AB109622

重组Anti-INPP4A抗体[EP3425(2)]

Name: INPP4A抗体[EP3425(2)] (ab109622)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109622
Price: 2410 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-INPP4A antibody [EP3425(2)]

RabMAb
Recombinant
KO Validated
了解详情

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal INPP4A antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 4 publications.

查看别名

Inositol polyphosphate-4-phosphatase type I A, Inositol polyphosphate 4-phosphatase type I, INPP4A

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4A antibody [EP3425(2)] (AB109622)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4A antibody [EP3425(2)] (AB109622)

ab109622 at 1/100 dilution staining INPP4A in paraffin-embedded Human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Western blot - Anti-INPP4A antibody [EP3425(2)] (AB109622)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : INPP4A knockout HAP1 cell lysate (20 μg)
Lane 3 : Jurkat cell lysate (20 μg)
Lane 4 : Human fœtal brain tissue lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab109622 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab109622 was shown to specifically react with INPP4A when INPP4A knockout samples were used, along with additional cross-reactive bands. Wild-type and INPP4A knockout samples were subjected to SDS-PAGE. ab109622 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) andGoat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622)

Predicted band size: 110 kDa

false

Lab

Western blot - Anti-INPP4A antibody [EP3425(2)] (AB109622)

Western blot : Rabbit Monoclonal[EP3425(2)] to INPP4A ab109622 staining at 1/500 dilution, shown in green; Mouse anti alpha Tubulin (ab7291) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 110 kDa in Wild-type A549 cell lysates with no signal observed at this size in INPP4A knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622) at 1/500 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

INPP4A knockout A549 at 20 µg

Lane 3:

HL-60 at 20 µg

Lane 4:

MCF7 at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 110 kDa

Observed band size: 110 kDa,50 kDa

false

Unknown

Western blot - Anti-INPP4A antibody [EP3425(2)] (AB109622)

All lanes:

Western blot - Anti-INPP4A antibody [EP3425(2)] (ab109622) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 110 kDa

Observed band size: 104 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP3425(2)

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "(Heat to 98°C, allow to cool for 10-20 minutes)", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "(Heat to 98°C, allow to cool for 10-20 minutes)", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

INPP4A also known as inositol polyphosphate 4-phosphatase type I is an enzyme that specifically dephosphorylates the D-4 position phosphate of phosphoinositides. It has a molecular mass of about 105 kDa. This enzyme is expressed in several tissues including brain and platelets indicating its involvement in various cellular processes. INPP4A is significant in controlling the intracellular levels of phosphoinositide messengers and plays an important role in signaling pathways.

Biological function summary

INPP4A functions to regulate phosphoinositide signaling which is critical in controlling cell growth apoptosis and other cellular processes. It acts by reducing levels of phosphatidylinositol 34-bisphosphate an important lipid signaling molecule. INPP4A does not appear to form part of a larger complex but operates within a network of other phosphatases and kinases that together maintain lipid signaling homeostasis.

Pathways

INPP4A interacts mainly in the phosphoinositide 3-kinase (PI3K) signaling pathway. This pathway is important for various cellular functions such as metabolism cell growth and survival. INPP4A modifies the activity of related proteins within this pathway like PI3K by reducing phosphatidylinositol 34-bisphosphate levels therefore controlling downstream signaling. It also connects with other phosphoinositide phosphatases like PTEN to finely tune the signaling balance.

INPP4A is linked to neurological disorders and cancer. In cancer the dysfunction of INPP4A can lead to abnormal cell growth due to dysregulated PI3K signaling. This enzyme's activity is central in maintaining normal phosphoinositide turnover and its interactions with proteins such as PTEN underline its importance in preventing uncontrolled cell proliferation. For neurological disorders alterations in INPP4A function may affect synapse function and neuronal survival contributing to neurodegenerative processes.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) (PubMed : 15716355, PubMed : 20463662). Catalyzes also inositol 1,3,4-trisphosphate and inositol 1,4-bisphosphate (By similarity). Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival (By similarity) (PubMed : 30071275). May protect neurons from excitotoxic cell death by regulating the synaptic localization of cell surface N-methyl-D-aspartate-type glutamate receptors (NMDARs) and NMDAR-mediated excitatory postsynaptic current (By similarity).. Isoform 4. Displays no 4-phosphatase activity for PtdIns(3,4)P2, Ins(3,4)P2, or Ins(1,3,4)P3.

See full target information Inositol polyphosphate-4-phosphatase type I A

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106375 PubMed37035000

2023

INPP5D modulates TREM2 loss-of-function phenotypes in a β-amyloidosis mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Akihiro Iguchi,Sho Takatori,Shingo Kimura,Hiroki Muneto,Kai Wang,Hayato Etani,Genta Ito,Haruaki Sato,Yukiko Hori,Junko Sasaki,Takashi Saito,Takaomi C Saido,Tsuneya Ikezu,Toshiyuki Takai,Takehiko Sasaki,Taisuke Tomita

Anticancer research 40:6101-6113 PubMed33109548

2020

miR-935 Inhibits Oral Squamous Cell Carcinoma and Targets Inositol Polyphosphate-4-phosphatase Type IA (INPP4A).

Applications

Unspecified application

Species

Unspecified reactive species

Nobuyuki Maruyama,Masato Umikawa,Hirofumi Matsumoto,Tessho Maruyama,Kazuhide Nishihara,Toshiyuki Nakasone,Akira Matayoshi,Takahiro Goto,Fusahiro Hirano,Akira Arasaki,Hiroyuki Nakamura,Goro Matsuzaki,Giichi Takaesu

The Journal of biological chemistry 295:1091-1104 PubMed31831620

2019

Phosphatidylinositol 3,4-bisphosphate synthesis and turnover are spatially segregated in the endocytic pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Haibin Wang,Dinah Loerke,Caroline Bruns,Rainer Müller,Philipp-Alexander Koch,Dmytro Puchkov,Carsten Schultz,Volker Haucke

The Journal of cell biology 216:3745-3765 PubMed28923975

2017

Control of actin polymerization via the coincidence of phosphoinositides and high membrane curvature.

Applications

Unspecified application

Species

Unspecified reactive species

Frederic Daste,Astrid Walrant,Mikkel R Holst,Jonathan R Gadsby,Julia Mason,Ji-Eun Lee,Daniel Brook,Marcel Mettlen,Elin Larsson,Steven F Lee,Richard Lundmark,Jennifer L Gallop

View all publications

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For licensing inquiries, please contact partnerships@abcam.com

重组Anti-INPP4A抗体[EP3425(2)]

Anti-INPP4A antibody [EP3425(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4A antibody [EP3425(2)] (AB109622)

Western blot - Anti-INPP4A antibody [EP3425(2)] (AB109622)

Western blot - Anti-INPP4A antibody [EP3425(2)] (AB109622)

Western blot - Anti-INPP4A antibody [EP3425(2)] (AB109622)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原