AB302939

重组抗甲型流感H7N9 Neuraminidase抗体[10F4] - BSA and Azide Free

Anti-Influenza A H7N9 Neuraminidase antibody [10F4] - BSA and Azide Free

Recombinant
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Mouse Recombinant Monoclonal Influenza A H7N9 Neuraminidase antibody. Carrier free. Suitable for ICC/IF, I-ELISA and reacts with Transfected cell line - Influenza A, Influenza A samples.

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Influenza A H7N9 Neuraminidase antibody [10F4] - BSA and Azide Free (AB302939)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Influenza A H7N9 Neuraminidase antibody [10F4] - BSA and Azide Free (AB302939)

This data was developed using ab302938, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293T (human embryonic kidney epithelial cell) cells labeling Influenza A H7N9 Neuraminidase with ab302938 at 1/50 dilution (21.86 µg/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2µg/mL) (Green). Confocal image showing membrane staining in HEK-293T cells transfected with a mouse N9, H7N9, A/Anhui/1/2013 expression vector containing a myc tag. Anti-HA.11 Epitope Tag mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (5µg/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2µg/mL).

Indirect ELISA - Anti-Influenza A H7N9 Neuraminidase antibody [10F4] - BSA and Azide Free (AB302939)

I-ELISA

Supplier Data

Indirect ELISA - Anti-Influenza A H7N9 Neuraminidase antibody [10F4] - BSA and Azide Free (AB302939)

This data was developed using ab302938, the same antibody clone in a different buffer formulation. Primary antibody ab302938 used at 0-2000 ng/ml. Antigen concentration 5000 ng/ml. Secondary antibody is Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at a 1/1000 dilution.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

10F4

亚型

IgG1

不含载体蛋白

Yes

反应种属

Influenza A

应用

ICC/IF, I-ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Transfected cell line - Influenza A": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }

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产品详情

ab302939 is a carrier free version of ab302938.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Influenza A H7N9 Neuraminidase also known as NA or sialidase is an enzyme important in the viral replication process of the influenza virus. This protein with a molecular mass of approximately 47 kDa is expressed on the surface of the influenza A virus particles. Neuraminidase facilitates the removal of sialic acid residues from glycoproteins and glycolipids which is essential for the release of new viral particles from infected host cells. The enzymatic action is an important factor in the virus's spread within the respiratory tract of the host organism.

Biological function summary

The action of Influenza A H7N9 Neuraminidase assists in the completion of the viral life cycle. Neuraminidase in conjunction with hemagglutinin is part of a complex that allows the virus to attach and penetrate host cells efficiently. By cleaving sialic acid residues neuraminidase enables the newly formed viral particles to detach and spread facilitating infection progression. This protein's ability to disrupt cellular interactions highlights its role in viral pathogenicity an essential feature for the influenza virus's survival in host organisms.

Pathways

Neuraminidase plays a significant part in the influenza viral entry and propagation pathways. Its interaction with hemagglutinin is important for the entry of the virus into host cells. Neuraminidase's activity supports the recycling of viral particles therefore contributing to the greater viral lifecycle network. Additionally its function links with pathways of immune evasion where it helps in avoiding the host's immune response primarily through altering host cell surface molecules and aiding in the spread of infection.

Neuraminidase is closely associated with the pathogenesis of influenza particularly with influenza A and its subtypes such as H7N9. Neuraminidase inhibitors like oseltamivir target this protein to prevent the spread of influenza virus and mitigate symptoms. Resistance to such inhibitors can arise making the study of neuraminidase important for developing effective treatments. Neuraminidase's activity affects both the viral lifecycle and the host's immune response underlining its role in the interactions between influenza virus proteins and host immune components.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information Influenza A H7N9 Neuraminidase

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组抗甲型流感H7N9 Neuraminidase抗体[10F4] - BSA and Azide Free

Anti-Influenza A H7N9 Neuraminidase antibody [10F4] - BSA and Azide Free

Immunocytochemistry/ Immunofluorescence - Anti-Influenza A H7N9 Neuraminidase antibody [10F4] - BSA and Azide Free (AB302939)

Indirect ELISA - Anti-Influenza A H7N9 Neuraminidase antibody [10F4] - BSA and Azide Free (AB302939)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原