AB109538

重组Anti-Insulin degrading enzyme / IDE抗体[EPR6099]

Anti-Insulin degrading enzyme / IDE antibody [EPR6099]

RabMAb
Recombinant
KO Validated
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(4 Publications)

Rabbit Recombinant Monoclonal Insulin degrading enzyme / IDE antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

查看别名

Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease, Insulysin, Insulinase, IDE

4 Images

Flow Cytometry (Intracellular) - Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (AB109538)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (AB109538)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Insulin degrading enzyme / IDE with purified ab109538 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluorr^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Lab

Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (AB109538)

Lanes 1 - 4 : Merged signal (red and green). Green - ab109538 observed at 118 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab109538 was shown to specifically react with IDE in wild-type HAP1 cells. No band was observed when IDE knockout samples were examined. Wild-type and IDE knockout samples were subjected to SDS-PAGE. ab109538 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab109538) at 1/2000 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

IDE knockout HAP1 cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Predicted band size: 118 kDa

false

Lab

Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (AB109538)

Lanes 1- 2 : Merged signal (red and green). Green - ab109538 observed at 118 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab109538 was shown to react with Insulin degrading enzyme / IDE in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261755 (knockout cell lysate ab257197) was used. Wild-type HeLa and IDE knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109538 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab109538) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

IDE knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human IDE (Insulin degrading enzyme) knockout HeLa cell line (<a href='/products/cell-lines/human-ide-insulin-degrading-enzyme-knockout-hela-cell-line-ab261755'>ab261755</a>)

Predicted band size: 118 kDa

Observed band size: 118 kDa

false

Unknown

Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (AB109538)

All lanes:

Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab109538) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

A375 cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 118 kDa

false

不同偶联物与剂型 (2)

HRP

HRP Anti-Insulin degrading enzyme / IDE antibody [EPR6099]
Carrier free

Anti-Insulin degrading enzyme / IDE antibody [EPR6099] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR6099

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Insulin degrading enzyme (IDE) also known as insulinase is a zinc metalloprotease involved in the breakdown of small proteins including insulin. IDE has a molecular weight of approximately 110 kDa. It works by cleaving the peptide bonds of its substrate proteins therefore decreasing their molecular integrity. IDE is expressed in several tissues including the liver muscle and kidney where it plays a significant role in regulating metabolic processes. This protein can be found both within cells and in the extracellular space.

Biological function summary

IDE manages the levels of insulin and other peptides by degrading them preventing accumulation and maintaining homeostasis. It is not part of a complex but it acts individually in cellular environments to modulate the concentration of its substrates. IDE is important for controlling insulin availability and turnover which impacts glucose metabolism. By influencing the degradation of insulin IDE aids in balancing metabolic demands with insulin availability.

Pathways

IDE plays a vital role in insulin signaling and glucose metabolic processes. It is directly involved in the insulin signaling pathway by regulating insulin levels which consequently affects cellular responses to insulin. IDE connects with several proteins associated with these pathways including insulin receptor and glucose transporters ensuring proper cell signaling and metabolic functions. By modulating insulin levels IDE helps optimize glucose uptake and storage.

IDE has a relevant connection to Alzheimer's disease and type 2 diabetes. Its role in insulin degradation links it to type 2 diabetes where dysregulation of insulin levels can exacerbate the disease. IDE is also associated with Alzheimer's disease since it degrades amyloid-beta peptides. Any malfunction or altered expression of IDE can lead to accumulation of these peptides contributing to Alzheimer's pathology. In the context of these diseases IDE interacts with amyloid-beta precursor protein and components of insulin signaling pathways highlighting its significance in maintaining health and preventing disease progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling (PubMed : 10684867, PubMed : 17051221, PubMed : 17613531, PubMed : 18986166, PubMed : 19321446, PubMed : 21098034, PubMed : 2293021, PubMed : 23922390, PubMed : 24847884, PubMed : 26394692, PubMed : 26968463, PubMed : 29596046). Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin (PubMed : 23922390, PubMed : 26394692, PubMed : 29596046). Contributes to the regulation of peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP (By similarity). Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia (Probable) (PubMed : 26394692, PubMed : 9830016). Degrades the natriuretic peptides ANP, BNP and CNP, inactivating their ability to raise intracellular cGMP (PubMed : 21098034). Also degrades an aberrant frameshifted 40-residue form of NPPA (fsNPPA) which is associated with familial atrial fibrillation in heterozygous patients (PubMed : 21098034). Involved in antigen processing. Produces both the N terminus and the C terminus of MAGEA3-derived antigenic peptide (EVDPIGHLY) that is presented to cytotoxic T lymphocytes by MHC class I.. (Microbial infection) The membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV).

See full target information IDE

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:1057 PubMed36539418

2022

Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Deng,Rui Duan,Wangli Ding,Qiuchen Gu,Manman Liu,Junshan Zhou,Jianguo Sun,Junrong Zhu

Brain pathology (Zurich, Switzerland) 32:e13032 PubMed34713522

2021

Distinct Aβ pathology in the olfactory bulb and olfactory deficits in a mouse model of Aβ and α-syn co-pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Friesen,Stephanie Ziegler-Waldkirch,Milena Egenolf,Paolo d'Errico,Christina Helm,Charlotte Mezö,Nikolaos Dokalis,Daniel Erny,Natalie Katzmarski,Romina Coelho,Desirée Loreth,Marco Prinz,Melanie Meyer-Luehmann

Behavioural neurology 2021:6683318 PubMed33880134

2021

High Methionine Diet-Induced Alzheimer's Disease like Symptoms Are Accompanied by 5-Methylcytosine Elevated Levels in the Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Pi,Shenjiao Wei,Yongxuan Jiang,Jing-Shan Shi

Acta neuropathologica communications 6:44 PubMed29855361

2018

Environmental enrichment reverses Aβ pathology during pregnancy in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Ziegler-Waldkirch,Karin Marksteiner,Johannes Stoll,Paolo d'Errico,Marina Friesen,Denise Eiler,Lea Neudel,Verena Sturn,Isabel Opper,Moumita Datta,Marco Prinz,Melanie Meyer-Luehmann

View all publications

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重组Anti-Insulin degrading enzyme / IDE抗体[EPR6099]