Anti-Integrin alpha 4/CD49D抗体[HP2/1] (ab22858)
![Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 4/CD49D antibody [HP2/1] (ab22858)](https://www.abcam.cn/ps/products/22/ab22858/Images/ab22858-287538-anti-integrin-alpha-4-antibody-hp2-1-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 4/CD49D antibody [HP2/1] (ab22858)")
Key features and details
- Mouse monoclonal [HP2/1] to Integrin alpha 4/CD49D
- Suitable for: ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Integrin alpha 4/CD49D抗体[HP2/1]
参阅全部 Integrin alpha 4/CD49D 一抗 -
描述
小鼠单克隆抗体[HP2/1] to Integrin alpha 4/CD49D -
宿主
Mouse -
特异性
This antibody recognises the 80kD alpha chain of VLA-4. Reacts with monocytes, T cells, B cells, thymocytes and Langerhans cells. -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human Integrin alpha 4/CD49D. JM leukaemia line.
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阳性对照
- ICC/IF: wildtype HAP-1 cells.
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常规说明
Can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure. HP2/1 inhibits cell binding to soluble VCAM-1.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
Primary antibody说明
Can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure. HP2/1 inhibits cell binding to soluble VCAM-1. -
克隆
单克隆 -
克隆编号
HP2/1 -
骨髓瘤
x63-Ag8.653 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab22858于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
结构域
The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion. -
翻译后修饰
Phosphorylation on Ser-1027 inhibits PXN binding. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3676 Human
- Omim: 192975 Human
- SwissProt: P13612 Human
- Unigene: 440955 Human
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别名
- 269C wild type antibody
- Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
- CD49 antigen like family member D antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 4/CD49D antibody [HP2/1] (ab22858)
ab22858 staining Integrin alpha 4/CD49D in wild-type HAP1 cells (top panel) and Integrin alpha 4/CD49D knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab22858 at 5μg/ml concentration and ab202272 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab22858 被引用在 6 文献中.
- Connolly NP et al. Elevated fibroblast growth factor-inducible 14 expression transforms proneural-like gliomas into more aggressive and lethal brain cancer. Glia 69:2199-2214 (2021). PubMed: 33991013
- Langel SN et al. Stage of Gestation at Porcine Epidemic Diarrhea Virus Infection of Pregnant Swine Impacts Maternal Immunity and Lactogenic Immune Protection of Neonatal Suckling Piglets. Front Immunol 10:727 (2019). PubMed: 31068924
- Langel SN et al. Oral vitamin A supplementation of porcine epidemic diarrhea virus infected gilts enhances IgA and lactogenic immune protection of nursing piglets. Vet Res 50:101 (2019). PubMed: 31783923
- Zhang YL et al. SPON2 Promotes M1-like Macrophage Recruitment and Inhibits Hepatocellular Carcinoma Metastasis by Distinct Integrin-Rho GTPase-Hippo Pathways. Cancer Res 78:2305-2317 (2018). PubMed: 29440144
- Connolly NP et al. Genetically engineered rat gliomas: PDGF-driven tumor initiation and progression in tv-a transgenic rats recreate key features of human brain cancer. PLoS One 12:e0174557 (2017). IHC-Fr ; Rat . PubMed: 28358926
- Qi J et al. Identification, characterization, and epitope mapping of human monoclonal antibody J19 that specifically recognizes activated integrin a4ß7. J Biol Chem 287:15749-59 (2012). Flow Cyt ; Human . PubMed: 22418441