重组Anti-Integrin alpha 2抗体[EPR5788] (ab133557)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5788] to Integrin alpha 2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
-
产品名称
Anti-Integrin alpha 2抗体[EPR5788]
参阅全部 Integrin alpha 2 一抗 -
描述
兔单克隆抗体[EPR5788] to Integrin alpha 2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt (Intra) or ICC/IF -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human Integrin alpha 2 aa 1-100 (extracellular). The exact sequence is proprietary.
-
阳性对照
- WB: T47-D, HEK293, 293T and A431 cell lysates. Human placenta and mouse spleen tissue lysates. IHC-P: Human breast carcinoma and colon tissue.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 3.20 x 10 -12 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5788 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
- Anti-Integrin alpha 2 antibody [EPR5788] - Low endotoxin, Azide free (ab221214)
- Anti-Integrin alpha 2 antibody [EPR5788] - BSA and Azide free (ab271894)
- PE Anti-Integrin alpha 2 antibody [EPR5788] (ab317897)
- APC Anti-Integrin alpha 2 antibody [EPR5788] (ab317935)
- Alexa Fluor® 488 Anti-Integrin alpha 2 antibody [EPR5788] (ab317975)
- Alexa Fluor® 594 Anti-Integrin alpha 2 antibody [EPR5788] (ab318016)
- Alexa Fluor® 555 Anti-Integrin alpha 2 antibody [EPR5788] (ab318058)
- Alexa Fluor® 647 Anti-Integrin alpha 2 antibody [EPR5788] (ab318100)
- Alexa Fluor® 750 Anti-Integrin alpha 2 antibody [EPR5788] (ab321053)
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133557于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 129 kDa).
|
|
IHC-P | (3) |
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
说明 |
---|
WB
1/10000 - 1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 129 kDa). |
IHC-P
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
-
功能
Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
结构域
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 3673 Human
- Entrez Gene: 16398 Mouse
- Omim: 192974 Human
- SwissProt: P17301 Human
- SwissProt: Q62469 Mouse
- Unigene: 482077 Human
- Unigene: 5007 Mouse
-
别名
- BR antibody
- CD 49b antibody
- CD49 antigen like family member B antibody
see all
图片
-
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Integrin alpha 2 knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: T47D cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133557 observed at 165 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133557 was shown to specifically react with Integrin alpha 2 when Integrin alpha 2 knockout samples were used. Wild-type and Integrin alpha 2 knockout samples were subjected to SDS-PAGE. ab133557 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging. -
All lanes : Anti-Integrin alpha 2 antibody [EPR5788] (ab133557) at 1/10000 dilution (purified)
Lane 1 : T47-D cell lysate
Lane 2 : HEK293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 129 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
Anti-Integrin alpha 2 antibody [EPR5788] (ab133557) at 1/50000 dilution (purified) + A431 cell lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 129 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
Anti-Integrin alpha 2 antibody [EPR5788] (ab133557) at 1/10000 dilution (purified) + Mouse spleen tissue lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 129 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
All lanes : Anti-Integrin alpha 2 antibody [EPR5788] (ab133557) at 1/10000 dilution (unpurified)
Lane 1 : T47D lysate
Lane 2 : 293T lysate
Lane 3 : Human platelet lysate
Lane 4 : A431 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 129 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR5788] (ab133557)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Integrin alpha 2 with purified ab133557 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR5788] (ab133557)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Integrin alpha 2 with unpurified ab133557 at 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
-
SDS download
-
Datasheet download
文献 (54)
ab133557 被引用在 54 文献中.
- Li X et al. Erianin Controls Collagen-Mediated Retinal Angiogenesis via the RhoA/ROCK1 Signaling Pathway Induced by the alpha2/beta1 Integrin-Collagen Interaction. Invest Ophthalmol Vis Sci 63:27 (2022). PubMed: 35060996
- Hoare JI et al. Carvedilol targets β-arrestins to rewire innate immunity and improve oncolytic adenoviral therapy. Commun Biol 5:106 (2022). PubMed: 35115660
- Wu C et al. Gene Coexpression Network Characterizing Microenvironmental Heterogeneity and Intercellular Communication in Pancreatic Ductal Adenocarcinoma: Implications of Prognostic Significance and Therapeutic Target. Front Oncol 12:840474 (2022). PubMed: 35719923
- Wang Y et al. Maslinic Acid Inhibits the Growth of Malignant Gliomas by Inducing Apoptosis via MAPK Signaling. J Oncol 2022:3347235 (2022). PubMed: 35799612
- Chen WJ et al. Podocalyxin-Like Protein 1 Regulates Pluripotency through the Cholesterol Biosynthesis Pathway. Adv Sci (Weinh) 10:e2205451 (2022). PubMed: 36373710