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AB6995

Anti-Insulin 抗体 [K36aC10]

Anti-Insulin antibody [K36aC10]

4

(9 Reviews)

|

(107 Publications)

Anti-Insulin antibody [K36aC10] (ab6995) is a mouse monoclonal antibody detecting Insulin in IHC-P. Suitable for Human.

- Over 80 publications
- Trusted since 2001

查看别名

Insulin, INS

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [K36aC10] (AB6995)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [K36aC10] (AB6995)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling insulin with ab6995 at 1/25 dilution.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

K36aC10

亚型

IgG1

轻链类型

kappa

不含载体蛋白

No

反应种属

Human

应用

IHC-P

applications

免疫原

Full Length Protein corresponding to Human INS. The exact immunogen used to generate this antibody is proprietary information.

P01308

特异性

The antibody exhibits cross-reactivity with human proinsulin. This antibody recognizes purified insulin from the pancreas of human, bovine, horse, sheep, and proinsulin from human. Cross reaction has been observed with insulin containing cells in fixed sections of pancreas from human, porcine, dog, rabbit, bovine, sheep, rat, guinea pig and cat.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/50", "IHCP-species-notes": "<p></p>" } } }
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产品详情

What is this antibody validated in?
Anti-Insulin antibody [K36aC10] (ab6995) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Trusted by the scientific community
Anti-Insulin [K36aC10] (ab6995) was first used in a scientific publication in 2001 and has been cited over 80 times in peer-reviewed journals.

Reviewed by scientists
Anti-Insulin [K36aC10] (ab6995) has over 5 independent reviews from customers.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A/G
存储溶液
Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.
Biological function summary

Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.

Pathways

Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.

Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
See full target information INS
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文献 (107)

Recent publications for all applications. Explore the full list and refine your search

Nutrition & diabetes 15:36 PubMed40830335

2025

Kefir-derived exopolysaccharide ameliorates hyperglycemic control and beta cell integrity in a rat model of type 2 diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Ching Yen,Chung-Liang Tsai,Gary Ro-Lin Chang,Chi-Hua Ko,Min-Yu Tu,Ying-Wei Lan,Hsiao-Ling Chen,Chuan-Mu Chen

International journal of molecular sciences 26: PubMed40362452

2025

The Beneficial Impact of a Novel Pancreatic Polypeptide Analogue on Islet Cell Lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Wuyun Zhu,Neil Tanday,Peter R Flatt,Nigel Irwin

Stem cell research & therapy 15:458 PubMed39623490

2024

Knockout of B2M in combination with PD-L1 overexpression protects MSC-derived new islet β cells from graft rejection in the treatment of canine diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Pengxiu Dai,Yi Wu,Qingjie Du,Juanjuan Du,Keyi Wang,Ruiqi Chen,Xiancheng Feng,Chen Chen,Xinke Zhang

PloS one 19:e0311761 PubMed39388496

2024

CRISPR/Cas9 gene editing in induced pluripotent stem cells to investigate the feline hypertrophic cardiomyopathy causing MYBPC3/R820W mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Luke C Dutton,Jayesh Dudhia,Deborah J Guest,David J Connolly

Diabetic medicine : a journal of the British Diabetic Association 42:e15434 PubMed39255356

2024

Cafeteria diet compromises natural adaptations of islet cell transdifferentiation and turnover in pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Vaibhav Dubey,Neil Tanday,Nigel Irwin,Andrei I Tarasov,Peter R Flatt,R Charlotte Moffett

Diabetes, obesity & metabolism 26:4945-4957 PubMed39192525

2024

[P]PP, a stable, long-acting pancreatic polypeptide analogue, evokes weight lowering and pancreatic beta-cell-protective effects in obesity-associated diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Neil Tanday,Wuyun Zhu,Andrei I Tarasov,Peter R Flatt,Nigel Irwin

Peptides 178:171254 PubMed38815655

2024

Chronic exposure to incretin metabolites GLP-1(9-36) and GIP(3-42) affect islet morphology and beta cell health in high fat fed mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ananyaa Sridhar,Dawood Khan,Gayathri Babu,Nigel Irwin,Victor A Gault,Peter R Flatt,Charlotte R Moffett

The Journal of pharmacy and pharmacology 76:861-872 PubMed38652540

2024

Dopamine signalling in pancreatic islet cells and role in adaptations to metabolic stress.

Applications

Unspecified application

Species

Unspecified reactive species

Ananyaa Sridhar,Peter R Flatt,Matthew Draper,Andrei I Tarasov,R Charlotte Moffett,Nigel Irwin,Dawood Khan

European journal of pharmacology 962:176215 PubMed38056618

2023

OXM-104, a potential candidate for the treatment of obesity, NASH and type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Simone Anna Melander,Ashref Kayed,Kim Vietz Andreassen,Morten Asser Karsdal,Kim Henriksen

Diabetes, obesity & metabolism 26:329-338 PubMed37818589

2023

Sustained metabolic benefits of ΔTRTX-Ac1, a tarantula venom-derived peptide, when administered together with exenatide in high-fat fed mice.

Applications

Unspecified application

Species

Unspecified reactive species

Aimee Coulter-Parkhill,Neil Tanday,Diego Cobice,Christopher M McLaughlin,Stephen McClean,Victor A Gault,Nigel Irwin
View all publications
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