Name: Anti-iNOS antibody [NOS-IN]
SKU: ab49999
Rating: 4 (5 reviews)

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Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [NOS-IN] (ab49999)

Key features and details

Mouse monoclonal [NOS-IN] to iNOS
Suitable for: ICC/IF, WB
Reacts with: Mouse
Isotype: IgG1

产品名称

Anti-iNOS抗体[NOS-IN]
参阅全部 iNOS 一抗
描述

小鼠单克隆抗体[NOS-IN] to iNOS
宿主

Mouse
特异性

This antibody reacts specifically with inducible-type nitricoxide synthase (iNOS, also termed macNOS and mNOS). It does not react with NOS derived from brain.
经测试应用

适用于: ICC/IF, WBmore details
种属反应性

与反应: Mouse
免疫原

Synthetic peptide corresponding to Mouse iNOS aa 1126-1144 conjugated to keyhole limpet haemocyanin.
Sequence:
FSYGAKKGSALEEPKATRL

Run BLAST with Run BLAST with
阳性对照
- WB: Whole cell extract of RAW 264.7 activated with LPS and IFN-gamma. ICC/IF: RAW 264.7 activated with LPS and IFN-gamma.
常规说明

This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.4
Preservative: 0.087% Sodium azide
Constituent: 2.93% PBS
Concentration information loading...
纯化说明

This antibody is purified from hybridoma cell culture.
克隆

单克隆
克隆编号

NOS-IN
同种型

IgG1
研究领域

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
ELISA pair antibody
- Anti-iNOS antibody [EPR16635] (ab178945)
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
Positive Controls
- RAW 264.7 whole cell lysate (ab7187)
Recombinant Protein
- Recombinant Human iNOS protein (ab135010)

The Abpromise guarantee

Abpromise™承诺保证使用ab49999于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
ICC/IF		Use at an assay dependent concentration.
WB	(4)	Use at an assay dependent concentration. Predicted molecular weight: 131 kDa.

说明
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 131 kDa.

功能

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
组织特异性

Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
序列相似性

Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Target information above from: UniProt accession P35228 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 18126 Mouse
- SwissProt: P29477 Mouse
- Unigene: 2893 Mouse
别名
- HEP-NOS antibody
- Hepatocyte NOS antibody
- HEPNOS antibody
- inducible antibody
- Inducible nitric oxide synthase antibody
- Inducible NO synthase antibody
- Inducible NOS antibody
- iNOS antibody
- MAC NOS antibody
- Macrophage NOS antibody
- Nitric oxide synthase 2 inducible antibody
- Nitric oxide synthase 2 inducible macrophage antibody
- nitric oxide synthase 2A (inducible, hepatocytes) antibody
- Nitric oxide synthase antibody
- Nitric oxide synthase inducible antibody
- nitric oxide synthase, macrophage antibody
- NOS 2 antibody
- NOS antibody
- Nos II antibody
- NOS type II antibody
- nos2 antibody
- NOS2_HUMAN antibody
- NOS2A antibody
- NOS2A, Inducible, Hepatocyte antibody
- Peptidyl-cysteine S-nitrosylase NOS2 antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [NOS-IN] (ab49999)

Immunocytochemistry/ Immunofluorescence analysis of IFN-γ/LPS-activated RAW-264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling iNOS with ab49999 at 1/2000 dilution.

Cells were fixed and permeabilized with 4% paraformaldehyde and 0.5% Triton™ X-100. The antibody was developed using Goat anti-Mouse IgG, Cy3-conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

This image was generated using the ascites version of the product.
Western blot - Anti-iNOS antibody [NOS-IN] (ab49999)

Lane 1 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/500 dilution
Lane 2 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/1000 dilution
Lane 3 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/2500 dilution
Lane 4 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/5000 dilution

All lanes : IFN-gamma/LPS-activated RAW-264.7 cell extract

Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase and chemiluminescent substrate

Predicted band size: 131 kDa

This image was generated using the ascites version of the product.

Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab49999？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab49999 被引用在 79 文献中.

