抗甲型流感Virus M2蛋白抗体[14C2] (ab5416)
Key features and details
- Mouse monoclonal [14C2] to Influenza A Virus M2 Protein
- Suitable for: ICC
- Reacts with: Influenza A
- Isotype: IgG1
参阅全部 Influenza A Virus M2 Protein 一抗
描述小鼠单克隆抗体[14C2] to甲型流感Virus M2蛋白
经测试应用适用于: ICCmore details
种属反应性与反应: Influenza A
Full length protein corresponding to Influenza A Virus M2 Protein. Full length M2 protein from A/WSN/33-infected CV1 cell lysate.
表位Detects the N-terminal of the Influenza A Virus M2 Protein.
- ICC/IF: Infected MDCK cells with Influenza A.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Concentration information loading...
纯度Protein G purified
Primary antibody说明Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.
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Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml.
相关性The Matrix protein M2 forms a protons channel. When the environmental pH is lower than a threshold, the M2 channel is activated and selectively transports protons accross the membrane from the extracellular side to the cytoplasmic side. It is crucial for the uncoating process. When the virion is internalized into the endosome the channel can acidify the virion interior, promoting the dissociation of the viral matrix protein (M1) from the ribonucleoprotein (RNP) thus allowing the transport of the RNP from the virion into the cell’s nucleus. For some influenza virus subtypes, the M2 channel can elevate the intravesicular pH of the trans Golgi network, preventing the viral protein haemagglutinin, which is transported to the cell surface through the trans Golgi network, from incorrect maturation in an otherwise low pH environment.
细胞定位Virion membrane. Apical cell membrane; Single-pass type III membrane protein.
- Entrez Gene: 1461002 Influenza A
- Entrez Gene: 956528 Influenza A
- SwissProt: A0A2R3YRM7 Influenza A
- SwissProt: P06821 Influenza A
- SwissProt: Q67179 Influenza A
- Influenza A virus matrix protein M2 antibody
- M antibody
- M2 antibody
ab5416 被引用在 68 文献中.
- Pila-Castellanos I et al. Mitochondrial morphodynamics alteration induced by influenza virus infection as a new antiviral strategy. PLoS Pathog 17:e1009340 (2021). PubMed: 33596274
- Colant N et al. Escherichia Coli-Based Cell-Free Protein Synthesis for Iterative Design of Tandem-Core Virus-Like Particles. Vaccines (Basel) 9:N/A (2021). PubMed: 33669126
- Zhang W et al. Trojan Horse Delivery of 4,4'-Dimethoxychalcone for Parkinsonian Neuroprotection. Adv Sci (Weinh) 8:2004555 (2021). PubMed: 33977069
- Durgan J et al. Non-canonical autophagy drives alternative ATG8 conjugation to phosphatidylserine. Mol Cell 81:2031-2040.e8 (2021). PubMed: 33909989
- Villalón-Letelier F et al. MARCH8 Restricts Influenza A Virus Infectivity but Does Not Downregulate Viral Glycoprotein Expression at the Surface of Infected Cells. mBio 12:e0148421 (2021). PubMed: 34517760