Anti-Integrin alpha 3抗体(ab223661)
Key features and details
- Goat polyclonal to Integrin alpha 3
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Integrin alpha 3抗体
参阅全部 Integrin alpha 3 一抗 -
描述
山羊多克隆抗体to Integrin alpha 3 -
宿主
Goat -
特异性
This antibody is expected to recognize reported isoform a (NP_002195.1). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow, Chinese hamster
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免疫原
Synthetic peptide corresponding to Human Integrin alpha 3 aa 1000 to the C-terminus (C terminal) (Cysteine residue).
Database link: P26006 -
阳性对照
- WB: Human heart lysate; mouse heart lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223661于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 117 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 117 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. -
组织特异性
Isoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
翻译后修饰
Isoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12-myristate 13-acetate stimulation. Isoform alpha-3A is phosphorylated on Tyr-1051. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 100758471 Chinese hamster
- Entrez Gene: 508490 Cow
- Entrez Gene: 3675 Human
- Entrez Gene: 16400 Mouse
- Omim: 605025 Human
- SwissProt: P17852 Chinese hamster
- SwissProt: F1MMS9 Cow
- SwissProt: P26006 Human
see all -
别名
- AA407068 antibody
- Antigen identified by monoclonal antibody J143 antibody
- CD49 antigen like family member C antibody
see all
图片
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Western blot - Anti-Integrin alpha 3 antibody (ab223661)Lane 1 : Anti-Integrin alpha 3 antibody (ab223661) at 1 µg/ml
Lane 2 : Anti-Integrin alpha 3 antibody (ab223661) at 1 mg/ml
Lane 1 : Human heart lysate
Lane 2 : Mouse heart lysate
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 117 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab223661 尚未被引用在任何文献中。
