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Anti-Integrin alpha 6抗体[MP 4F10] (ab20142)

Reviews (8)Q&A (3)References (23)
Flow Cytometry - Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)
  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)

Key features and details

  • Mouse monoclonal [MP 4F10] to Integrin alpha 6
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2b

概述

  • 产品名称

    Anti-Integrin alpha 6抗体[MP 4F10]
    参阅全部 Integrin alpha 6 一抗

  • 描述

    小鼠单克隆抗体[MP 4F10] to Integrin alpha 6

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt, ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Tissue, cells or virus corresponding to Integrin alpha 6. SW1222 human colorectal cell line.

  • 阳性对照

    • Human lymph node tissue.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab20142于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use 1µg for 106 cells.

(paraformaldehyde or methanol fixed cells)

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF (2)
Use a concentration of 1 µg/ml.
说明
Flow Cyt
Use 1µg for 106 cells.

(paraformaldehyde or methanol fixed cells)

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF
Use a concentration of 1 µg/ml.

靶标

  • 功能

    Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).

  • 组织特异性

    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).

  • 疾病相关

    Epidermolysis bullosa letalis, with pyloric atresia

  • 序列相似性

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.

  • 翻译后修饰

    Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
    In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
    Palmitoylation by DHHC3 enhances stability and cell surface expression.

  • 细胞定位

    Cell membrane. Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Alpha6p antibody
      • CD49 antigen-like family member F antibody
      • CD49f antibody
      • Integrin alpha6B antibody
      • Integrin, alpha 6 antibody
      • ITA6_HUMAN antibody
      • ITGA6 antibody
      • ITGA6B antibody
      • Processed integrin alpha-6 antibody
      • VLA 6 antibody
      • VLA-6 antibody
      see all

    图片

    • Flow Cytometry - Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)
      Flow Cytometry - Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)

      Overlay histogram showing HT1080 cells stained with 6 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HT1080 cells fixed with methanol (5 min) used under the same conditions.

      Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)
      Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)

      ICC/IF image of ab150035). The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M (ab7481) glycine (ab120050) in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab150113) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    文献 (23)

    发表研究结果有使用 ab20142？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab20142 被引用在 23 文献中.

    • Zhou J  et al. Integrin α3/α6 and αV are implicated in ADAM15-activated FAK and EGFR signalling pathway individually and promote non-small-cell lung cancer progression. Cell Death Dis 13:486 (2022). PubMed: 35597804
    • Yang G  et al. Screening of molecular markers of induced chemotherapy in supraglottic laryngeal squamouscell carcinoma. World J Otorhinolaryngol Head Neck Surg 6:34-40 (2020). PubMed: 32426701
    • Jiang K  et al. Exosome-derived ENO1 regulates integrin a6ß4 expression and promotes hepatocellular carcinoma growth and metastasis. Cell Death Dis 11:972 (2020). PubMed: 33184263
    • Leng L  et al. Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth. Stem Cell Res Ther 11:415 (2020). PubMed: 32967725
    • Nattmann A  et al. Analysis of ADAM9 regulation and function in vestibular schwannoma primary cells. BMC Res Notes 13:528 (2020). PubMed: 33176868
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 11 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha 6 antibody [MP 4F10]

     Below Average
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6 10, 20 or 30min
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 15 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha 6 [MP 4F10] antibody

     Inconclusive
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    10% goat serum diluted in 2%BSA/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Antigen retrieval step
    Other
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 03 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin alpha 6 [MP 4F10] antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
    Sample
    Human Cell (HUVEC)
    Specification
    HUVEC
    Permeabilization
    Yes - 0.1% Triton
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 02 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin alpha 6 antibody [MP 4F10]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HUVEC)
    Specification
    HUVEC
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 1°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 17 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha 6 antibody [MP 4F10]

     Inconclusive
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Specification
    Liver
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None - Buffer/Enzyme Used: Citrate 0,01M pH6
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 19 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-Integrin alpha 6 antibody [MP 4F10]

