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RabMAb

Anti-Inhibin alpha抗体[SP323] (ab224798)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)

Key features and details

  • Rabbit monoclonal [SP323] to Inhibin alpha
  • Suitable for: IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Inhibin alpha抗体[SP323]
    参阅全部 Inhibin alpha 一抗

  • 描述

    兔单克隆抗体[SP323] to Inhibin alpha

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Synthetic peptide corresponding to Human Inhibin alpha (internal sequence).
    Database link: P05111

  • 阳性对照

    • IHC-P: Human stomach carcinoma, testis, adrenal gland, and Mouse testis tissue.

  • 常规说明

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • 纯度

    Protein A/G purified

  • 纯化说明

    Purified from TCS by protein A/G.

  • 克隆

    单克隆

  • 克隆编号

    SP323

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab224798于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/100.

Perform heat mediated antigen retrieval with EDTA pH 8.0 buffer before commencing with IHC staining protocol.
说明
IHC-P
1/100.

Perform heat mediated antigen retrieval with EDTA pH 8.0 buffer before commencing with IHC staining protocol.

靶标

  • 功能

    Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

  • 组织特异性

    Originally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. In adrenal cortex expression is limited to the zona reticularis and the innermost zona fasciculata in the normal gland, extending centripetally into the zona fasciculata in hyperplasia. Also found in adrenocortical tumors. Also expressed in prostate epithelium of benign prostatic hyperplasia, in regions of basal cell hyperplasia and in nonmalignant regions of high grade prostate cancer. Only circulating inhibin B is found in male, whereas circulating inhibins A and B are found in female.

  • 序列相似性

    Belongs to the TGF-beta family.

  • 翻译后修饰

    Proteolytic processing yields a number of bioactive forms. The 20/23 kDa forms consist solely of the mature alpha chain, the 26/29 kDa forms consist of the most N-terminal propeptide linked through a disulfide bond to the mature alpha chain, the 50/53 kDa forms encompass the entire proprotein. Each type can be furthermore either mono- or diglycosylated, causing the mass difference.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • A inhibin subunit antibody
      • A inhibin subunit precursor antibody
      • AW55578 antibody
      • IHA antibody
      • inhA antibody
      • INHA_HUMAN antibody
      • Inhibin alpha chain antibody
      • Inhibin alpha chain precursor antibody
      • Inhibin alpha subunit antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling Inhibin alpha with ab224798 at 1/100 dilution (1.42 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach adenocarcinoma tissue sections labeling Inhibin alpha with ab224798 at 1/100 dilution (1.42 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling Inhibin alpha with ab224798 at 1/100 dilution (1.42 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)

      Formalin-fixed, paraffin-embedded human testis tissue stained for Inhibin alpha using ab224798 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)

      Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Inhibin alpha using ab224798 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] (ab224798)

      Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for Inhibin alpha using ab224798 at 1/100 dilution in immunohistochemical analysis.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (1)

    发表研究结果有使用 ab224798？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab224798 被引用在 1 文献中.

    • Wang J  et al. MitoQ Protects Ovarian Organoids against Oxidative Stress during Oogenesis and Folliculogenesis In Vitro. Int J Mol Sci 24:N/A (2023). PubMed: 36674435

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