AB227974

重组Anti-INSL3抗体[EPR20739]

Name: INSL3抗体[EPR20739] (ab227974)| Abcam中文官网
Brand: Abcam Limited
SKU: AB227974
Price: 2660 CNY
Availability: InStock
Rating: 1 (3 reviews)

Anti-INSL3 antibody [EPR20739]

RabMAb
Recombinant
了解详情

(3 Reviews)

(1 Publication)

Rabbit Recombinant Monoclonal INSL3 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

查看别名

RLF, RLNL, INSL3, Insulin-like 3, Leydig insulin-like peptide, Relaxin-like factor, Ley-I-L

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20739] (AB227974)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20739] (AB227974)

Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling INSL3 with ab227974 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in thecal cells of human ovary is observed (PMID : 21138583). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20739] (AB227974)

Immunohistochemical analysis of paraffin-embedded human Leydig tumor tissue labeling INSL3 with ab227974 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human Leydig tumor cells is observed (PMID : 25609776). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20739] (AB227974)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling INSL3 with ab227974 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in Leydig cells of human testis is observed (PMID : 19329805). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-INSL3 antibody [EPR20739] (AB227974)

INSL3 was immunoprecipitated from 0.35 mg of human testis lysate with ab227974 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227974 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human testis lysate 10 μg (Input).

Lane 2 : ab227974 IP in human testis lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab227974 in human testis lysate.

Exposure time : 10 seconds.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-INSL3 antibody [EPR20739] (ab227974)

Predicted band size: 14 kDa

Observed band size: 15 kDa,30 kDa

true

Supplier Data

Western blot - Anti-INSL3 antibody [EPR20739] (AB227974)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-INSL3 antibody [EPR20739] (ab227974) at 1/10000 dilution

All lanes:

Human testis lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution

Predicted band size: 14 kDa

Observed band size: 15 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-INSL3 antibody [EPR20739] (AB227974)

Bocking/Dilution buffer : 5% NFDM/TBST.

The 30kDa band is a dimer.

All lanes:

Western blot - Anti-INSL3 antibody [EPR20739] (ab227974) at 1/1000 dilution

Lane 1:

Untreated human testis lysate at 10 µg

Lane 2:

Human testis lysate treated with double DTT (100 mmol/L) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 14 kDa

Observed band size: 15 kDa,30 kDa

true

Exposure time: 1s

不同偶联物与剂型 (1)

Carrier free

Anti-INSL3 antibody [EPR20739] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR20739

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This product recognises a different epitope compared to ab224643.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The INSL3 gene encodes insulin-like peptide 3 also known as Relaxin-like peptide. It is a hormone belonging to the insulin superfamily with a molecular mass of approximately 12 kDa. INSL3 is expressed primarily in the Leydig cells of the testes in males but can also be found in the theca cells of the ovaries in females. This peptide hormone plays a significant role in male and female reproductive physiology by acting through its receptor RXFP2 (Relaxin family peptide receptor 2).

Biological function summary

Insulin-like peptide 3 influences the development of male gonads and the descent of testes during embryonic development making it important for reproductive success. INSL3 also functions in ovarian follicle maturation in females. This hormone operates by binding to the RXFP2 receptor forming a complex that triggers signaling cascades leading to tissue development and differentiation. However the extent of its involvement in female reproductive processes remains under investigation.

Pathways

Insulin-like peptide 3 functions mainly through the cAMP signaling pathway via its interaction with RXFP2 which plays an important role in regulating cell proliferation and differentiation. INSL3 influences the reproductive hormone axis working alongside other hormones like testosterone and luteinizing hormone to coordinate testicular descent and manage reproductive function. Its signaling pathway impacts various stages of gonadal development and maturation.

Mutations or dysfunctions in the INSL3 or RXFP2 gene may result in cryptorchidism a condition where one or both testicles fail to descend properly affecting fertility. This condition involves the hormone-testosterone pathway highlighting the critical interactions between INSL3 and testosterone synthesis. Additionally altered levels of INSL3 could be linked to conditions related to ovarian function disorders reflecting its importance in both male and female reproductive health.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor.

See full target information INSL3

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37175979

2023

The Air-Liquid Interface Reorganizes Membrane Lipids and Enhances the Recruitment of Slc26a3 to Lipid-Rich Domains in Human Colonoid Monolayers.

Applications

Unspecified application

Species

Unspecified reactive species

C Ming Tse,Zixin Zhang,Ruxian Lin,Rafiquel Sarker,Mark Donowitz,Varsha Singh

View all publications

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重组Anti-INSL3抗体[EPR20739]