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AB275979

重组Anti-Integrin alpha 4/CD49D抗体[Natalizumab] -兔IgG (Chimeric)

Anti-Integrin alpha 4/CD49D antibody [Natalizumab] - Rabbit IgG (Chimeric)

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Rabbit Recombinant Monoclonal Integrin alpha 4/CD49D antibody. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing ITGA4 protein.

查看别名

CD49d, CD49D, ITGA4, Integrin alpha-4, CD49 antigen-like family member D, Integrin alpha-IV, VLA-4 subunit alpha

1 Images
Flow Cytometry - Anti-Integrin alpha 4/CD49D antibody [Natalizumab] - Rabbit IgG (Chimeric) (AB275979)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Integrin alpha 4/CD49D antibody [Natalizumab] - Rabbit IgG (Chimeric) (AB275979)

Flow cytometry of human lymphocytes labeling Integrin alpha 4/CD49D with ab275979 (panel B) compared with an isotype control (panel A) at 1 μg/ml for 30 mins at RT. After washing, bound antibody was detected using Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)) and cells analysed on a FACSCanto flow-cytometer.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

Natalizumab

亚型

IgG

轻链类型

kappa

不含载体蛋白

No

反应种属

Human

应用

Flow Cyt

applications

免疫原

Cell preparation containing ITGA4 protein. The exact immunogen used to generate this antibody is proprietary information.

P13612

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }
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产品详情

This rabbit monoclonal chimeric antibody was made using the variable domain sequences of the original human IgG4 format, for improved compatibility with existing reagents, assays and techniques.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Preservative: 0.02% Proclin 300 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 4 also known as CD49D or ITGA4 is a transmembrane receptor with a mass of approximately 150 kDa. This protein is expressed on the surface of leukocytes including T and B lymphocytes monocytes and eosinophils. Integrin alpha 4 functions by interacting mechanically with the extracellular matrix components and endothelial cell counter-receptors like VCAM-1 facilitating cell adhesion and migration. Its expression is vital for the immune cells' ability to move and locate sites of inflammation or injury.
Biological function summary

Integrin alpha 4 forms a heterodimer complex with beta subunits like beta 1 (forming VLA-4) or beta 7 allowing it to mediate adhesion and signaling pathways. In particular the alpha 4 and beta 1 dimerization gives rise to a high-affinity receptor for fibronectin and VCAM-1. This interaction is essential for regulating immune responses and maintaining cell positioning within tissues. The presence of this complex on leukocytes drives their ability to navigate through the vasculature to inflamed areas.

Pathways

Integrin alpha 4 participates in significant signal transduction pathways including the MAPK and PI3K-Akt pathways. These pathways modulate cell survival proliferation and migration. In the MAPK pathway integrin alpha 4 influences cell signaling cascades by interacting with other proteins like TALIN or PAXILLIN which are important for integrin-mediated signal relay. In connection with PI3K-Akt pathway alpha 4 integrins work closely with proteins in regulating cellular adhesion and motility showing a dynamic interplay in immune regulation.

Integrin alpha 4 plays an important role in various immune-related diseases such as multiple sclerosis and Crohn's disease. The protein's participation in leukocyte adhesion and trafficking makes it a target for therapeutic intervention. Natalizumab a monoclonal antibody against alpha 4 integrin showcases the critical link between the protein and disease treatment by blocking leukocyte migration to reduce inflammation. Additionally integrin alpha 4's interaction with proteins like VCAM-1 and VLA-4 highlights its relevance in these autoimmune conditions highlighting its importance in disease pathophysiology.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4 : ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415). ITGA4 : ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). Integrin ITGA4 : ITGB1 represses PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via its interaction with SVEP1, thereby inhibiting vasocontraction (PubMed : 35802072).
See full target information ITGA4
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搭配使用产品

Primary Antibodies

AB30394

Anti-Integrin beta 1 antibody [12G10]

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 1 antibody [12G10] (AB30394)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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  • 5
  • 20
Primary Antibodies

AB68139

Anti-LAT (phospho Y200) antibody [EP983(2)Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-LAT (phospho Y200) antibody [EP983(2)Y] (AB68139)

  • 0
  • 0
Primary Antibodies

AB13219

Anti-CD11+CD18 antibody [24]

  • 4
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