重组Anti-Integrin alpha 6抗体[EPR5578] (ab124924)
![Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)](https://www.abcam.cn/ps/products/124/ab124924/Images/ab124924-449294-anti-integrin-alpha-6-antibody-epr5578-western-blot-sw480-wt-sw480-itga6-knockout-hek293.jpg "Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5578] to Integrin alpha 6
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Integrin alpha 6抗体[EPR5578]
参阅全部 Integrin alpha 6 一抗 -
描述
兔单克隆抗体[EPR5578] to Integrin alpha 6 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Integrin alpha 6 aa 100-200. The exact sequence is proprietary.
Database link: P23229 -
阳性对照
- SW480, HEK293, JAR, HT1376, and Human platelet lysates; Human kidney tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 2.29 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5578 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124924于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).
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| 说明 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa). |
靶标
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功能
Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). -
组织特异性
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). -
疾病相关
Epidermolysis bullosa letalis, with pyloric atresia -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
翻译后修饰
Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
Palmitoylation by DHHC3 enhances stability and cell surface expression. -
细胞定位
Cell membrane. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3655 Human
- Omim: 147556 Human
- SwissProt: P23229 Human
- Unigene: 133397 Human
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别名
- Alpha6p antibody
- CD49 antigen-like family member F antibody
- CD49f antibody
see all
图片
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Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)All lanes : Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) at 1/1000 dilution
Lane 1 : Wild-type SW480 cell lysate
Lane 2 : ITGA6 knockout SW480 cell lysate
Lane 3 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 126 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab124924 observed at 130 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab124924 was shown to react with Integrin alpha 6 in wild-type SW480 cells in western blot with loss of signal observed in ITGA6 knockout cell line ab265838 (knockout cell lysate ab256957). Wild-type and ITGA6 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab124924 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)](https://www.abcam.cn/ps/products/124/ab124924/Images/ab124924-254377-anti-integrin-alpha-6-antibody-epr5578-western-blot.jpg)
Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)All lanes : Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) at 1/10000 dilution (purified)
Lane 1 : HT-1376 cell lysate
Lane 2 : JAR cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 126 kDa
Observed band size: 126 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)](https://www.abcam.cn/ps/products/124/ab124924/Images/ab124924-138938-anti-integrin-alpha-6-antibody-epr5578-western-blot.jpg)
Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)All lanes : Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) at 1/1000 dilution
Lane 1 : JAR cell lysate
Lane 2 : HT1376 cell lysate
Lane 3 : Human platelet lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 126 kDaSecondary antibody - anti-rabbit HRP (ab6721)
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![OI-RD Scanning - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)](https://www.abcam.cn/ps/products/124/ab124924/Images/ab124924-138936-anti-integrin-alpha-6-antibody-epr5578.jpg)
OI-RD Scanning - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) -
![Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)](https://www.abcam.cn/ps/products/124/ab124924/Images/ab124924-5-benefits-of-recombinant-antibodies.png)
Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab124924 被引用在 5 文献中.
- Kurita T et al. Pathological mechanisms of laryngeal papillomatosis based on laryngeal epithelial characteristics. Laryngoscope Investig Otolaryngol 4:89-94 (2019). PubMed: 30828624
- Hu T et al. Integrin a6/Akt/Erk signaling is essential for human breast cancer resistance to radiotherapy. Sci Rep 6:33376 (2016). PubMed: 27624978
- Ocampo A et al. In Vivo Amelioration of Age-Associated Hallmarks by Partial Reprogramming. Cell 167:1719-1733.e12 (2016). PubMed: 27984723
- Suprynowicz FA et al. Conditionally reprogrammed cells represent a stem-like state of adult epithelial cells. Proc Natl Acad Sci U S A 109:20035-40 (2012). WB ; Human . PubMed: 23169653
- Kariya Y & Gu J N-glycosylation of ß4 integrin controls the adhesion and motility of keratinocytes. PLoS One 6:e27084 (2011). WB . PubMed: 22073258