重组Anti-INSL3抗体[EPR20747] (ab224643)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20747] (ab224643)](https://www.abcam.cn/ps/products/224/ab224643/Images/ab224643-292992-224643IHCa.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20747] (ab224643)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20747] to INSL3
- Suitable for: IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-INSL3抗体[EPR20747]
参阅全部 INSL3 一抗 -
描述
兔单克隆抗体[EPR20747] to INSL3 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- IHC-P: Human testis, ovary and Leydig tumor tissues. IP: Human testis lysate.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20747 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224643于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| IP |
1/30.
|
| 说明 |
|---|
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/30. |
靶标
-
功能
Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor. -
组织特异性
Expressed in prenatal and postnatal Leydig cells. Found as well in the corpus luteum, trophoblast, fetal membranes and breast. -
疾病相关
Defects in INSL3 seems to be a cause of cryptorchidism (CRYPTO) [MIM:219050]; also known as impaired testicular descent. It is one of the most frequent congenital abnormalities in humans, involving 2-5% of male births. Cryptorchidism is associated with increased risk of infertility and testicular cancer. The frequency of INSL3 gene mutations as a cause of cryptorchidism is low. -
序列相似性
Belongs to the insulin family. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 3640 Human
- Omim: 146738 Human
- SwissProt: P51460 Human
- Unigene: 37062 Human
-
别名
- INSL 3 antibody
- INSL3 antibody
- INSL3_HUMAN antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20747] (ab224643)
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling INSL3 with ab224643 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in Leydig cells of human testis (PMID: 19329805). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20747] (ab224643)](https://www.abcam.cn/ps/products/224/ab224643/Images/ab224643-292991-224643IHCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20747] (ab224643)Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling INSL3 with ab224643 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in thecal cells of human ovary (PMID: 21138583). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20747] (ab224643)](https://www.abcam.cn/ps/products/224/ab224643/Images/ab224643-292990-224643IHCc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20747] (ab224643)Immunohistochemical analysis of paraffin-embedded human Leydig tumor tissue labeling INSL3 with ab224643 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human Leydig tumor cells (PMID: 25609776). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunoprecipitation - Anti-INSL3 antibody [EPR20747] (ab224643)](https://www.abcam.cn/ps/products/224/ab224643/Images/ab224643-292988-224643IP.jpg)
Immunoprecipitation - Anti-INSL3 antibody [EPR20747] (ab224643)INSL3 was immunoprecipitated from 0.35 mg of human testis lysate with ab224643 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224643 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Human testis lysate 10 µg (Input).
Lane 2: ab224643 IP in human testis lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224643 in human testis lysate.
Exposure time: 3 seconds.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
-
![Anti-INSL3 antibody [EPR20747] (ab224643)](https://www.abcam.cn/ps/products/224/ab224643/Images/ab224643-5-benefits-of-recombinant-antibodies.png)
Anti-INSL3 antibody [EPR20747] (ab224643)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (0)
ab224643 尚未被引用在任何文献中。