Anti-INF2抗体(ab121114)
Key features and details
- Rabbit polyclonal to INF2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-INF2抗体
参阅全部 INF2 一抗 -
描述
兔多克隆抗体to INF2 -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human INF2 aa 50-250.
Database link: Q27J81 -
阳性对照
- IHC-P: INF2 transfected HEK293T cells, Human stomach tissue, U-251MG cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121114于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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| 说明 |
|---|
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
靶标
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功能
Severs actin filaments and accelerates their polymerization and depolymerization. -
组织特异性
Widely expressed. In the kidney, expression is apparent in podocytes and some tubule cells. -
疾病相关
Defects in INF2 are the cause of focal segmental glomerulosclerosis type 5 (FSGS5) [MIM:613237]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. -
序列相似性
Belongs to the formin homology family.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
Contains 1 WH2 domain. -
结构域
The WH2 domain acts as the DAD (diaphanous autoregulatory) domain and binds to actin monomers.
Regulated by autoinhibition due to intramolecular GBD-DAD binding.
The severing activity is dependent on covalent attachment of the FH2 domain to the C-terminus. -
细胞定位
Cytoplasm > perinuclear region. - Information by UniProt
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数据库链接
- Entrez Gene: 64423 Human
- Omim: 610982 Human
- SwissProt: Q27J81 Human
- Unigene: 24956 Human
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别名
- C14orf151 antibody
- C14orf173 antibody
- CMTDIE antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INF2 antibody (ab121114)
Immunohistochemical analaysis of human stomach tissue labelling INF2 with ab121114 at 1/50 dilution. Moderate to strong cytoplasmic positivity in glandular cells is shown.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INF2 antibody (ab121114)Immunohistochemical analaysis of human kidneytissue labelling INF2 with ab121114 at 1/50 dilution. Moderate cytoplasmic positivity in podocytes and cells in tubules is shown.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INF2 antibody (ab121114)Immunohistochemical analaysis of human skeletal muscle labelling INF2 with ab121114 at 1/50 dilution. Moderate cytoplasmic positivity in neurons is shown.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INF2 antibody (ab121114)Immunohistochemical analaysis of human skeletal muscle labelling INF2 with ab121114 at 1/50 dilution. No cytoplasmic postitivity is shown in striated muscle fibres.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab121114 尚未被引用在任何文献中。