Anti-Integrin alpha 6抗体[GoH3] (ab105669)
![Flow Cytometry - Anti-Integrin alpha 6 antibody [GoH3] (ab105669)](https://www.abcam.cn/ps/products/105/ab105669/Images/ab105669-118071-anti-integrin-alpha-6-antibody-goh3-flow-cytometry.gif "Flow Cytometry - Anti-Integrin alpha 6 antibody [GoH3] (ab105669)")
Key features and details
- Rat monoclonal [GoH3] to Integrin alpha 6
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
Anti-Integrin alpha 6抗体[GoH3]
参阅全部 Integrin alpha 6 一抗 -
描述
大鼠单克隆抗体[GoH3] to Integrin alpha 6 -
宿主
Rat -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Integrin alpha 6. Mouse mammary tumor cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
单克隆 -
克隆编号
GoH3 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab105669于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
| 说明 |
|---|
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Flow Cyt
Use at an assay dependent concentration. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
靶标
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功能
Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). -
组织特异性
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). -
疾病相关
Epidermolysis bullosa letalis, with pyloric atresia -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
翻译后修饰
Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
Palmitoylation by DHHC3 enhances stability and cell surface expression. -
细胞定位
Cell membrane. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3655 Human
- Omim: 147556 Human
- SwissProt: P23229 Human
- Unigene: 133397 Human
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别名
- Alpha6p antibody
- CD49 antigen-like family member F antibody
- CD49f antibody
see all
图片
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Flow Cytometry - Anti-Integrin alpha 6 antibody [GoH3] (ab105669)Staining of normal human peripheral blood cells with 0.25 ug of Rat IgG2a Isotype Control Purified (open histogram)or 0.25 ug of Anti-Human/Mouse CD49f (Integrin alpha 6) Purified (filled histogram) followed by Anti-Rat IgG PE Cells in the lymphocyte gate were used for analysis
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab105669 被引用在 16 文献中.
- Franken A et al. Comparative analysis of EpCAM high-expressing and low-expressing circulating tumour cells with regard to their clonal relationship and clinical value. Br J Cancer 128:1742-1752 (2023). PubMed: 36823365
- Kechagia Z et al. The laminin-keratin link shields the nucleus from mechanical deformation and signalling. Nat Mater 22:1409-1420 (2023). PubMed: 37709930
- Kondo K et al. Identification of distinct N-glycosylation patterns on extracellular vesicles from small-cell and non-small-cell lung cancer cells. J Biol Chem 298:101950 (2022). PubMed: 35447118
- Guo D et al. Ontogenesis of the tear drainage system requires Prickle1-driven polarized basement membrane deposition. Development 147:N/A (2020). PubMed: 33144400
- Zhang K & Manninen A 3D Cell Culture Models of Epithelial Tissues. Methods Mol Biol 1926:77-84 (2019). PubMed: 30742264