Anti-Integrin alpha 2抗体[HAS-3] (ab10800)
Key features and details
- Mouse monoclonal [HAS-3] to Integrin alpha 2
- Suitable for: IHC-P, IHC-Fr, Flow Cyt, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-Integrin alpha 2抗体[HAS-3]
参阅全部 Integrin alpha 2 一抗 -
描述
小鼠单克隆抗体[HAS-3] to Integrin alpha 2 -
宿主
Mouse -
经测试应用
适用于: IHC-P, IHC-Fr, Flow Cyt, IP, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human Integrin alpha 2. Whole human keratinocytes.
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常规说明
The antibody co-precipitates alpha 2 beta 1 integrin from keratinocytes and inhibits HGF induced branching morphogenesis and motility of human mammary epithelial HB2 cells in collagen.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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纯度
Protein A purified -
Primary antibody说明
The antibody co-precipitates alpha 2 beta 1 integrin from keratinocytes and inhibits HGF induced branching morphogenesis and motility of human mammary epithelial HB2 cells in collagen. -
克隆
单克隆 -
克隆编号
HAS-3 -
骨髓瘤
Sp2 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10800于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
|
|
ICC/IF | (2) |
Use at an assay dependent concentration.
|
说明 |
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IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
结构域
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3673 Human
- Omim: 192974 Human
- SwissProt: P17301 Human
- Unigene: 482077 Human
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别名
- BR antibody
- CD 49b antibody
- CD49 antigen like family member B antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab10800 被引用在 7 文献中.
- Hughes JH et al. Transcriptomic analysis reveals that BMP4 sensitizes glioblastoma tumor-initiating cells to mechanical cues. Matrix Biol 85-86:112-127 (2020). PubMed: 31189077
- Xing Y et al. Regulation of opticin on bioactivity of retinal vascular endothelial cells cultured in collagen. Int J Ophthalmol 13:1202-1209 (2020). PubMed: 32821673
- van Leenders GJ et al. Activation of c-MET Induces a Stem-Like Phenotype in Human Prostate Cancer. PLoS One 6:e26753 (2011). IHC-Fr ; Human . PubMed: 22110593
- Singh SR et al. Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways? Am J Physiol Lung Cell Mol Physiol 299:L169-83 (2010). ICC/IF ; Human . PubMed: 20435685
- Alford D et al. Integrin-matrix interactions affect the form of the structures developing from human mammary epithelial cells in collagen or fibrin gels. J Cell Sci 111 Pt 4:521-32 (1998). PubMed: 9443900
- Sugiyama M et al. Comparison of integrin expression and terminal differentiation capacity in cell lines derived from oral squamous cell carcinomas. Carcinogenesis 14:2171-6 (1993). PubMed: 7693359
- Tenchini ML et al. Evidence against a major role for integrins in calcium-dependent intercellular adhesion of epidermal keratinocytes. Cell Adhes Commun 1:55-66 (1993). PubMed: 7521749