Anti-Influenza A Virus M2 Protein 抗体 [EPR28252-11] - BSA and Azide free

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] - BSA and Azide free (AB313890)

This data was developed using ab313889, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human tissue labeling Influenza A Virus M2 Protein with ab313889 at 1/4000 (0.132 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive on (A) HEK-293T transfected with an Influenza A virus M2 expression vector containing a myc tag; no staining on (B) HEK-293T transfected with an empty vector. The section was incubated with ab313889 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] - BSA and Azide free (AB313890)

This data was developed using ab313889, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Influenza A Virus M2 Protein with ab313889 at 1/100 (5.26 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing membranous and cytoplasmic staining in 293T cells transfected with an Influenza A virus M2 expression vector containing a myc tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain at 1/100 (5 µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] - BSA and Azide free (AB313890)

This data was developed using ab313889, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Influenza A Virus M2 Protein with ab313889 at 1/4000 (0.132 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on human cerebrum.The section was incubated with ab313889 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] - BSA and Azide free (AB313890)

This data was developed using ab313889, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (human embryonic kidney epithelial cell) transfected with an Influenza A virus M2 expression vector containing a myc tag cells labelling Influenza A Virus M2 Protein with ab313889 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) . A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells are co-stained with Myc tag conjugated to Alexa Fluor®647.

• I-ELISA

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Indirect ELISA - Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] - BSA and Azide free (AB313890)

This data was developed using ab313889, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab313889 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution. Antigen : Influenza A Virus M2 Protein peptide1, Influenza A Virus M2 Protein peptide2. Antigen concentration : 1000 ng/ml

• WB

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Western blot - Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] - BSA and Azide free (AB313890)

This data was developed using ab313889, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution Exposure time : 59 seconds

All lanes:

Western blot - Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] (<a href='/products/primary-antibodies/influenza-a-virus-m2-protein-antibody-epr28252-11-ab313889'>ab313889</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with an Influenza A virus M2 expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 20 kDa

false

Exposure time: 59s