Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-Integrin alpha 4/CD49D (phospho S988)抗体[EPR2343] (ab75760)

Reviews (1) Submit a question References (1)
Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)
  • Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)
  • Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)
  • Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2343] to Integrin alpha 4/CD49D (phospho S988)
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Integrin alpha 4/CD49D (phospho S988)抗体[EPR2343]
    参阅全部 Integrin alpha 4/CD49D 一抗

  • 描述

    兔单克隆抗体[EPR2343] to Integrin alpha 4/CD49D (phospho S988)

  • 宿主

    Rabbit

  • 特异性

    ab75760 only detects Integrin alpha 4/CD49D phosphorylated on Serine 988 of the mature protein. S988 coresponds to S1021 of the sequence with the 33 aa signal peptide and S1027 of the precursor shown in Figure 4 of PMID: 8436405.

    The antibody detects the mature chain (180 kDa), the precursor (140 kDa) and the cleaved C terminal (70 kDa).

     

  • 经测试应用

    适用于: WBmore details
    不适用于: ICC/IF or IHC-P

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • THP1 cell lysates

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab75760于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/500 - 1/2000. Predicted molecular weight: 150 kDa.
说明
WB
1/500 - 1/2000. Predicted molecular weight: 150 kDa.
应用说明
Is unsuitable for ICC/IF or IHC-P.

靶标

  • 功能

    Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.

  • 序列相似性

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.

  • 结构域

    The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.

  • 翻译后修饰

    Phosphorylation on Ser-1027 inhibits PXN binding.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 269C wild type antibody
      • Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
      • CD49 antigen like family member D antibody
      • CD49 antigen-like family member D antibody
      • CD49d antibody
      • IA4 antibody
      • Integrin alpha 4 antibody
      • Integrin alpha 4 subunit antibody
      • Integrin alpha IV antibody
      • Integrin alpha-4 antibody
      • Integrin alpha-IV antibody
      • Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor) antibody
      • ITA4_HUMAN antibody
      • ITGA4 antibody
      • MGC90518 antibody
      • OTTHUMP00000205320 antibody
      • Very late activation protein 4 receptor, alpha 4 subunit antibody
      • VLA 4 subunit alpha antibody
      • VLA-4 subunit alpha antibody
      • VLA4 antibody
      see all

    图片

    • Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)
      Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)
      All lanes : Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760) at 1/1000 dilution (purified)

      Lane 1 : Rat spleen tissue lysate
      Lane 2 : Mouse spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 150 kDa
      Observed band size: 150,70 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)
      Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)
      All lanes : Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760) at 1/5000 dilution (purified)

      Lane 1 : untreated Jurkat lysate
      Lane 2 : Jurkat cell lysate treated with Lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size: 150 kDa
      Observed band size: 140,150,70 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)
      Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)
      All lanes : Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760) at 1/2000 dilution

      Lane 1 : THP1 cell lysates, untreated
      Lane 2 : THP1 cell lysates, with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 150 kDa
      Observed band size: 140,180,70 kDa why is the actual band size different from the predicted?

    • Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)
      Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)

    文献 (1)

    发表研究结果有使用 ab75760？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab75760 被引用在 1 文献中.

    • Lin ZH  et al. The effects of estradiol on inflammatory and endothelial dysfunction in rats with preeclampsia. Int J Mol Med 45:825-835 (2020). PubMed: 31985028

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Western blot abreview for Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEF)
    Gel Running Conditions
    Non-reduced Denaturing (8%)
    Loading amount
    20 µg
    Specification
    MEF
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 01 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com