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AB318988

重组抗甲型流感H1N1 (A/California/07/2009) neuraminidase抗体[CD6]

Anti-influenza A H1N1 (A/California/07/2009) neuraminidase antibody [CD6]

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Mouse Recombinant Monoclonal N1, influenza H1N1, A/California/07/2009 antibody. Suitable for ICC/IF, I-ELISA and reacts with Transfected cell line - Influenza A virus (A/California/07/2009(H1N1)), Recombinant full length protein - Influenza A virus (A/California/07/2009(H1N1)) samples.

查看别名

C3W6G3, C3W6G3_9INFA, Neuraminidase, N1, influenza H1N1, A/California/07/2009

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-influenza A H1N1 (A/California/07/2009) neuraminidase antibody [CD6] (AB318988)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-influenza A H1N1 (A/California/07/2009) neuraminidase antibody [CD6] (AB318988)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling influenza A H1N1 (A/California/07/2009) neuraminidase with ab318988 at 1/2000 (0.465 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing cytoplasmic and membranous staining in 293T cells transfected with a N1, H1N1, A/California/07/2009 expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a N9, H7N9, A/Anhui/1/2013 or N1, H1N1, A/Brisbane/59/2007 vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
A : Myc transfected cells
B : N1, H1N1, A/Brisbane/59/2007-Myc transfected cells
C : N1, H1N1, A/California/07/2009-Myc transfected cells
D : N9, H7N9, A/Anhui/1/2013-Myc transfected cells
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Magenta). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2ug/ml) dilution.

Indirect ELISA - Anti-influenza A H1N1 (A/California/07/2009) neuraminidase antibody [CD6] (AB318988)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-influenza A H1N1 (A/California/07/2009) neuraminidase antibody [CD6] (AB318988)

Indirect ELISA analysis of ab318988 at 2000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution. Antigen : N1, influenza H1N1, A/California/07/2009. Antigen concentration : 0-1000 ug/ml.

不同偶联物与剂型 (1)

  • Carrier free

    Anti-influenza A H1N1 (A/California/07/2009) neuraminidase antibody [CD6] - BSA and Azide free

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

CD6

亚型

IgG2a

不含载体蛋白

No

反应种属

Influenza A virus (A/California/07/2009(H1N1))

应用

ICC/IF, I-ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody reacts with Influenza A virus H1N1, A/California/07/2009 neuraminidase and does not react with Influenza A virus H1N1, A/Brisbane/59/2007 neuraminidase or Influenza A virus H7N9, A/Anhui/1/2013 neuraminidase.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A virus (A/California/07/2009(H1N1))": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Influenza A virus (A/California/07/2009(H1N1))": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "2000 ng/mL", "IELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Influenza A virus (A/California/07/2009(H1N1))": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Influenza A H1N1 (A/California/07/2009) neuraminidase also known as NA is an enzyme important for the viral life cycle. It cleaves sialic acid groups from glycoproteins facilitating the release of new virus particles from host cells. This process aids the spread of infection within the host. Neuraminidase of H1N1 has a molecular mass of around 60 kDa. It is expressed on the surface of the influenza virus where it functions to support the viral budding process.
Biological function summary

Neuraminidase enables the influenza virus to exit infected cells and invade new ones ensuring efficient viral dissemination. It functions as part of a complex with the viral hemagglutinin protein which helps the virus attach to the host cell surface before infection. Neuraminidase's enzymatic action counteracts hemagglutinin's binding by cutting sialic acid residues from host receptors an essential function for viral replication.

Pathways

Neuraminidase plays a role in the influenza viral budding and release pathway coordinating with hemagglutinin to manage the balance between virus attachment and release. This action relates to various antiviral research efforts aimed at blocking this process focusing on the hemagglutinin-neuraminidase balance. Interventions at this level may inhibit the virus' ability to proliferate highlighting the importance of neuraminidase in therapeutic pathways targeting influenza.

Neuraminidase is directly linked to influenza infection a respiratory illness with pandemic potential. Inhibitors like oseltamivir and zanamivir are designed to block neuraminidase activity preventing the virus' spread in the body. These inhibitors target the interaction between neuraminidase and sialic acid residues aiming to disrupt the pathogenesis of the influenza virus. This interaction offers a promising target for therapeutic strategies against influenza virus strains including potential drug-resistant variants.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information N1, influenza H1N1, A/California/07/2009
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Abcam Product Promise

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