expressed in a variety of tissues including endothelial and epithelial cells as well as some tumor cells. This protein forms heterodimers typically with beta subunits like CD61 which is also referred to as beta 3 integrin to execute its biological functions.

Biological function summary

Integrin alpha V plays a pivotal role in cellular processes such as migration proliferation and survival. It participates in the formation of multi-molecular complexes within cellular structures. The integrin av integrins particularly in conjunction with its CD51/CD61 form contribute to the regulation of angiogenesis immune response and wound healing. These functions arise from the integrin’s ability to act as a receptor for numerous ligands including fibrinogen fibronectin and vitronectin.

Pathways

Integrin alpha V is a critical component of the integrin signaling and focal adhesion pathways. These pathways involve a network of interactions that include proteins like focal adhesion kinase (FAK) and Src family kinases. Integrin alpha V’s interaction within these pathways assists in transmitting mechanical signals from the extracellular environment to the intracellular signaling cascades affecting cytoskeleton dynamics and gene expression. The pathway interactions with integrin av also coordinate complex signaling with other receptors and matrix proteins like laminins enhancing cellular communication and response to environmental changes.

Integrin alpha V’s dysregulation associates with conditions such as cancer and fibrosis. Overexpression or aberrant functioning of integrin av contributes to tumor progression and metastasis by promoting angiogenesis and cell migration. In fibrotic diseases the protein's interactions with the extracellular matrix components are altered leading to excessive tissue scarring and dysfunction. Furthermore integrin alpha V interplays with proteins such as matrix metalloproteinases (MMPs) in the tumor microenvironment facilitating tissue remodeling and cancer cell invasion.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV : ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415). ITGAV : ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGAV : ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed : 18441324). ITGAV : ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed : 28302677). ITGAV : ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 19578119). ITGAV : ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464). ITGAV : ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed : 29030430). ITGAV : ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGAV : ITGB3 and ITGAV : ITGB6 act as receptors for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV : ITGB6 or ITGAV : ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed : 15184403, PubMed : 22278742, PubMed : 28117447). ITGAV : ITGB3 acts as a receptor for CD40LG (PubMed : 31331973). ITGAV : ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed : 10640428).. (Microbial infection) Integrin ITGAV : ITGB5 acts as a receptor for Adenovirus type C.. (Microbial infection) Integrin ITGAV : ITGB5 and ITGAV : ITGB3 act as receptors for Coxsackievirus A9 and B1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Herpes virus 8/HHV-8.. (Microbial infection) Integrin ITGAV : ITGB6 acts as a receptor for herpes simplex 1/HHV-1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Human parechovirus 1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for West nile virus.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

See full target information ITGAV

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 97:e0077023 PubMed37823645

2023

Efficient clathrin-mediated entry of enteric adenoviruses in human duodenal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Miriam Becker,Dario Valter Conca,Noemi Dorma,Nitesh Mistry,Elin Hahlin,Lars Frängsmyr,Marta Bally,Niklas Arnberg,Gisa Gerold

Molecules (Basel, Switzerland) 24: PubMed30654483

2019

Identification, Characterization, and Optimization of Integrin αβ₆-Targeting Peptides from a One-Bead One-Compound (OBOC) Library: Towards the Development of Positron Emission Tomography (PET) Imaging Agents.

Applications

Unspecified application

Species

Unspecified reactive species

Yng Sarah C Tang,Ryan A Davis,Tanushree Ganguly,Julie L Sutcliffe

Journal of hematology & oncology 10:161 PubMed28985758

2017

Pharmacological targeting of the ephrin receptor kinase signalling by GLPG1790 in vitro and in vivo reverts oncophenotype, induces myogenic differentiation and radiosensitizes embryonal rhabdomyosarcoma cells.

Applications

Species

Human

Francesca Megiorni,Giovanni Luca Gravina,Simona Camero,Simona Ceccarelli,Andrea Del Fattore,Vincenzo Desiderio,Federica Papaccio,Heather P McDowell,Rajeev Shukla,Antonio Pizzuti,Filip Beirinckx,Philippe Pujuguet,Laurent Saniere,Ellen Van der Aar,Roberto Maggio,Francesca De Felice,Cinzia Marchese,Carlo Dominici,Vincenzo Tombolini,Claudio Festuccia,Francesco Marampon

Scientific reports 7:42442 PubMed28181579

2017

Photoacoustic and Fluorescence Imaging of Cutaneous Squamous Cell Carcinoma in Living Subjects Using a Probe Targeting Integrin αβ.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Zhang,Yong Zhang,Kai Hong,Shu Zhu,Jie Wan

Biochemistry 50:7787-99 PubMed21823650

2011

Thrombospondin-1 and angiotensin II inhibit soluble guanylyl cyclase through an increase in intracellular calcium concentration.

Applications

Unspecified application

Species

Unspecified reactive species

Saumya Ramanathan,Stacy Mazzalupo,Scott Boitano,William R Montfort

Platelets 22:321-7 PubMed21526886

2011

Classification of Iranian patients with Glanzmann's Thrombasthenia using a flow cytometric method.

Applications

Unspecified application

Species

Unspecified reactive species

A Farsinejad,H Abolghasemi,A Kazemi,M Aghaiipour,E Hadjati,M Faranoush,M Jazebi,F Ala

The Journal of clinical endocrinology and metaboli 92:3292-304 PubMed17504903

2007

transient scrotal hyperthermia and levonorgestrel enhance testosterone-induced spermatogenesis suppression in men through increased germ cell apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Christina Wang,Yu-Gui Cui,Xing-Hai Wang,Yue Jia,Amiya Sinha Hikim,Yan-He Lue,Jian-Son Tong,Li-Xin Qian,Jia-Hao Sha,Zuo-Min Zhou,Laura Hull,Andrew Leung,Ronald S Swerdloff

Journal of cell science 113 ( Pt 3):425-37 PubMed10639330

2000

Functional interaction between E-cadherin and alphav-containing integrins in carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

M von Schlippe,J F Marshall,P Perry,M Stone,A J Zhu,I R Hart

View all publications

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Anti-Integrin alpha V抗体[P2W7]

Anti-Integrin alpha V antibody [P2W7]

Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V antibody [P2W7] (AB11470)

Flow Cytometry - Anti-Integrin alpha V antibody [P2W7] (AB11470)