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Signal Transduction Growth Factors/Hormones Insulin / Insulin-like

Anti-Insulin + Proinsulin抗体[D6C4] (ab8304)

  • Datasheet
  • SDS
Reviews (6)Q&A (30)References (34)

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Key features and details

  • Mouse monoclonal [D6C4] to Insulin + Proinsulin
  • Suitable for: ELISA
  • Reacts with: Mouse, Rat, Cow, Human, Pig
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Insulin + Proinsulin抗体[D6C4]
    参阅全部 Insulin + Proinsulin 一抗
  • 描述

    小鼠单克隆抗体[D6C4] to Insulin + Proinsulin
  • 宿主

    Mouse
  • 特异性

    Specific for insulin and proinsulin.

  • 经测试应用

    适用于: ELISAmore details
  • 种属反应性

    与反应: Mouse, Rat, Cow, Human, Pig
  • 免疫原

    Human insulin.

  • 常规说明

    Kd for this antibody is 8.1 x 10-8M.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituent: 99.91% PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • Primary antibody说明

    Detection of both insulin and proinsulin.
  • 克隆

    单克隆
  • 克隆编号

    D6C4
  • 骨髓瘤

    unknown
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Signal Transduction
    • Growth Factors/Hormones
    • Insulin / Insulin-like
    • Neuroscience
    • Neurology process
    • Metabolism
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

相关产品

  • Affibody® Molecule

    • Anti-Insulin Affibody® Molecule (ab31906)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • sELISA pair antibody

    • HRP Anti-Insulin + Proinsulin antibody [D3E7] (ab28063)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab8304于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA (1)
Use a concentration of 10 µg/ml.
说明
ELISA
Use a concentration of 10 µg/ml.

靶标

  • 相关性

    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Defects in insulin are the cause of familial hyperproinsulinemia.
  • 细胞定位

    Secreted
  • 数据库链接

    • Entrez Gene: 280829 Cow
    • Entrez Gene: 3630 Human
    • Entrez Gene: 16333 Mouse
    • Entrez Gene: 16334 Mouse
    • Entrez Gene: 24505 Rat
    • Omim: 176730 Human
    • SwissProt: P01317 Cow
    • SwissProt: P01308 Human
    • SwissProt: P01315 Pig
    see all
  • 别名

    • ILPR antibody
    • ins antibody
    • Insulin antibody
    • IRDN antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all

实验方案

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (34)

发表研究结果有使用 ab8304?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab8304 被引用在 34 文献中.

  • Hameedat F  et al. Functionalized zein nanoparticles targeting neonatal Fc receptor to enhance lung absorption of peptides. Drug Deliv Transl Res 13:1699-1715 (2023). PubMed: 36587110
  • Southam K  et al. Development and characterisation of a rat model that exhibits both metabolic dysfunction and neurodegeneration seen in type 2 diabetes. J Physiol 600:1611-1630 (2022). PubMed: 35128667
  • Ramin-Mangata S  et al. Effects of proprotein convertase subtilisin kexin type 9 modulation in human pancreatic beta cells function. Atherosclerosis 326:47-55 (2021). PubMed: 33933263
  • Huang R  et al. Retinol-Binding Protein 4 Activates STRA6, Provoking Pancreatic ß-Cell Dysfunction in Type 2 Diabetes. Diabetes 70:449-463 (2021). PubMed: 33199363
  • Suthamwong P  et al. Administration of mulberry leaves maintains pancreatic ß-cell mass in obese/type 2 diabetes mellitus mouse model. BMC Complement Med Ther 20:136 (2020). PubMed: 32375753
View all Publications for this product

客户评价及客户问答

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提交评价 提交问题

1-10 of 36 Abreviews or Q&A

ELISA abreview for Anti-Insulin + Proinsulin antibody [D6C4]

Excellent
Abreviews
Abreviews
abreview image
Application
ELISA
Sample
Bos taurus Serum (Blood plasma (EDTA))
Specification
Blood plasma (EDTA)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Type
Sandwich (Detection)
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Julie Kim

Verified customer

提交于 Nov 17 2018

Western blot abreview for Anti-Insulin + Proinsulin antibody [D6C4]

Poor
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (pancreatic islet cells)
Loading amount
50 µg
Specification
pancreatic islet cells
Gel Running Conditions
Non-reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Sarah Wang

Verified customer

提交于 May 15 2013

Immunoprecipitation abreview for Anti-Insulin + Proinsulin antibody [D6C4]

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (MIN6 cell line)
Total protein in input
600 µg
Specification
MIN6 cell line
Treatment
RIPA buffer with SDS for 5 min
Immuno-precipitation step
Protein A
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Yuichi Tsuchiya

Verified customer

提交于 Jul 05 2012

Question

ab100578 chez le rat?

