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重组

重组Anti-Inhibin alpha抗体[PO12/8] - BSA and Azide free (ab273486)

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Western blot - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)
  • ELISA - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)
  • Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [PO12/8] to Inhibin alpha - BSA and Azide free
  • Suitable for: IHC-P, ELISA, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Inhibin alpha抗体[PO12/8] - BSA and Azide free
    参阅全部 Inhibin alpha 一抗

  • 描述

    小鼠单克隆抗体[PO12/8] to Inhibin alpha - BSA and Azide free

  • 宿主

    Mouse

  • 经测试应用

    适用于: IHC-P, ELISA, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Human testis and placenta tissue lysates. IHC-P: Human testis and ovary tissue. ELISA: Human Inhibin alpha.

  • 常规说明

    ab273486 is the carrier-free version of ab273454.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab273486于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

靶标

  • 功能

    Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

  • 组织特异性

    Originally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. In adrenal cortex expression is limited to the zona reticularis and the innermost zona fasciculata in the normal gland, extending centripetally into the zona fasciculata in hyperplasia. Also found in adrenocortical tumors. Also expressed in prostate epithelium of benign prostatic hyperplasia, in regions of basal cell hyperplasia and in nonmalignant regions of high grade prostate cancer. Only circulating inhibin B is found in male, whereas circulating inhibins A and B are found in female.

  • 序列相似性

    Belongs to the TGF-beta family.

  • 翻译后修饰

    Proteolytic processing yields a number of bioactive forms. The 20/23 kDa forms consist solely of the mature alpha chain, the 26/29 kDa forms consist of the most N-terminal propeptide linked through a disulfide bond to the mature alpha chain, the 50/53 kDa forms encompass the entire proprotein. Each type can be furthermore either mono- or diglycosylated, causing the mass difference.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • A inhibin subunit antibody
      • A inhibin subunit precursor antibody
      • AW55578 antibody
      • IHA antibody
      • inhA antibody
      • INHA_HUMAN antibody
      • Inhibin alpha chain antibody
      • Inhibin alpha chain precursor antibody
      • Inhibin alpha subunit antibody
      see all

    图片

    • Western blot - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)
      Western blot - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)
      All lanes : Anti-Inhibin alpha antibody [PO12/8] (ab273454) at 1/1000 dilution

      Lane 1 : Human testis tissue lysate
      Lane 2 : Human placenta tissue lysate
      Lane 3 : Human tonsil tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Predicted band size: 40 kDa
      Observed band size: 40 kDa



      This data was produced using the same clone but in a different formulation, ab273454.

      Negative control: Human tonsil.
      The molecular weight observed is consistent with what has been described in the literature (PMID:31658054).

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 180 seconds.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)

      This data was produced using the same clone but in a different formulation, ab273454.

      Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling Inhibin alpha with ab273454 at 1/1000 (0.937 µg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplamic staining on the theca interna of the human ovary. The section was incubated with ab273454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • ELISA - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)
      ELISA - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)

      This data was produced using the same clone but in a different formulation, ab273454.

      ab273454 used at 0-2000 ng/ml.

      Antigen (Human Inhibin alpha) used at 1000 ng/ml.

      Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)

      This data was produced using the same clone but in a different formulation, ab273454.

      Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Inhibin alpha with ab273454 at 1/1000 (0.937 µg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplamic staining on human testis. The section was incubated with ab273454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)
      Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab273486？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab273486 尚未被引用在任何文献中。

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