AB109432

重组Anti-Integrin alpha 2抗体[EPR5789]

Anti-Integrin alpha 2 antibody [EPR5789]

RabMAb
Recombinant
KO Validated
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal Integrin alpha 2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

查看别名

CD49b, CD49B, ITGA2, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, GPIa

3 Images

Lab

Western blot - Anti-Integrin alpha 2 antibody [EPR5789] (AB109432)

Lanes 1 - 4 : Merged signal (red and green). Green - ab109432 observed at 165 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab109432 was shown to specifically react with Integrin alpha 2 when Integrin alpha 2 knockout samples were used. Wild-type and Integrin alpha 2 knockout samples were subjected to SDS-PAGE. ab109432 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Integrin alpha 2 antibody [EPR5789] (ab109432) at 1/1000 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

Integrin alpha 2 knockout HAP1 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

T47D cell lysate at 20 µg

Predicted band size: 129 kDa

false

Lab

Western blot - Anti-Integrin alpha 2 antibody [EPR5789] (AB109432)

Lanes 1-4 : Merged signal (red and green). Green - ab109432 observed at 129 kDa. Red - loading control ab8245 observed at 37 kDa.

ab109432 Anti-Integrin alpha 2 antibody [EPR5789] was shown to specifically react with Integrin alpha 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264819 (knockout cell lysate ab257262) was used. Wild-type and Integrin alpha 2 knockout samples were subjected to SDS-PAGE. ab109432 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Integrin alpha 2 antibody [EPR5789] (ab109432) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ITGA2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ITGA2 (CD49b) knockout HeLa cell line (<a href='/products/cell-lines/human-itga2-cd49b-knockout-hela-cell-line-ab264819'>ab264819</a>)

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Predicted band size: 129 kDa,130 kDa

Observed band size: 129 kDa

false

Unknown

Western blot - Anti-Integrin alpha 2 antibody [EPR5789] (AB109432)

All lanes:

Western blot - Anti-Integrin alpha 2 antibody [EPR5789] (ab109432) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

T47-D cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Predicted band size: 129 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5789

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 2 also known as CD49b ITGA2 and A2 is a protein that functions as part of the integrin family. Integrins are transmembrane receptors that facilitate cell-extracellular matrix adhesion. Integrin alpha 2 has a molecular mass of approximately 130 kDa and is widely expressed in epithelial cells fibroblasts and platelets. This protein is important for mediating adhesion between cells and the extracellular matrix affecting cell signaling and behavior. Through this adhesion cells can communicate with their environment adjusting their functions accordingly.

Biological function summary

Integrin alpha 2 plays a significant role in cellular processes like migration and proliferation. It forms a heterodimer complex by pairing with the beta 1-integrin subunit contributing to its adhesive and signaling functions. This association enables the integrin alpha 2 complex to bind specific extracellular matrix components such as collagen and laminin. Through these interactions it regulates processes like wound healing and tissue remodeling and affects how cells respond to mechanical stress.

Pathways

Integrin alpha 2 is involved in major signaling routes that influence cell fate and movement. Notably it participates in the PI3K/AKT and MAPK pathways which are important for regulating cell growth and survival. It interacts with signaling proteins like focal adhesion kinase (FAK) and Src family kinases linking extracellular matrix changes to intracellular responses. These pathways help in transmitting signals that coordinate the cell's structural and functional adjustments in response to its surroundings.

Integrin alpha 2 has connections to cancer and fibrosis. In cancers its altered expression may contribute to tumor progression and metastasis often involving interactions with other integrins and matrix metalloproteinases that degrade extracellular components. In fibrosis excessive integrin alpha 2 activity can lead to abnormal tissue remodeling and scarring implicating its role alongside collagen-producing cells. Understanding these connections is vital for developing therapies targeting integrin-mediated pathways in these diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human rotavirus A.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human echoviruses 1 and 8.

See full target information ITGA2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 6:e27097 PubMed22073262

2011

Gingival fibroblasts display reduced adhesion and spreading on extracellular matrix: a possible basis for scarless tissue repair?

Applications

Species

Human

Fen Guo,David E Carter,Anuradha Mukhopadhyay,Andrew Leask

View all publications

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详情请参阅我们的条款与条件。

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For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Integrin alpha 2抗体[EPR5789]

Anti-Integrin alpha 2 antibody [EPR5789]

Western blot - Anti-Integrin alpha 2 antibody [EPR5789] (AB109432)

Western blot - Anti-Integrin alpha 2 antibody [EPR5789] (AB109432)

Western blot - Anti-Integrin alpha 2 antibody [EPR5789] (AB109432)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原