AB300082

重组Anti-ING3抗体[EPR25273-6] (BSA and Azide free)

Anti-ING3 antibody [EPR25273-6] (BSA and Azide free)

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal ING3 antibody. Carrier free. Suitable for WB, IP and reacts with Rat, Mouse, Human samples.

查看别名

HSPC301, ING3, Inhibitor of growth protein 3, p47ING3

5 Images

Supplier Data

Immunoprecipitation - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)

This data was developed using ab300081, the same antibody clone in a different buffer formulation.

ING3 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with ab300081 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300081 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg

Lane 2 : ab300081 in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300081 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 24 seconds

Lysates should be made freshly and used in IP immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-ING3 antibody [EPR25273-6] (<a href='/products/primary-antibodies/ing3-antibody-epr25273-6-ab300081'>ab300081</a>)

Predicted band size: 47 kDa

false

Supplier Data

Immunoprecipitation - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)

This data was developed using ab300081, the same antibody clone in a different buffer formulation.

ING3 was immunoprecipitated from 0.35 mg Mouse testis tissue lysate 10 μg with ab300081 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300081 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse testis tissue lysate 10 μg

Lane 2 : ab300081 in Mouse testis tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300081 in mouse testis tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 50 seconds

All lanes:

Immunoprecipitation - Anti-ING3 antibody [EPR25273-6] (<a href='/products/primary-antibodies/ing3-antibody-epr25273-6-ab300081'>ab300081</a>)

Predicted band size: 47 kDa

false

Supplier Data

Immunoprecipitation - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)

This data was developed using ab300081, the same antibody clone in a different buffer formulation.

ING3 was immunoprecipitated from 0.35 mg Rat testis tissue lysate 10 μg with ab300081 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300081 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat testis tissue lysate 10 μg

Lane 2 : ab300081 in Rat testis tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300081 in Rat testis tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 50 seconds

All lanes:

Immunoprecipitation - Anti-ING3 antibody [EPR25273-6] (<a href='/products/primary-antibodies/ing3-antibody-epr25273-6-ab300081'>ab300081</a>)

Predicted band size: 47 kDa

false

Supplier Data

Western blot - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)

This data was developed using ab300081, the same antibody clone in a different buffer formulation.

5% NFDM/TBST was used as blocking and diluting buffer.

Lysates should be made freshly and used in WB immediately to minimize protein degradation.

All lanes:

Western blot - Anti-ING3 antibody [EPR25273-6] (<a href='/products/primary-antibodies/ing3-antibody-epr25273-6-ab300081'>ab300081</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

Exposure time: 26s

Supplier Data

Western blot - Anti-ING3 antibody [EPR25273-6] (BSA and Azide free) (AB300082)

This data was developed using ab300081, the same antibody clone in a different buffer formulation.

5% NFDM/TBST was used as blocking and diluting buffer.

All lanes:

Western blot - Anti-ING3 antibody [EPR25273-6] (<a href='/products/primary-antibodies/ing3-antibody-epr25273-6-ab300081'>ab300081</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting ING3, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

Exposure time: 59s

不同偶联物与剂型 (1)

Unconjugated

Anti-ING3 antibody [EPR25273-6]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR25273-6

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Mouse, Rat

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The ING3 protein also known as inhibitor of growth family member 3 is a nuclear protein with a molecular mass of approximately 47 kDa. ING3 belongs to the ING gene family and acts as a tumor suppressor by modulating chromatin structure and regulating gene expression. It is expressed in various tissues including the liver kidney and heart and plays a critical role in cellular stress responses. ING3 interacts with several components of the histone acetyltransferase (HAT) complex regulating processes like cell growth apoptosis and DNA repair.

Biological function summary

ING3 influences gene transcription and chromatin remodeling as a part of the NuA4 histone acetyltransferase complex. This complex is involved in the acetylation of histone H4 and H2A facilitating an open chromatin structure that permits transcriptional activation. ING3 is important in the regulation of cell cycle progression and apoptosis. It modulates p53 and other key cellular pathways underpinning its role in maintaining normal cellular homeostasis and preventing cancerous transformations.

Pathways

ING3's function supports significant biological processes like DNA repair and apoptosis. It is part of the p53 signaling pathway which plays a pivotal role in the cellular response to DNA damage. ING3 acts alongside proteins such as p21 and MDM2 in the p53 pathway. Furthermore ING3 interfaces with the MAPK/ERK signaling pathway contributing to its regulation of cell division and survival. Through these pathways ING3 helps orchestrate a balanced response to cellular stress and damage.

Researchers associate ING3 with cancers particularly breast and liver cancers. Studies indicate that downregulation or mutation of ING3 leads to disrupted cell cycle control and increased tumorigenesis. Furthermore ING3's interaction with proteins such as p53 emphasizes its critical role in tumor suppression. By modulating pathways linked to proliferation and apoptosis ING3 provides potential targets for therapeutic intervention in cancer treatments highlighting its relevance in disease management and research.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome.

See full target information ING3

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-ING3抗体[EPR25273-6] (BSA and Azide free)