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.2% Trititon X-100
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. XXXXXX XXXXXX

    Verified customer

    提交于 Jul 18 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Integrin alpha 6 antibody [MP 4F10]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Casein as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Vaughan Feisst

    Verified customer

    提交于 Jul 08 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha 6 antibody [MP 4F10]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lymph node)
    Specification
    Lymph node
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 31 2011

    Question

    I am using the antibody ab20142 in human tissue sections,but it seems does not work. SoI am trying to find some pictures other people did with this antibody. In the data sheet, it shows in these papaers PubMed: 17370299, PubMed: 10679006and PubMed: 7674085theauthorsused this product. But unfortunately, I can not find anyfigure that showing theIHC result, and some of them just usedthis antibodyfor FACS many years ago. So, could youhelp me to find aIHC staining inrecent literatures? If you show that one on your web site, please let me knowwhich paper it comes from.

    Read More
    Answer

    Thank you for contacting Abcam.

    I am sorry that you are having some issues with ab20142well, the only image we have of it being used in IHC-P is from an Abreview and that information can be found below:

    https://www.abcam.com/Integrin-alpha-6-antibody-MP-4F10-ab20142.html&tab=abreviews&intabreviewid=24332

    The antibody is covered under our Abpromise for six months and is guaranteed to work in IHC-P on human samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund. To be able to better help in this matter, could you please send me a copy of the protocol that you are using, to see if there is anything I can suggest that will help.

    Looking forward to your reply and helping resolve this issue.

    Read More

    Question

    Dear technical team, Our customer would like to purchase 11 antibodies from abcam and needs control for each of antibody. The customer will test IHC-P so, control should be slide type. I have searched only one of them as below and would like to ask check the rest of them.  

    Read More
    Answer

    Thank you very much for contacting us - and sending the enquiry as a table. That clear overview was very helpful :-) As you may know, we have not tested the individual samples for their expression of the mentioned targets. However, according to the literature and other sources such as http://www.proteinatlas.org/ these tissues exhibit high expression of these proteins and thus are suitable controls. Cat# Product Positive control Slide ab27526 Anti-Notch1 antibody Breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696) ab7970 Anti-NFkB p65 antibody Spleen (or skin) ab4372 (or ab4365, ab4417)　 ab10800 Anti-Integrin alpha 2 antibody Bladder, gall bladder, kidney　 ab4287, ab4330, ab4347　 ab20142 Anti-Integrin alpha 6 antibody Bladder, gall bladder, oesophagus　 ab4287, ab4330, ab4404　 ab16821 Anti-Integrin alpha V antibody Human breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696)　 ab52971 Anti-Integrin beta 1 antibody Human breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696)　 ab75872 Anti-Integrin beta 3 antibody Human spleen lysate and human tonsil tissue. ab4372, ab5146 ab79007 Anti-Integrin alpha 1 + beta 1 antibody Rat gut, heart and lung tissue. ab4620, ab4617　 ab30483 Anti-Integrin alpha 2+beta 1 antibody Bladder, gall bladder,breast carcinoma ab4287, ab4330, ab4697, ab4698 (but also ab4701, ab4700, ab4696)　 ab16822 Anti-Integrin alpha 2b + beta 3 antibody Spleen, kidney　 ab4372, ab4347　 ab78289 Anti-Integrin alpha V beta 3 antibody Bladder, oesophagus　 ab4287, ab4404　 All the above mentioned antibodies are guaranteed to work in immunohistochemistry on paraffin-embedded sections as stated on the datasheet. However, we do not currently have an image of ab10800, ab16821, ab30483, ab16822, and ab78289 in IHC-P. Therefore, I can offer a discount off a future purchase if you buy one of these antibodies now, use it IHC-P and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody each. If you decide to go ahead and purchase this product, please contact our Scientific Support team prior to the purchase for more details. I hope this information is helpful to you. Please do not hesitate to contact me if you need any further advice or information in this regard.

    Read More

    1-10 of 11 Abreviews or Q&A

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