Ma Y et al. Photobiomodulation Attenuates Neurotoxic Polarization of Macrophages by Inhibiting the Notch1-HIF-1α/NF-κB Signalling Pathway in Mice With Spinal Cord Injury. Front Immunol 13:816952 (2022). PubMed: 35371065
Fan Z et al. Intestinal Flora Changes Induced by a High-Fat Diet Promote Activation of Primordial Follicles through Macrophage Infiltration and Inflammatory Factor Secretion in Mouse Ovaries. Int J Mol Sci 23:N/A (2022). PubMed: 35563189
Fan H et al. Exosomes derived from olfactory ensheathing cells provided neuroprotection for spinal cord injury by switching the phenotype of macrophages/microglia. Bioeng Transl Med 7:e10287 (2022). PubMed: 35600663
Hang Z et al. Iguratimod Attenuates Macrophage Polarization and Antibody-Mediated Rejection After Renal Transplant by Regulating KLF4. Front Pharmacol 13:865363 (2022). PubMed: 35614941
Carrascal L et al. Acute Colon Inflammation Triggers Primary Motor Cortex Glial Activation, Neuroinflammation, Neuronal Hyperexcitability, and Motor Coordination Deficits. Int J Mol Sci 23:N/A (2022). PubMed: 35628158

View all Publications for this product

客户评价及客户问答

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提交评价提交问题

1-7 of 7 Abreviews or Q&A

Application

Western blot

Sample

Rat Tissue lysate - whole (Skeletal muscle)

Gel Running Conditions

Reduced Denaturing (8%)

Loading amount

20 µg

Specification

Skeletal muscle

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 09 2016

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (10% gel)

Sample

Rat Cell lysate - whole cell (Skeletal muscle)

Specification

Skeletal muscle

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 27 2015

Application

Western blot

Loading amount

50 µg

Gel Running Conditions

Reduced Denaturing (8% Gel)

Sample

Mouse Tissue lysate - whole (Skeletal muscle)

Specification

Skeletal muscle

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 09 2014

Application

Western blot

Sample

Mouse Tissue lysate - whole (GTN, Skeletal muscle)

Loading amount

50 µg

Specification

GTN, Skeletal muscle

Gel Running Conditions

Reduced Denaturing (8%)

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. G.K Sakellariou

Verified customer

提交于 Jul 05 2012

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Cerebral sections)

Specification

Cerebral sections

Fixative

Formaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid

Permeabilization

Blocking step

glycine as blocking agent for 15 minute(s) · Concentration: 1%

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Charlotte Dmello

Verified customer

提交于 Aug 25 2010

Question

Dear technical support team:

This customer has highly interested in Anti-iNOS antibody [NOS-IN] (ab49999), he would likes to order this antibody and test in WB with porcine sample,

would you please help this customer to create a discount code, the information of this customer as follow:

Could you please help this customer to solve the problem?

Thanks for your kindly help.

Best regards

Abcam community

Verified customer

Asked on Jul 06 2012

Answer

Thank you for your interest.

The immunogen used to raise ab49999 was a synthetic peptide: FSYGAKKGSALEEPKATRL conjugated to KLH, corresponding to amino acids 1126-1144 of Mouse iNOS.

According to blast search the aa homology between rat and mouse iNOS is 94% and we do have cross-reactivity data proving the reactivity with Rattus norvegicus.

However, with human and Macaca monkey the homology is only 80% and we expect hat with pig is is even lower (but no data available).

I regret to inform you that this antibody is very unlikely to detect porcine iNOS so we can't offer a discount code for testing ab49999 in pig.

Your customer may wish to consider ab3523 which was raised suing a different immunogen (DLKEEKDINNNVKKT, corresponding to amino acids 17-31 of Mouse iNOS). It has been tested for cross-reactivity with Mouse, Rat, Chicken, Dog, Human, Xenopus laevis.

If you need any further assistance in the future, please do not hesitate to contact me.

Abcam Scientific Support

回复于 Jul 06 2012

Question

Thank you very much.

The customer prefers to receiveab49999 as a replacement.

Please confirm.

Warm Regards,

Abcam community

Verified customer

Asked on Jan 26 2012

Answer

Thank you for your note.
This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vial of ab49999 (exchange for the original product ab15323) as a free of charge replacement. I hope the second vial will work as it is expected, and please do let me know how you are getting on with this product.
Dear Colleague,
Please add 1 vial of ab49999 to the next shipment for Zotal.
Original CCE ID: 3410854
Original Order: 1003928
Problem is: Non-specific bands in Western blot application
Many thanks.

Abcam Scientific Support

回复于 Jan 26 2012

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Key features and details

概述

产品名称

描述

宿主

特异性

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯化说明

克隆

克隆编号

同种型

研究领域

相关产品

Compatible Secondaries

ELISA pair antibody

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (79)

客户评价及客户问答

Western blot abreview for Anti-iNOS antibody [NOS-IN]

Western blot abreview for Anti-iNOS antibody [NOS-IN]

Western blot abreview for Anti-iNOS antibody [NOS-IN]

Western blot abreview for Anti-iNOS antibody [NOS-IN]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-iNOS antibody [NOS-IN]