Name: Anti-Integrin alpha V antibody [EPR16800]
Brand: RabMAb
SKU: ab179475
Rating: 5 (10 reviews)

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Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16800] to Integrin alpha V
Suitable for: WB, IP, ICC/IF, IHC-P
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP PE

产品名称

Anti-Integrin alpha V抗体[EPR16800]
参阅全部 Integrin alpha V 一抗
描述

兔单克隆抗体[EPR16800] to Integrin alpha V
宿主

Rabbit
经测试应用

适用于: WB, IP, ICC/IF, IHC-Pmore details
种属反应性

与反应: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: HUVEC, HT-29, A549, C6 and NIH/3T3 whole cell lysates; Human fetal kidney and fetal brain lysates; Mouse brain, kidney and spleen lysates; Rat brain and kidney lysates. IHC: Human kidney, human transitional cell carcinoma of bladder and mouse kidney tissues. ICC/IF: A549 cells. IP: A549 whole cell extract.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR16800
同种型

IgG
研究领域

The Abpromise guarantee

Abpromise™承诺保证使用ab179475于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB	(4)	1/5000. Detects a band of approximately 125, 135 kDa (predicted molecular weight: 116 kDa).
IP		1/40.
ICC/IF	(3)	1/500.
IHC-P	(3)	1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

说明
WB 1/5000. Detects a band of approximately 125, 135 kDa (predicted molecular weight: 116 kDa).
IP 1/40.
ICC/IF 1/500.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

功能

The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
序列相似性

Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
细胞定位

Membrane.
Target information above from: UniProt accession P06756 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 3685 Human
- Entrez Gene: 16410 Mouse
- Entrez Gene: 296456 Rat
- Omim: 193210 Human
- SwissProt: P06756 Human
- SwissProt: P43406 Mouse
- Unigene: 436873 Human
- Unigene: 227 Mouse
别名
- antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD51 antibody
- DKFZp686A08142 antibody
- Integrin alpha five antibody
- integrin alpha V beta 3 antibody
- Integrin alpha-5 antibody
- integrin alpha-V antibody
- Integrin alpha-V light chain antibody
- integrin alphaVbeta3 antibody
- integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
- ITAV_HUMAN antibody
- ITGAV antibody
- MSK 8 antibody
- Msk8 antibody
- Vitronectin receptor subunit alpha antibody
- VNRA antibody
- VTNR antibody
see all

Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/1000 dilution

Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate.
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate treated with PNGase F.

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated.)

Predicted band size: 116 kDa
Observed band size: 116.135 kDa why is the actual band size different from the predicted?

Exposure time: 20 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/1000 dilution

Lane 1 : Untreated K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate.
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) treated with PNGase F whole cell lysate.

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated.)

Predicted band size: 116 kDa
Observed band size: 116.135 kDa why is the actual band size different from the predicted?

Exposure time: 80 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/50000 dilution

Lane 1 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysates
Lane 2 : HT-29 (Human colorectal adenocarcinoma cells) whole cell lysates
Lane 3 : A549 (Human lung carcinoma) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 116 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% NFDM/TBST.

ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution.

Membrane and weak cytoplasm staining on human transitional cell carcinoma of bladder is observed. Counterstained with hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Integrin alpha V was immunoprecipitated from 1 mg of A549 (Human lung carcinoma) whole cell extract with ab179475 at 1/40 dilution. Western blot analysis was performed from the immunoprecipitate using ab179475 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1: A549 whole cell extract.

Lane 2: PBS instead of A549 whole cell extract.

Blocking/Dilution buffer: 5% NFDM/TBST.

ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma) cells labeling Integrin alpha V with ab179475 at 1/500 dilution, followed by Goat anti-rabbit Alexa Fluor^® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green).

Confocal image showing membrane and cytoplasm staining on A549 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor^®594 secondary antibody) at 1/500 dilution (red).

The negative controls are as follows:

-ve control 1: ab179475 at 1/500 dilution followed by ab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/500 dilution.

-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor^®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution

Lane 1 : Human fetal kidney lysates
Lane 2 : Human fetal brain lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 116 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% NFDM/TBST.

ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution.

Membrane and cytoplasm staining on human kidney tubules is observed. Counterstained with hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution

Lane 1 : Mouse brain lysates
Lane 2 : Mouse kidney lysates
Lane 3 : Mouse spleen lysates
Lane 4 : Rat brain lysates
Lane 5 : Rat kidney lysates
Lane 6 : C6 (Rat glial tumor cells) whole cell lysates
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
Lane 8 : Raji (Human Burkitt's lymphoma cell line) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 116 kDa
Observed band size: 125,135 kDa why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% NFDM/TBST.

ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary at 1/500 dilution.

Membrane and cytoplasm staining on mouse kidney tubule and glomerulus is observed. Counterstained with hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)This image is courtesy of an anonymous Abreview.

All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/2000 dilution (8 hours at 4°C)

All lanes : Mouse skin whole cell lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG(H+L)-HRP at 1/5000 dilution

Developed using the ECL technique.

Predicted band size: 116 kDa

Exposure time: 25 seconds

Diluent: 2% BSA in 1X TBST.

Blocked using 5% BSA for 1 hour at RT.

See Abreview
Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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发表研究结果有使用 ab179475？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab179475 被引用在 73 文献中.

Wu JY et al. WDHD1 is over-expressed in nasopharyngeal carcinoma and may control the expression of ITGAV. FEBS Open Bio 13:102-117 (2023). PubMed: 36345604
Szeto ACH et al. An αvβ3 integrin checkpoint is critical for efficient TH2 cell cytokine polarization and potentiation of antigen-specific immunity. Nat Immunol 24:123-135 (2023). PubMed: 36550322
Hayman IR et al. New insight into Epstein-Barr virus infection using models of stratified epithelium. PLoS Pathog 19:e1011040 (2023). PubMed: 36630458
Huang L et al. Inhibition of Integrin αvβ3-FAK-MAPK signaling constrains the invasion of T-ALL cells. Cell Adh Migr 17:1-14 (2023). PubMed: 36944577
Li Mow Chee F et al. Mena regulates nesprin-2 to control actin-nuclear lamina associations, trans-nuclear membrane signalling and gene expression. Nat Commun 14:1602 (2023). PubMed: 36959177

View all Publications for this product

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1-10 of 10 Abreviews or Q&A

Application

Western blot

Sample

Mouse Tissue lysate - whole (heart)

Gel Running Conditions

Reduced Denaturing (4-12)

Loading amount

10 µg

Specification

heart

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 17 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Human prostate tissue)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer (pH 9.0)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

Human prostate tissue

Blocking step

Blocking according to ab210061 - Double stain IHC kit plus goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C

Fixative

No fixation

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

提交于 Aug 11 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Mice prostate tissue)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer (pH 9.0)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

Mice prostate tissue

Blocking step

Blocking according to ab210061 - Double stain IHC kit plus goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C

Fixative

No fixation

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

提交于 Aug 11 2021

Application

Western blot

Sample

Rat Cell lysate - whole cell (Rat hepatocytes)

Gel Running Conditions

Reduced Denaturing (8% SDS-PAGE)

Loading amount

35 µg

Specification

Rat hepatocytes

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

提交于 Aug 11 2021

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (RM-1 - mouse prostate cancer cells)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

RM-1 - mouse prostate cancer cells

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

提交于 Dec 03 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Rat Cell (Rat hepatocytes)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

Rat hepatocytes

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

提交于 Sep 10 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (22Rv1 - prostate cancer cells)

Gel Running Conditions

Reduced Denaturing (8% SDS-PAGE)

Loading amount

25 µg

Specification

22Rv1 - prostate cancer cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

提交于 Aug 31 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (PC-3 - prostate cancer cells)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

PC-3 - prostate cancer cells

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

提交于 Aug 28 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Human skin SCC)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate buffer

Permeabilization

Specification

Human skin SCC

Blocking step

Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Yong Sun Lee

Verified customer

提交于 Jun 27 2018

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Mouse skin)

Gel Running Conditions

Reduced Denaturing (8%)

Loading amount

40 µg

Specification

Mouse skin

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 20 2018

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

应用

The Abpromise guarantee

靶标

功能

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

文献 (73)

客户评价及客户问答

Western blot abreview for Anti-Integrin alpha V antibody [EPR16800]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha V antibody [EPR16800]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha V antibody [EPR16800]

Western blot abreview for Anti-Integrin alpha V antibody [EPR16800]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin alpha V antibody [EPR16800]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin alpha V antibody [EPR16800]

Western blot abreview for Anti-Integrin alpha V antibody [EPR16800]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin alpha V antibody [EPR16800]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha V antibody [EPR16800]

Western blot abreview for Anti-Integrin alpha V antibody [EPR16800]