Read More

Abcam community

Verified customer

Asked on Feb 12 2013

Answer



Les anticorps utilisés dans le kit ELISA ab100578 sont les anticorps ab8304 comme anticorps de capture et ab20756 comme anticorps de détection. Ces deux anticorps sont testés et garantis chez le rat. Le kit lui-même n’a pas été testé chez le rat mais il y’a des chances que cela fonctionne. Si vous souhaitez le tester, je peux vous faire une offre de test.

Read More

Abcam Scientific Support

回复于 Feb 12 2013

Question

Thank you very much for your information.

Without a picture, I am a little worried whether it will work on Western blot or not. Since this is a kind of expensive antibody, I am wondering if we can have a small aliquot (couple of micro letters?) to try this out. If we get positive result, we will order a regular size and our data can be used as proof for further sales for Abcam.

Read More

Abcam community

Verified customer

Asked on Nov 28 2012

Answer

I am very pleased to hear you would like to use ab8304 in WB and send us an image of your results.

This code will give you $367 off your next order before the expiration date. To redeem this offer, please submit an Abreview with the image for WB and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

Read More

Abcam Scientific Support

回复于 Nov 28 2012

Question

Please send an image of a western blot stained with this antibody.

Read More

Abcam community

Verified customer

Asked on Nov 28 2012

Answer

Thank you for contacting us. I found in our records a note stating that western blotting was added to the list of tested applications for the insulin/pro-insulin antibody ab8304 based on a single report from a customer. Unfortunately, the customer did not send an image of their blot. They briefly described their protocol as follows:

Dilution: 1/2000. Use under non reducing condition. A customer reported successfully staining a nitrocellulose blot of mammalian cell culture media transferred from a non-reducing SDS-PAGE gel. The blot was blocked with 4% skim milk in PBS.

Please let me know if you have any questions.

Read More

Abcam Scientific Support

回复于 Nov 28 2012

Question

Dear technical team,

Our customerwould like to receive CoC of below antibodies.

1. ab8304 (Lot# GR81194-1)
2. ab28063 (Lot#GR58294-1)

Please send me the files.
Thank you in advance.

Best regards

Read More

Abcam community

Verified customer

Asked on Aug 23 2012

Answer

Thank you for your enquiry.



Please find enclosed the requested documents as attachments.



If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Abcam Scientific Support

回复于 Aug 23 2012

Question

Have you tested your human insulin assay against the insulin analogs, such as humalog (lispro), novolog (aspart), lantus (glarginine). If so what is the cross-reactivity to these analogs.

Read More

Abcam community

Verified customer

Asked on Jul 09 2012

Answer

Thank you for your inquiry. 
The antibody pair that we use in the Human Insulin ELISA Kit (ab100578) is capture antibody (ab8304) and detection antibody (ab20756). 
https://www.abcam.com/insulin--proinsulin-antibody-d6c4-ab8304.html
https://www.abcam.com/insulin-proinsulin-antibody-d3e7-biotin-ab20756.html
We have not specifically tested human analogs with this kit but based on sequence similarity, we would expect the kit to recognize these as well.  However, we wouldn't guarantee it since it hasn't been specifically tested.
I hope this information helps. Please contact us with any other questions.

Read More

Abcam Scientific Support

回复于 Jul 09 2012

Question

It ist possible to get the Anti-Insulin + Proinsulin antibody [D6C4] (ab8304) as biotin coated?

Read More

Abcam community

Verified customer

Asked on Jun 06 2012

Answer

Thank you for your patience. My colleague has just added the biotinylated form of this clone to our catalog as ab133958. I hope this helps, please let me know if you need any additional assistance.

Read More

Abcam Scientific Support

回复于 Jun 06 2012

Question

it ist possible to get the Anti-Insulin + Proinsulin antibody [D6C4] (ab8304) as biotin coated?

Read More

Abcam community

Verified customer

Asked on Jun 04 2012

Answer

Thank you for contacting us.

While we do not currently have a biotin conjugated form of ab8304 in our catalog, I am looking into the possibility of adding one for you. I will let you know as soon as I have any information about whether this will be possible or not. We do have another Insulin + Proinsulin antibody that is already biotinylated: ab20756. Alternatively, if you would like to biotin conjugated ab8304, we do sell conjugation kits that would be suitable for that purpose, such as ab102867. I hope this helps, please let me know if you need any additional information or assistance and I will be happy to help you further.

Read More

Abcam Scientific Support

回复于 Jun 04 2012

1-10 of 36 Abreviews or Q&A

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