重组Anti-INPP4A抗体[EP3425(2)] (ab109622)
![Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)](https://www.abcam.cn/ps/products/109/ab109622/Images/ab109622-269757-anti-inpp4a-antibody-ep34252-western-blot.jpg "Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3425(2)] to INPP4A
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
-
产品名称
Anti-INPP4A抗体[EP3425(2)]
参阅全部 INPP4A 一抗 -
描述
兔单克隆抗体[EP3425(2)] to INPP4A -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Fetal brain and Jurkat cell lysates. Human brain tissue.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP3425(2) -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109622于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 104 kDa (predicted molecular weight: 110 kDa).
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
(Heat to 98°C, allow to cool for 10-20 minutes) |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 104 kDa (predicted molecular weight: 110 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) |
靶标
-
功能
Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-bisphosphate. -
组织特异性
Isoform 1 is expressed in the platelets, MEG-01 megakaryocytes and Jurkat T-cells. Isoform 2 is expressed in the brain. -
通路
Signal transduction; phosphatidylinositol signaling pathway. -
序列相似性
Belongs to the inositol-3,4-bisphosphate 4-phosphatase family.
Contains 1 C2 domain. - Information by UniProt
-
数据库链接
- Entrez Gene: 3631 Human
- Entrez Gene: 269180 Mouse
- Entrez Gene: 80849 Rat
- Omim: 600916 Human
- SwissProt: Q96PE3 Human
- SwissProt: Q9EPW0 Mouse
- SwissProt: Q62784 Rat
- Unigene: 469386 Human
see all -
别名
- 107kDa antibody
- 4-bisphosphate 4-phosphatase antibody
- 9630012D15 antibody
see all
图片
-
Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: INPP4A knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: Human fœtal brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109622 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.ab109622 was shown to specifically react with INPP4A when INPP4A knockout samples were used, along with additional cross-reactive bands. Wild-type and INPP4A knockout samples were subjected to SDS-PAGE. Ab109622 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) andGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)](https://www.abcam.cn/ps/products/109/ab109622/Images/ab109622-122567-anti-inpp4a-antibody-ep34252-western-blot.jpg)
Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)All lanes : Anti-INPP4A antibody [EP3425(2)] (ab109622) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 110 kDa
Observed band size: 104 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4A antibody [EP3425(2)] (ab109622)](https://www.abcam.cn/ps/products/109/ab109622/Images/ab109622-122566-anti-inpp4a-antibody-ep34252-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4A antibody [EP3425(2)] (ab109622)ab109622 at 1/100 dilution staining INPP4A in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Anti-INPP4A antibody [EP3425(2)] (ab109622)](https://www.abcam.cn/ps/products/109/ab109622/Images/ab109622-4-benefits-of-recombinant-antibodies.png)
Anti-INPP4A antibody [EP3425(2)] (ab109622)
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab109622 被引用在 3 文献中.
- Iguchi A et al. INPP5D modulates TREM2 loss-of-function phenotypes in a β-amyloidosis mouse model. iScience 26:106375 (2023). PubMed: 37035000
- Wang H et al. Phosphatidylinositol 3,4-bisphosphate synthesis and turnover are spatially segregated in the endocytic pathway. J Biol Chem 295:1091-1104 (2020). PubMed: 31831620
- Daste F et al. Control of actin polymerization via the coincidence of phosphoinositides and high membrane curvature. J Cell Biol 216:3745-3765 (2017). PubMed: 28923975