重组Anti-Indoleamine 2，3-dioxygenase抗体[EPR1230Y] - BSA and Azide free (ab271859)
![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] - BSA and Azide free (ab271859)](https://www.abcam.cn/ps/products/271/ab271859/Images/ab271859-548210-anti-indoleamine-2-3-dioxygenase-antibody-epr1230y-western-blot-wildtype-a549-treated-ifn-gamma-25-ngml-48-h-ab281500-wildtype-a549-v.jpg "Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] - BSA and Azide free (ab271859)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1230Y] to Indoleamine 2, 3-dioxygenase - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Indoleamine 2，3-dioxygenase抗体[EPR1230Y] - BSA and Azide free
参阅全部 Indoleamine 2, 3-dioxygenase 一抗 -
描述
兔单克隆抗体[EPR1230Y] to Indoleamine 2，3-dioxygenase - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Wild-type A549 Treated IFN gamma, SK-OV-3, and HeLa treated with 10ng/ml IFN-gamma for 24 hours whole cell lysate.
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常规说明
ab271859 is the carrier-free version of ab76157.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1230Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab271859于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa. |
靶标
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功能
Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. -
通路
Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2. -
序列相似性
Belongs to the indoleamine 2,3-dioxygenase family. - Information by UniProt
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数据库链接
- Entrez Gene: 3620 Human
- Omim: 147435 Human
- SwissProt: P14902 Human
- Unigene: 840 Human
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别名
- 3-dioxygenase antibody
- I23O1_HUMAN antibody
- IDO 1 antibody
see all
图片
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Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] - BSA and Azide free (ab271859)All lanes : Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/2500 dilution
Lane 1 : Wild-type A549 Treated IFN gamma (25 ng/mL, 48 h) ab281500 cell lysate
Lane 2 : Wild-type A549 Vehicle Control IFN gamma (0 ng/mL, 48 h) ab255554 cell lysate
Lane 3 : IDO1 knockout A549 Treated IFN gamma (25 ng/mL, 48 h) ab281489 cell lysate
Lane 4 : IDO1 knockout A549 Vehicle Control IFN gamma (0 ng/mL, 48 h) ab263515 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 42-56 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab76157).
Western blot: Anti-IDO1 antibody [EPR1230Y] (ab76157) staining at 1/2500 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab76157 was shown to bind specifically to IDO1. A band was observed at 42/56 kDa in treated wild-type A549 cell lysates with no signal observed at this size in IDO1 knockout cell line. To generate this image, wild-type and IDO1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] - BSA and Azide free (ab271859)](https://www.abcam.cn/ps/products/271/ab271859/Images/ab271859-469686-anti-indoleamine-2-3-dioxygenase-antibody-epr1230y-bsa-and-azide-free-western-blot-wildtype-a549-vehicle-control-ifn-gamma-0-ngml-48-h-wildtype-a549-trea.jpg)
Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] - BSA and Azide free (ab271859)All lanes : Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/2500 dilution
Lane 1 : Wild-type A549 Vehicle Control IFN gamma (0 ng/ml, 48 h) cell lysate
Lane 2 : Wild-type A549 Treated IFN gamma (25 ng/ml, 48 h) cell lysate
Lane 3 : IDO1 knockout A549 Vehicle Control IFN gamma (0 ng/ml, 48 h) cell lysate
Lane 4 : IDO1 knockout A549 Treated IFN gamma (25 ng/ml, 48 h) cell lysate
Lane 5 : SK-OV-3 cell lysate
Lane 6 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab76157).
Lanes 1 - 6: Merged signal (red and green). Green - ab76157 observed at 40 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab76157 was shown to react with Indoleamine 2, 3-dioxygenase in treated wild-type A549 cells in Western blot with loss of signal observed in treated IDO1 knockout cell line ab266949 (IDO1 knockout cell lysate ab256948). Wild-type A549 and IDO1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab76157 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 2500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] - BSA and Azide free (ab271859)](https://www.abcam.cn/ps/products/271/ab271859/Images/ab271859-449624-anti-indoleamine-2-3-dioxygenase-antibody-epr1230y-westernblot-hela-human.jpg)
Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] - BSA and Azide free (ab271859)Lane 1 : Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/1000 dilution
Lane 2 : Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10ng/ml IFN-gamma for 24 hours whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45 kDaThe expression of Indoleamine 2, 3-dioxygenase can be upregulated by IFN-gamma (PMID: 29685162). We are unsure how to define the extra bands.
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![Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] - BSA and Azide free (ab271859)](https://www.abcam.cn/ps/products/271/ab271859/Images/ab271859-2-benefits-of-recombinant-antibodies.png)
Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] - BSA and Azide free (ab271859)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab271859 尚未被引用在任何文献中。
