Anti-Integrin alpha V + beta 6抗体[10D5] - BSA and Azide free (ab77906)
Key features and details
- Mouse monoclonal [10D5] to Integrin alpha V + beta 6 - BSA and Azide free
- Suitable for: Flow Cyt, IP, Blocking
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
概述
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产品名称
Anti-Integrin alpha V + beta 6抗体[10D5] - BSA and Azide free
参阅全部 Integrin alpha V + beta 6 一抗 -
描述
小鼠单克隆抗体[10D5] to Integrin alpha V + beta 6 - BSA and Azide free -
宿主
Mouse -
特异性
ab77906 recognises alpha V beta 6 and does not recognize alpha V or other alpha V integrins. -
经测试应用
适用于: Flow Cyt, IP, Blockingmore details
不适用于: IHC-P or WB -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Tissue, cells or virus corresponding to Mouse Integrin alpha V + beta 6. Wild type murine keratinocytes.
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阳性对照
- H441 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Sterile filtered -
克隆
单克隆 -
克隆编号
10D5 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77906于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
Flow cytometry should be done with buffers containing 1 mM Mg, since the epitope recognized by this antibody is divalent cation dependent. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
| IP |
Use at an assay dependent concentration.
because this antibody is an IgG2a, protein G or rabbit anti mouse IgG is helpful for capture. |
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| Blocking |
Use at an assay dependent concentration.
Inhibition of alpha V beta 6 mediated cell adhesion and cell migration are achieved at a concentration of 100 µg/mL using beta 6 transfected cells tested against TGF beta LAP. |
| 说明 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. Flow cytometry should be done with buffers containing 1 mM Mg, since the epitope recognized by this antibody is divalent cation dependent. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
IP
Use at an assay dependent concentration. because this antibody is an IgG2a, protein G or rabbit anti mouse IgG is helpful for capture. |
|
Blocking
Use at an assay dependent concentration. Inhibition of alpha V beta 6 mediated cell adhesion and cell migration are achieved at a concentration of 100 µg/mL using beta 6 transfected cells tested against TGF beta LAP. |
靶标
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功能
The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3685 Human
- Entrez Gene: 3694 Human
- Entrez Gene: 16410 Mouse
- Entrez Gene: 16420 Mouse
- Entrez Gene: 296456 Rat
- Entrez Gene: 311061 Rat
- Omim: 147558 Human
- Omim: 193210 Human
see all -
别名
- CD51 antibody
- Integrin alpha-V light chain antibody
- integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
see all
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (19)
ab77906 被引用在 19 文献中.
- Sarry M et al. Development of a primary cell model derived from porcine dorsal soft palate for foot-and-mouth disease virus research and diagnosis. Front Microbiol 14:1215347 (2023). PubMed: 37840704
- Wang X et al. Inhibition of Integrin αvβ6 Activation of TGF-β Attenuates Tendinopathy. Adv Sci (Weinh) 9:e2104469 (2022). PubMed: 35166070
- Harvey Y et al. An Improved αvβ6-Receptor-Expressing Suspension Cell Line for Foot-and-Mouth Disease Vaccine Production. Viruses 14:N/A (2022). PubMed: 35337028
- Gringhuis SI et al. Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs. Nat Immunol 23:1735-1748 (2022). PubMed: 36456734
- Kuwada T et al. Identification of an Anti-Integrin avß6 Autoantibody in Patients With Ulcerative Colitis. Gastroenterology 160:2383-2394.e21 (2021). PubMed: 33582126
