Anti-iNOS抗体(ab3523)
Key features and details
- Rabbit polyclonal to iNOS
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-iNOS抗体
参阅全部 iNOS 一抗 -
描述
兔多克隆抗体to iNOS -
宿主
Rabbit -
特异性
This antibody detects iNOS. It does not detect other NOS isoforms. By western blot, this antibody detects an ~135 kDa protein representing recombinant human iNOS. By western blot, this antibody also detects purified recombinant mouse iNOS, mouse iNOS from cytokine stimulated RAW 264.7 cells.
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经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Mouse iNOS aa 1-100.
Database link: P29477 -
阳性对照
- ICC: A549, NIH/3T3 cells. IHC-P: Human heart tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
靶标
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功能
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. -
组织特异性
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. -
序列相似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. - Information by UniProt
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数据库链接
- Entrez Gene: 4843 Human
- Entrez Gene: 18126 Mouse
- Omim: 163730 Human
- SwissProt: P35228 Human
- SwissProt: P29477 Mouse
- Unigene: 709191 Human
- Unigene: 2893 Mouse
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别名
- HEP-NOS antibody
- Hepatocyte NOS antibody
- HEPNOS antibody
see all
图片
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Western blot - Anti-iNOS antibody (ab3523)All lanes : Anti-iNOS antibody (ab3523) at 1/1000 dilution
Lane 1 : RAW264 whole cell lysate untreated
Lane 2 : RAW264 whole cell lysate untreated stimulated with LPS at 1 µg/mL for 16 hours
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, HRP conjugate at 1/1000 dilution
Lane 2 : Goat anti-Rabbit IgG (Heavy Chain) HRP conjugate at 1/1000 dilution
Predicted band size: 131 kDaUsing 4-20% Tris-Glycine polyacrylamide gel and transferred to a nitrocellulose membrane, blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with ab3523 at 4°C overnight on a rocking platform, washed in TBST, and probed with the secondary antibody for 1 hour.
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Western blot - Anti-iNOS antibody (ab3523)All lanes : Anti-iNOS antibody (ab3523) at 1/1000 dilution
Lane 1 : Whole cell lysate of RAW 264.7
Lane 2 : Whole cell lysate of RAW 264.7 treated with LPS
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Predicted band size: 131 kDaThis was run using 4-12% Bis-Tris Protein Gel and a Nitrocellulose membrane.
Observed band at 130kDa in LPS treated RAW 264.7 cells, and an uncharacterized band at ~60kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody (ab3523)Immunohistochemistry analysis of human heart tissue stained for iNOS without (negative control) or using ab3523 at 1/200 dilution overnight at 4°C in a humidified chamber, followed biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor.
To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature.
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Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody (ab3523)Immunofluorescence analysis of A549 (human lung carcinoma cell line) whole cells labelling iNOS (Left panel: green) without (control) or using ab3523 at 1/20 dilution overnight at 4°C, followed DyLight-488 conjugated secondary antibody. Counter stain: F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Cells were grown on chamber slides and fixed with formaldehyde prior to staining.
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Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody (ab3523)Immunofluorescence analysis of NIH/3T3 (mouse embryo fibroblast cell line) whole cells labelling iNOS (Left panel: green) without (control) or using ab3523 at 1/20 dilution overnight at 4°C, followed DyLight-488 conjugated secondary antibody. Counter stain: F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Cells were grown on chamber slides and fixed with formaldehyde prior to staining.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (347)
ab3523 被引用在 347 文献中.
- Ruger L et al. Mechanical High-Intensity Focused Ultrasound (Histotripsy) in Dogs With Spontaneously Occurring Soft Tissue Sarcomas. IEEE Trans Biomed Eng 70:768-779 (2023). PubMed: 36006886
- Sallese A et al. Isolation and Characterization of Peritoneal Macrophages from Salamanders. Methods Mol Biol 2562:259-270 (2023). PubMed: 36272082
- Patino E et al. Iron therapy mitigates chronic kidney disease progression by regulating intracellular iron status of kidney macrophages. JCI Insight 8:N/A (2023). PubMed: 36394951
- Caratti G et al. Macrophagic AMPKα1 orchestrates regenerative inflammation induced by glucocorticoids. EMBO Rep 24:e55363 (2023). PubMed: 36520372
- Pirković A et al. Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells. Antioxidants (Basel) 12:N/A (2023). PubMed: 36671060