抗甲型流感Virus Nucleoprotein抗体[C43] (ab128193)
Key features and details
- Mouse monoclonal [C43] to Influenza A Virus Nucleoprotein
- Suitable for: WB, ELISA, ICC/IF, Flow Cyt
- Reacts with: Influenza A
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
抗甲型流感Virus Nucleoprotein抗体[C43]
参阅全部 Influenza A Virus Nucleoprotein 一抗 -
描述
小鼠单克隆抗体[C43] to甲型流感Virus Nucleoprotein -
宿主
Mouse -
特异性
Reacts with NP of all influenza A viruses so far tested, including seasonal H2N2, H3N2(A/Sydney/5/1997), and H5N1(A/crow/Kyoto53/2004), H5N1 (A/duck/Egypt/D2br10/07), H5N1(A/duck/HK/342/78), H5N2(A/crow/Kyoto/53/04), H9N1, H9N2 (A/Turkey/Wisconsin/1/66) and H1N1 (seasonal: A/New Caledonia/20/99. Pandemic: A/Suita/01/2009 and swine: A/PuertoRico/8/34).
No cross reactivity with influenza B viruses.
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经测试应用
适用于: WB, ELISA, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Influenza A -
免疫原
Tissue, cells or virus corresponding to Influenza A Virus Nucleoprotein. Human Influenza A Virus H3N2 strain
Database link: P69291 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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纯度
Proprietary Purification -
纯化说明
Produced in serum-free medium and purified by proprietary chromatography procedure under mild conditions. 90~95% pure by SDS-PAGE -
克隆
单克隆 -
克隆编号
C43 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128193于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/300 - 1/1000.
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
1/200.
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Flow Cyt |
Use at an assay dependent concentration. PubMed: 24067955
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/300 - 1/1000. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
1/200. |
Flow Cyt
Use at an assay dependent concentration. PubMed: 24067955 ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
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相关性
The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002). -
细胞定位
Host cell nucleus -
数据库链接
- Entrez Gene: 956531 Influenza A
- SwissProt: P03466 Influenza A
- SwissProt: P69291 Influenza A
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别名
- Common flu NP antibody
- Influenza A virus NP antibody
- NP antibody
see all
图片
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Immunofluorescence assay of MDCK cells derived from canine kidney cells, and A549 cells derived from human lung carcinoma cells, that were infected with H1N1 influenza virus (A/PuertoRico/8/34). Samples were taken at 3, 9, and 24 hours post-infection. Influenza A Virus Nucleoprotein antibody (ab128193) efficiently detected virus-infected MDCK and A549 cells as early as 3 h after infection. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an green flurescent secondary antibody.
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Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/1000 dilution + MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34
Secondary
Rabbit Anti-Mouse IgG H&L (HRP) (ab97046) at 1/10000 dilution -
Immunofluorescence assay of 293T cells expressing HA or NP of pandemic (H1N1) 2009 influenza A virus (A/Suita/1/2009). Influenza A Virus Nucleoprotein antibody (ab128193) specifically recognized NP-expressing cells while a proprietary mouse anti-HA monoclonal antibody specifically recognized HA.
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All lanes : Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/300 dilution
Lanes 1 & 6 & 11 : MDCK cells mock infected
Lane 2 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 3 hrs post-infection
Lane 3 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 9 hrs post-infection
Lane 4 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 24 hrs post-infection
Lane 5 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 48 hrs post-infection
Lane 7 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 3 hrs post-infection
Lane 8 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 9 hrs post-infection
Lane 9 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 24 hrs post-infection
Lane 10 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 48 hrs post-infection
Lane 12 : MDCK cells infected H5N1
(A/duck/HK/342/78)collected 3 hrs post-infection
Lane 13 : MDCK cells infected with H5N1
(A/duck/HK/342/78) collected 9 hrs post-infection
Lane 14 : MDCK cells infected with H5N1
(A/duck/HK/342/78) collected 24 hrs post-infection
Lane 15 : MDCK cells infected with H5N1
(A/duck/HK/342/78) collected 48 hrs post-infection
Tubulin was used as loading control.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (33)
ab128193 被引用在 33 文献中.
- Liu HY et al. Single-virus tracking with quantum dots in live cells. Nat Protoc 18:458-489 (2023). PubMed: 36451053
- Wu W et al. MicroRNA let-7 Suppresses Influenza A Virus Infection by Targeting RPS16 and Enhancing Type I Interferon Response. Front Cell Infect Microbiol 12:904775 (2022). PubMed: 35873150
- John SP et al. Small-molecule screening identifies Syk kinase inhibition and rutaecarpine as modulators of macrophage training and SARS-CoV-2 infection. Cell Rep 41:111441 (2022). PubMed: 36179680
- Kelly JN et al. Comprehensive single cell analysis of pandemic influenza A virus infection in the human airways uncovers cell-type specific host transcriptional signatures relevant for disease progression and pathogenesis. Front Immunol 13:978824 (2022). PubMed: 36268025
- Pila-Castellanos I et al. Mitochondrial morphodynamics alteration induced by influenza virus infection as a new antiviral strategy. PLoS Pathog 17:e1009340 (2021). PubMed: 33596274